A　direct　methanol　fuel　cell　(DMFC)　is　a　promising　environmentally　friendly　energy　device,　owing　to　its　low　emission　and　high　efficiency.　Pt/C　is　the　highly　efficient　catalyst　for　DMFCs,　but　Pt　is　a　precious　metal,　and　its　weak　interaction　with　a　carbon　support　undermines　the　structure　stability　and　activity.　Herein,　two-dimensional　ultra-thin　cerium　oxide　(2D-CeO2)　was　explored　as　a　non-carbon　support　of　the　PtCu　alloy　catalyst　for　enhancing　the　electrocatalytic　performance　towards　methanol　oxidation　(MOR)　and　oxygen　reaction　(ORR)　reactions.　The　carbon-free　PtCu/2D-CeO2　catalysts　show　much　better　electro-activity　and　durability.　Pt0.25Cu/2D-CeO2　exhibits　the　highest　current　density　of　29.94　mA　cm(-2)　toward　the　MOR,　and　a　comparable　activity　to　PtCu/C　towards　the　ORR.　The　exceptional　properties,　combined　with　the　two-dimensional　ultra-thin　structure　and　high　column　of　oxygen　vacancies　of　CeO2,　make　the　PtCu　nanoparticles　more　evenly　distributed　and　more　active,　significantly　improving　the　activity　and　durability　for　the　MOR　and　ORR.