Establishment of an A/T-Rich Specifically MGB Probe digital droplet PCR Assays Based on SNP for Brucella wild strains and vaccine strains - Details

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Abstract：

In　recent　years,　immunization　with　the　S2　live-attenuated　vaccine　has　been　recognized　as　the　most　economical　and　effective　strategy　for　preventing　brucellosis　in　Inner　Mongolia,　China.　However,　there　are　still　challenges　related　to　vaccine　toxicity　and　the　inability　to　distinguish　between　vaccine　immunization　and　natural　infection.　Therefore,　in　this　study,　we　developed　a　digital　droplet　polymerase　chain　reaction　(ddPCR)　assay　based　on　single-nucleotide　polymorphism　(SNP)　loci　to　identify　wild　Brucella　strains　and　S2　vaccine　strains.　The　assay　demonstrated　excellent　linearity　(R2＞　0.99)　with　a　lower　detection　limit　of　10　copies/µL　for　both　wild　and　vaccine　strains.　Additionally,　the　ddPCR　assay　outperformed　the　real-time　fluorescent　quantitative　PCR　(qPCR)　assay　in　screening　50　clinical　samples.　We　have　established　an　effective　and　highly　sensitive　ddPCR　assay　for　Brucella,　providing　an　efficient　method　for　detecting　and　differentiating　wild　strains　of　Brucella　from　the　S2　vaccine　strain.　©　2024　The　Author(s)

Keyword：

Brucella Digital droplet polymerase chain reaction S2 Vaccine strain Single-nucleotide polymorphism Wild strains

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[ 1 ] [Li W.]Medical College, Inner Mongolia Minzu University, Tongliao, 028000, China
[ 2 ] [Zhang S.]Medical College, Inner Mongolia Minzu University, Tongliao, 028000, China
[ 3 ] [Dang S.]Keerqin District First People's Hospital, Tongliao, 028000, China
[ 4 ] [Gao L.]Tongliao Infectious Disease Hospital, Tongliao, 028000, China
[ 5 ] [Li G.]Tongliao Infectious Disease Hospital, Tongliao, 028000, China
[ 6 ] [Cheng D.]Beidahuang Industry Group General Hospital, Harbin, 150000, China
[ 7 ] [Jiang L.]College of Chemistry, Fuzhou University, Fuzhou, 350000, China
[ 8 ] [Huang T.]College of Public Health, Inner Mongolia Minzu University, Tongliao, 028000, China
[ 9 ] [Huang T.]Brucellosis Prevention and Treatment Engineering Research Center of Inner Mongolia Autonomous Region, Tongliao, 028000, China
[ 10 ] [Huang T.]Key Laboratory of Zoonose Prevention and Control at Universities of Inner Mongolia Autonomous Region, Tongliao, 028000, China
[ 11 ] [Zhai J.]Medical College, Inner Mongolia Minzu University, Tongliao, 028000, China
[ 12 ] [Zhai J.]Brucellosis Prevention and Treatment Engineering Research Center of Inner Mongolia Autonomous Region, Tongliao, 028000, China
[ 13 ] [Zhai J.]Key Laboratory of Zoonose Prevention and Control at Universities of Inner Mongolia Autonomous Region, Tongliao, 028000, China

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Diagnostic Microbiology and Infectious Disease

ISSN： 0732-8893

Year： 2024

Issue： 1

Volume： 110

2 . 1 0 0

JCR@2023

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SCOPUS Cited Count： 1

ESI Highly Cited Papers on the List： 0 Unfold All

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