• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Cheng, Lingjun (Cheng, Lingjun.) [1] | Yang, Yuanyuan (Yang, Yuanyuan.) [2] | Lin, Shaobin (Lin, Shaobin.) [3] | Su, Canping (Su, Canping.) [4] | You, Mingming (You, Mingming.) [5] | Liu, Yating (Liu, Yating.) [6] | He, Qingfei (He, Qingfei.) [7] | Chen, Jiaming (Chen, Jiaming.) [8] | Lin, Zhenyu (Lin, Zhenyu.) [9] (Scholars:林振宇) | Hong, Guolin (Hong, Guolin.) [10]

Indexed by:

EI

Abstract:

Background: Gold nanoclusters (AuNCs) obtained by electroreduction have excellent electrochemiluminescence (ECL) properties, and its ECL intensity is regulated by the valence state. In addition, their ECL signals can be rapidly quenched by reactive oxygen species (ROS). Based on this observation, a sensitive ROS biosensor was designed based on valence regulation of AuNCs. Excessive ROS in seminal plasma can lead to male infertility, and the short half-life and instability of ROS pose a challenge for their detection. Since valence regulation can be done quickly and is very sensitive, this ECL biosensor holds promise to address this issue. Results: The ECL mechanism of AuNCs and the quenching mechanism of AuNCs by ROS were explored, mainly because ROS change the valence state of AuNCs. The ECL signals of the biosensor have a linear relationship with logarithm of the target concentration in the range of 1.0 × 10−8 to 1.0 × 10−3 M and 1.0 × 10−3 to 1.0 × 10−1 M, with a detection limit of 0.75 × 10−10 M (S/N = 3). The biosensor enables rapid one-step detection of ROS and has the advantage of being stable and reusable. More notably, the results of 57 real samples showed that the biosensor can be used to accurately assess the concentration of seminal plasma ROS, guiding the monitoring of sperm quality and the diagnosis of male infertility. Significance: Compared with the traditional strategy of applying AuNCs only as a luminescent body, this strategy of regulating the valence state of AuNCs to achieve sensitive and rapid detection broadens the application of AuNCs in the field of analysis. Compared with other ROS detection strategies, the one-step immediate detection method effectively avoids the inaccuracy caused by the short half-life and natural dissipation of ROS, and is expected to improve the accuracy and efficiency of clinical diagnosis. © 2024 Elsevier B.V.

Keyword:

Nanoclay Nanoclusters Reactive oxygen species

Community:

  • [ 1 ] [Cheng, Lingjun]Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Fujian, Xiamen; 361005, China
  • [ 2 ] [Yang, Yuanyuan]Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Fujian, Xiamen; 361005, China
  • [ 3 ] [Lin, Shaobin]Xiamen Key Laboratory of Reproduction and Genetics, Department of Reproductive Medicine, Women and Children's Hospital, School of Medicine, Xiamen University, Fujian, Xiamen; 361003, China
  • [ 4 ] [Su, Canping]Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Fujian, Xiamen; 361005, China
  • [ 5 ] [You, Mingming]Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Fujian, Xiamen; 361005, China
  • [ 6 ] [Liu, Yating]Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Fujian, Xiamen; 361005, China
  • [ 7 ] [He, Qingfei]Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Fujian, Xiamen; 361005, China
  • [ 8 ] [Chen, Jiaming]Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Fujian, Xiamen; 361005, China
  • [ 9 ] [Lin, Zhenyu]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fujian, Fuzhou; 350116, China
  • [ 10 ] [Hong, Guolin]Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Fujian, Xiamen; 361005, China

Reprint 's Address:

Email:

Show more details

Related Keywords:

Source :

Analytica Chimica Acta

ISSN: 0003-2670

Year: 2024

Volume: 1330

5 . 7 0 0

JCR@2023

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

Online/Total:94/10001285
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1