Combining　molecular　imprinting　technology　with　electrostatic　spinning　technology,　a　molecularly　imprinted　nanofiber　membrane　material　with　memory　and　recognition　of　specific　imprinted　molecules　is　thus　produced.　It　has　the　advantages　of　high　efficiency,　reusability,　easy　amplification,　and　simple　operation.　In　this　study,　alkane　molecularly　imprinted　nanofibrous　membranes　are　prepared　as　a　carrier　for　immobilize　Bacillus　cereus　LY-1　for　the　degradation　of　diesel　in　oil–water　system.　The　adsorption　capacity　of　the　molecularly　imprinted　material　for　diesel　oil　with　n-decyl　alcohol　as　the　template　molecule　reach　120.25　mg　g−1,　the　adsorption　capacity　of　alkane　molecularly　imprint　nanofiber　membrane　prepared　using　polyvinyl　alcohol　(PVA)　as　the　membrane　substrate　material　is　41.37　mg　g−1.　After　4　days,　the　immobilized　LY-1　cells　eliminate　roughly　80.7%　of　the　diesel　at　a　starting　concentration　of　3　g　L−1.　The　results　show　that　imprinted　structures　in　MINM　enhance　degradation　by　loaded　microorganisms.　©　2024　Wiley　Periodicals　LLC.