Metal　organic　frameworks　(MOFs)　are　crystalline　compounds　composed　of　metal　ions　(or　metal　clusters)　and　organic　ligands.　Chiral　MOFs　have　been　successfully　utilized　as　no-　vel　materials　for　the　separation　of　chiral　enantiomers　by　chromatography,　demonstrating　excel-　lent　chiral　separation　performance.　In　this　study,　a　chiral　MOF-modified　silica　monolithic　capil-　lary　column　was　used　for　pressurized　capillary　electrochromatography.　First,　a　chiral　MOF　(Co-　glycyl-L-glutamic　acid,　Co-L-GG)　was　synthesized.　This　MOF　was　then　used　to　prepare　a　chiral　capillary　monolithic　column　via　a　one-step　in　situ　polymerization　method.　The　optimal　condi-　tions　for　preparing　the　chiral　capillary　monolithic　column　were　determined　as　follows:　Co-L-GG　amount,　5　mg;　polyethylene　glycol　amount,　0.　96　mg;　tetramethoxysilane　dosage,　3.　6　mL;　tri-　methoxymethylsilane　dosage,　0.　4　mL.　Next,　the　effects　of　the　separation　conditions　on　the　sep-　aration　of　chiral　drugs　were　investigated.　Under　the　conditions　of　an　applied　voltage　of　-20　kV　and　a　mobile　phase　consisting　of　acetonitrile　and　20　mmol/L　disodium　hydrogen　phosphate　(80　∶20,　v/v),　six　chiral　drugs　were　separated　within　3　min,　with　baseline　separation　achieved　for　amlodipine,　fluvastatin,　and　tryptophan.　Moreover,　the　prepared　chiral　capillary　monolithic　col-　umn　exhibited　good　reproducibility　and　stability.　Finally,　molecular　docking　studies　were　con-　ducted　using　AutoDock　to　explore　the　chiral　recognition　mechanism,　and　the　results　were　ana-　lyzed　using　Discovery　Studio.　The　results　indicated　that　larger　differences　in　binding　free　energy　between　Co-L-GG　and　the　enantiomers　of　the　chiral　drugs　were　correlated　with　higher　enantios-　electivity　factors.　However,　this　correlation　did　not　necessarily　lead　to　an　increase　in　resolution.　Co-L-GG,　which　is　enriched　with　primary　amines,　secondary　amines,　and　carbonyl　groups,　demonstrated　enantiomeric　recognition　capability.　In　conclusion,　this　study　demonstrates　that　chiral　MOFs　can　be　effectively　used　as　chiral　functional　monomers　to　prepare　chiral　monolithic　capillary　columns,　highlighting　their　significant　potential　for　the　separation　and　analysis　of　chiral　compounds.　The　comprehensive　exploration　of　the　synthesis,　characterization,　and　appli-　cations　of　these　MOFs　will　help　provide　valuable　insights　into　the　development　of　advanced　sep-　aration　technologies.　©　(2024),　(Zhongguo　Kexueyuan　-　Chinese　Academy　of　Sciences).　All　rights　reserved.