Sexual　desire　is　a　complex　construct　with　important　implications　for　sexual　functioning　and　well-being.　In　this　research,　we　translated　the　Sexual　Desire　Inventory　(SDI-2),　a　widely　used　scale　for　assessing　sexual　(desire),　into　25　languages　from　English　and　used　data　from　the　International　Sex　Survey　(ISS)　to　(a)　investigate　its　psychometric　properties　(i.e.　factorial　structure,　reliability,　validity,　and　measurement　invariance)　and　(b)　explore　the　expression　of　sexual　desire　across　different　countries,　genders,　and　sexual　orientations.　A　total　of　82,243　participants　from　42　countries　completed　the　SDI-2,　along　with　other　sexuality-related　scales.　Confirmatory　factor　analysis　supported　a　three-factor　solution　for　the　SDI-2　(CFI　=　.980;　RMSEA　=　.060),　encompassing　the　domains　of　＂Partner-related,＂　＂Attractive-person-related,＂　and　＂Solitary＂　sexual　desire.　The　reliability　of　the　total　score　and　subscales　were　excellent.　Likewise,　correlations　with　other　sexuality-related　variables　were　positive　yet　weak-to-moderate　in　effect　size.　Measurement　invariance　tests　supported　its　use　across　countries,　languages,　genders,　and　sexual　orientations.　Analysis　of　SDI-2　scores　according　to　these　variables　supported　its　ability　to　capture　group-based　differences　in　sexual　desire.　In　sum,　the　SDI-2　constitutes　a　psychometrically　robust　measure　for　the　assessment　of　sexual　desire　in　non-clinical　samples　with　utility　in　large-scale　cross-cultural　studies.