The　extraction　of　collagen　for　packaging　films　typically　requires　a　time-consuming　process　and　the　use　of　substantial　chemicals.　Herein,　we　present　a　full　life　cycle　green　preparation　method　for　rapidly　producing　collagen-based　food　packaging　films　using　Halocynthia　roretzi　(HR),　a　collagen-rich　marine　organism,　as　raw　material.　We　first　prepared　the　micro/nano-sized　collagen　fibers　from　HR　tissue　by　utilizing　urea　and　sonication　as　effective　hydrogen-bond　breakers.　Subsequently,　the　collagen　fiber　was　rapidly　fabricated　into　a　film　through　vacuum　filtration.　The　resulting　collagen　fiber　film　(CFF)　exhibited　a　uniform　and　dense　surface,　along　with　good　tensile　properties,　water　resistance,　and　biodegradability.　In　addition,　the　deposition　of　chitosan　(CS)　on　the　surface　of　CFF　resulted　in　a　remarkable　preservation　effect　for　both　strawberries　and　pork.　This　full　life　cycle　preparation　method　for　collagen-based　films　provides　a　promising　and　innovative　approach　to　the　sustainable　preparation　of　food　packaging　films.　©　2024　Elsevier　Ltd