A　triple　signal　amplified　electrochemical　aptasensor　for　the　detection　of　bisphenol　A　(BPA)　was　developed　for　the　first　time　based　on　gold　nanoparticles　(AuNPs),　hemin/G-quadruplex　DNAzyme,　and　exonuclease　I　(Exo　I) assisted　amplification　strategies.　The　BPA　aptamer　(Apt)　hybridized　with　the　capture　probe　(CP)　was fixed　on　the　gold　electrode　(GE)　to　form　the　double-stranded　DNA　(dsDNA)　structure.　When　BPA　was　present,　the　Apt　was　detached　from　the　GE　surface　by　specific　recognition　between　the　BPA　and　Apt,　forming　BPA-Apt　complexes　in　solution.　The　complexes　could　be　selectively　digested　by　Exo　I,　releasing　BPA　to　participate　in　the　cycle　for　binding　to　other　Apt　in　dsDNA.　The　hybridization　of　the　CP　and　auxiliary　DNA　(aDNA)　within　the　detect　probe　DNA　(dpDNA)-AuNP-aDNA　nanocomplex　allowed　the　nanocomplex　to　connect　to　the　GE　surface.　The　dpDNA　interacted　with　K+　and　hemin　to　produce　hemin/G-quadruplex　DNAzyme,　which　catalyzed　H2O2　reduction,　accelerated　methylene　blue　(MB)　oxidization,　and　further　amplified　the　electrochemical　signal.　The　integration　of　triple　signal　amplification　strategies　with　aptamer-specific　recognition　enabled　sensitive　and　specific　detection　of　BPA.　Under　optimized　conditions,　the　aptasensor　exhibited　a　linear　range　of　0.1 pM–10 nM,　with　a　low　detection　limit　of　76　fM.　Moreover,　the　designed　aptasensor　was　successfully　applied　to　detect　BPA　in　lake　water,　fruit　juice,　and　honey　samples.　©　The　Author(s),　under　exclusive　licence　to　Springer-Verlag　GmbH　Austria,　part　of　Springer　Nature　2024.