Borate　bioactive　glasses　(BGs)　have　become　indispensable　in　biomedicine　for　their　exceptional　bioactivity　and　tunable　degradation　characteristics.　This　study　presents　the　synthesis　and　comprehensive　evaluation　of　BGs　within　the　xB2O3-CaO-Na2O-K2O-MgO-P2O5　system,　featuring　varying　compositions　of　B2O3　(x　=　40,　55,　and　70　mol.%),　using　both　sol-gel　and　melting　techniques.　A　systematic　investigation　of　their　structural　evolution,　degradation　kinetics,　apatite-forming　capabilities,　and　cytotoxicity　has　been　conducted.　The　results　demonstrate　that　the　borate　BGs　with　elevated　B2O3　content　significantly　accelerates　the　degradation　rate　and　enhances　bioactivity　across　both　synthesis　methods,　as　B2O3　content　increases　from　40　to　70　mol%.　Notably,　the　sol-gel　derived　BG　samples　demonstrate　pronounced　degradation,　with　mass　loss　reaching　up　to　90　%,　and　superior　hydroxyapatite　formation　in　simulated　body　fluid,　surpassing　the　performance　of　their　melting-derived　counterparts.　Cytotoxicity　assays　with　MC3T3-E1　cells　reveal　no　significant　inhibitory　effects　from　any　of　the　borate　bioglasses.　Moreover,　ab　initio　molecular　dynamics　simulations　have　been　utilized　to　elucidate　the　relationship　between　structural　alterations　and　in　vitro　bioactivity,　with　a　particular　emphasis　on　the　boron　coordination　number.　These　findings　provide　a　promising　strategy　for　the　development　of　borate　BGs　with　tailored　degradation　profiles　and　excellent　bioactivity,　making　them　as　strong　candidates　for　various　biomedical　applications.