Gelatin　and　sodium　alginate　(SA)　are　two　important　biological　macromolecules,　exhibiting　excellent　biocompatibility　and　gel-forming　ability.　However,　traditional　SA　and　gelatin　hydrogel　displays　limited　mass　transport,　low　porosity,　instability,　and　poor　mechanical　properties　extremely　restricted　their　therapeutic　effect　and　application　scenarios.　Herein,　microbial　fermentation　and　synergistic　toughening　strategies　were　used　for　preparing　macroporous　and　tough　hydrogel.　The　study　investigated　the　fermentation　and　toughening　conditions　of　hydrogel.　The　hydrogel　composed　of　CaCl2　cross-linked　physically　network　and　EDC/NHS　cross-linked　covalently　network,　exhibiting　significantly　improved　mechanical　properties,　and　excellent　recovery　efficiency.　In　addition,　the　hydrogel　has　a　hierarchical　macroporous　structure　of　100-500　mu　m,　demonstrating　high　porosity　of　10　times,　swelling　rate　of　1541.0　%,　and　high　mass　infiltration　capability.　Further,　after　Ag+　treatment,　the　macroporous　hydrogel　dressing　showed　outstanding　biocompatibility.　Compared　with　non-porous　hydrogel,　the　resulting　macroporous　hydrogel　dressing　displayed　high　antibacterial　and　antioxidant　properties.　It　could　effectively　alleviate　intracellular　ROS　formation　induced　by　H2O2.　In　vivo　experiments　indicated　that　it　has　significantly　better　effect　than　non-porous　hydrogel　in　accelerating　wound　healing.　The　overall　results　suggest　that　the　gelatin/SA-based　macroporous　and　tough　hydrogel　proposed　in　this　study　holds　excellent　prospects　for　application　in　wound　dressings.