Tebuconazole　is　one　of　the　most　commonly　used　fungicides　in　agricultural　production,　that　has　the　merits　of　highly　effectiveness,　broad　spectrum　and　systemic　function.　Excessive　tebuconazole　may　pose　a　great　threat　to　human　and　animal　health.　Traditional　detection　techniques　for　tebuconazole　usually　have　limitations　such　as　expensive　equipment,　poor　antibody　stability,　and　time-consuming　procedures.　Herein,　a　sensitive　sensor　is　developed　for　the　rapid　detection　of　tebuconazole　based　on　hydrogel　surface-enhanced　Raman　scattering　(SERS)　chips.　Aggregated　Ag　nanoparticles　(a-AgNPs)　with　tunable　localized　surface　plasmon　resonance　(LSPR)　wavelength　are　in-situ　synthesized　in　polyvinyl　alcohol　(PVA)　solution　for　preparing　hydrogel　SERS　chips.　Three　hydrogel　SERS　chips　are　obtained　to　match　the　three　commonly　used　laser　wavelengths.　On　the　basis,　a　match　laser　wavelength　is　selected　according　to　the　energy　levels　of　tebuconazole　and　the　Fermi　level　of　a-AgNPs　to　gain　a　strong　chemical　enhancement.　At　the　same　time,　the　chip　with　a　corresponding　LSPR　wavelength　to　the　laser　is　applied　to　obtain　a　strong　electromagnetic　enhancement.　Thus,　highly　sensitive　SERS　signal　of　tebuconazole　is　obtained.　Furthermore,　the　obtained　hydrogel　SERS　chips　have　good　repeatability,　outstanding　reproducibility　and　strong　anti-interference　ability,　and　show　outstanding　reliability　in　practical　applications.　As　a　result,　the　SERS　chips　offer　a　reliable　and　convenient　platform　for　the　quick　detection　of　tebuconazole　in　foods.　The　detection　limit　is　as　low　as　1　ppb,　and　the　recoveries　is　distributed　in　the　range　of　94.66–106.70　%.　This　work　would　promote　greatly　the　application　of　SERS　in　small　molecule　detection.　©　2024