Bile　acids,　a　representative　diagnostic　indicator　of　liver　function,　are　used　to　visualize　the　extent　of　liver　injury.　Numerous　studies　have　shown　that　triclosan　(TCS)　exposure　leads　to　abnormal　bile　acid　metabolism.　As　a　result,　there　is　a　requirement　to　develop　a　fast　and　smart　means　to　quantitatively　monitor　abnormal　bile　acids　from　exposure　to　triclosan　in　bio-sample.　In　this　work,　solid-phase　microextraction　(SPME)　probes　of　sea　urchin-like　covalent　organic　frameworks　(COF)　were　in　situ　synthesized　on　steel　needles　by　using　1,3,5-tris(4-aminophenyl)　benzene　(TAPB)　and　2,5-dimethoxybenzene-1,4-dicarboxaldehyde　(DMTP)　as　two　organic　units　and　employed　for　extraction　of　bile　acids.　This　TAPB-DMTP-COF-SPME　possessed　an　excellent　specified　surface　area　(3351　m(2)　g(-1))　and　a　high　regular　porosity　(similar　to　3.6　nm),　which　was　an　ideal　adsorbent　to　concentrate　bile　acids　efficiently.　The　created　probe,　together　with　electrospray　ionization　mass　spectrometry　(ESI/MS),　proved　to　be　a　fast　and　specific　assay　for　the　detection　of　bile　acids　in　bio-samples.　The　proposed　method　had　a　low　limitation　of　detection　(0.03　mu　g　L-1),　good　linearity　(R-2　＞=　0.9931),　wide　linear　range　(0.10-1000.00　mu　g　L-1)　and　excellent　enrichment　factor　(63.60-252.00).　Based　on　these　excellent　properties,　it　was　successful　application　for　the　analyzing　of　bile　acids　in　mice　liver　and　feces,　demonstrating　the　great　potential　of　TAPB-DMTP-COF-SPME-ESI/MS　in　bile　acids　detection　and　liver　injury　diagnosis.