The　rapid　development　of　modern　agriculture　generally　relies　on　the　massive　use　of　pesticides，which　also　become　the　main　organic　pollutants　in　the　environment　due　to　the　structural　stability　and　high　toxicity　effects　of　pesticide　molecules.　In　this　study，IRMOF-3　with　high　fluorescence　efficiency　was　prepared　by　a　simple　and　rapid　room-temperature　synthesis　method，and　then　applied　to　the　fluorescence　detection　and　analysis　of　the　toxic　neonicotinoid　pesticide，nitenpyram（NIT）.　The　results　show　that　the　prepared　IRMOF-3　is　a　pure-phase　crystalline　material　with　stable　photoluminescence　at　427　nm；The　blue　fluorescence　of　IRMOF-3　can　be　quenched　quickly　by　NIT　in　the　aqueous　environment，which　shows　a　good　selectivity　and　a　strong　anti-interference　ability　among　many　interferences.　The　fluorescence　analytical　method　developed　by　this　method　showed　a　significant　correlation　in　the　concentration　range　of　0~38　μmol/L　of　NIT，and　the　limit　of　detection（LOD）was　as　low　as　0.35　μmol/L.　The　spiked　recoveries　demonstrated　that　the　method　had　good　recoveries（95.16%~106.51%）with　the　relative　standard　deviations（RSDs）≤　6.68%.　The　analysis　of　the　multiple　characterization　results　indicated　that　the　fluorescence　detection　mechanism　mainly　refers　to　the　energy　competition　absorption　of　NIT　for　the　excitation　light.　In　addition，the　fluorescence　quenching　due　to　electron　transfer　is　also　one　of　the　fluorescence　detection　mechanisms　of　IRMOF-3　for　NIT.　In　conclusion，IRMOF-3　based　on　fluorescence　analysis　has　realized　the　detection　of　NIT　with　high　sensitivity，which　provides　a　simple　and　reliable　analytical　method　for　the　future　detection　of　NIT-type　pollution　in　water　environment.　©　2024　Journal　of　Zhongshan　University.　All　rights　reserved.