Hofmeister　effect　has　been　used　to　prepare　highly　tough　and　conductive　hydrogel.　However,　it　usually　needs　the　complex　steps　of　post-treatment　of　a　pre-formed　weak　hydrogel　by　soaking　in　salt　solution.　Herein,　strong　and　tough　silk　fibroin/poly(vinyl　alcohol)　(SF/PVA)　ionic　conductive　hydrogel　was　prepared　by　a　simple　one-pot　method　by　direct　introduction　of　sodium　citrate　(Na3Cit)　into　SF/PVA　solution.　It　was　found　that　Na3Cit　could　not　be　directly　introduced　into　SF/PVA　solution　due　to　its　salting-out　effect　to　prepare　tough　hydrogel.　Fortunately,　the　salting-out　effect　of　Na3Cit　could　be　weakened　with　the　presence　of　calcium　chloride　(CaCl2)　and　glycerol　in　SF/PVA　solution.　Finally,　the　strong　and　tough　SF/PVA　hydrogel　was　obtained　by　the　one-time　freezing/thawing　process　of　SF/PVA/CaCl2/Na3Cit/glycerol　solution.　The　tough　SF/PVA/CaCl2/Na3Cit/glycerol　organohydrogel　with　the　tensile　strength　of　0.33 MPa　and　elongation-at-break　of　540%　were　obtained.　The　SF/PVA/CaCl2/Na3Cit/glycerol　organohydrogel　with　the　excellent　mechanical　properties　and　ionic　conductivity　was　used　as　the　strain　sensor.　The　SF/PVA/CaCl2/Na3Cit/glycerol　organohydrogel　sensor　could　detect　various　human　motions　with　high　sensitivity　(GF　=　6.87).　The　SF/PVA/CaCl2/Na3Cit/glycerol　organo　hydrogel　strain　sensor　could　generate　reliable　and　reproducible　electrical　signals　in　response　to　various　mechanical　stimuli.　We　intend　to　present　a　simple　and　effective　method　to　prepare　high　performance　ionic　hydrogel　by　Hofmeister　effect.　Graphical　Abstract:　(Figure　presented.)　©　Iran　Polymer　and　Petrochemical　Institute　2024.