Acid　vapors　emitted　by　chemical　industries　pose　an　increasing　threat　to　public　health.　The　development　of　a　cost-effective　sensor　for　the　on-site　and　real-time　monitoring　of　environmental　acid　vapor　is　of　great　significance.　Aggregation-induced　emission　(AIE)　luminogens　overcome　the　aggregation-caused　quenching　effect　and　exhibit　intense　fluorescence　when　supported　in　the　solid　matrices.　Silica　isoporous　membrane　(SIM),　characterized　by　vertically　ordered　nanochannels,　holds　great　promise　as　a　platform　for　encapsulating　AIE　luminogens　and　enabling　gas　sensing　applications.　The　SIM　containing　surfactant　micelles　was　prepared　on　an　ITO　electrode　to　obtain　the　M-SIM/ITO,　and　Tetrakis(4-carboxyphenyl)ethylene　(TCPE)　was　employed　as　the　investigated　AIE　luminogen.　Upon　application　of　positive　potential,　the　negatively　charged　TCPE　molecules　were　driven　into　the　vertically　ordered　nanochannels,　resulting　in　observable　AIE　fluorescence.　By　investigating　the　electrophoresis　conditions　such　as　TCPE　charge,　nanochannel　microenvironment,　and　driving　electric　field,　the　AIE　mechanism　within　the　nanochannels　was　elucidated.　The　fluorescence　of　TCPE@M-SIM/ITO　exhibited　high　sensitivity　towards　acid　vapor　and　displayed　reversible　changes　during　the　absorption　and　desorption　processes.　This　behavior　can　be　attributed　to　the　SIM＇s　strong　absorption　capability　towards　acid　vapor　as　well　as　the　reversible　conversion　of　acid　vapor　on　TCPE　aggregates.　This　work　presented　an　innovative　methodology　for　studying　luminophores　within　an　orderly　nanoconfined　space,　leading　to　a　new　perspective　on　the　AIE　mechanism.　©　2024　Elsevier　B.V.