We　propose　a　method　for　pixel　coordinate　correction　to　address　subtle　camera　offsets　in　an　eye-in-hand　scheme　caused　by　external　factors　in　a　machine-vision　positioning　guidance　system.　This　method　involves　establishing　a　camera-offset　pixel　coordinate　estimation　model　based　on　the　pinhole　camera　projection　model　to　eliminate　the　influence　of　camera　offset　on　the　initial　calibration　parameters.　By　dividing　the　image　region　into　subregions　and　designing　the　camera　confirmation　bit,　we　analyze　the　parameters　that　describe　the　camera-offset　model　in　each　subregion.　This　allows　for　the　determination　of　the　object　feature　points＇　pixel　coordinates　prior　to　offset　using　the　post-offset　pixel　coordinates　as　known　conditions,　thereby　ensuring　the　validity　of　the　initial　calibration　parameters.　Experimental　results　demonstrate　that　our　method　can　effectively　correct　the　pixel　coordinate　offset　errors　induced　by　changes　in　the　camera＇s　fixation　condition　with　a　resolution　of　1　pixel.