Femtosecond　laser　has　emerged　as　a　valuable　tool　for　the　fabrication　of　optical　fiber　sensors　with　novel　microstructures,　due　to　its　ultrashort　pulse　width　and　extremely　high　peak　intensity.　By　focusing　the　laser　beam　within　materials,　nonlinear　interaction　is　induced　within　the　focal　volume,　enabling　the　manufacture　of　unprecedented　microstructure　fibers.　In　this　review,　we　give　a　comprehensive　introduction　to　the　emerging　fiber　optic　biochemical　sensors　realized　by　femtosecond　laser　technology.　Firstly,　we　discuss　three　processing　schemes　and　their　corresponding　advantages　according　to　the　different　fabrication　effects,　which　involves　undeformative,　subtractive,　and　additive　processing.　Additionally,　benefited　from　adaptive　optics,　the　precision　and　flexibility　of　fiber　micro-fabrication　can　be　enhanced　by　the　multi-dimensional　dynamic　modulation　of　pulsed　beams.　Subsequently,　we　summarize　all-in-fiber　biochemical　sensors　fabricated　by　femtosecond　laser　in　terms　of　operation　principles,　device　structures,　fabrication　methods,　and　innovative　biochemical　functionalities,　respectively.　Finally,　we　evaluate　the　advantages　of　femtosecond　laser　processing　for　fiber　sensor　fabrication　over　existing　manufacturing　technologies,　and　discuss　the　challenges　and　potential　opportunities　for　biochemical　applications.