Electrochemiluminescence　(ECL)　sensors　based　on　luminol　had　been　frequently　coupled　with　enzymatic　reactions　to　detect　various　targets.　However,　a　major　challenge　arises　from　the　conflict　between　the　neutral　environmental　required　for　enzymatic　activity　and　the　higher　luminescence　efficiency　of　luminol　under　alkaline　conditions.　In　this　study,　glucose　oxidase　(GOx)@Zn-HHTP　(HHTP　=　2,3,6,7,10,11-hexahydroxytriphenylene)　was　successfully　synthesized,　significantly　enhancing　the　stability　of　encapsulated　GOx.　At　pH　10.0,　the　enzyme　retained　80　%　of　its　catalytic　activity　compared　to　its　performance　under　neutral　conditions.　To　demonstrate　its　applicability,　GOx@Zn-HHTP　had　been　applied　to　construct　an　ECL　glucose　sensor.　Operating　under　alkaline　conditions,　this　sensor　maintained　a　high　luminescence　efficiency　of　luminol,　resulting　in　an　8.5-fold　increase　in　sensitivity　and　a　25-fold　reduction　in　the　detection　limit　compared　to　the　sensor　operating　under　neutral　environments.　This　sensor　was　successfully　applied　for　detection　of　glucose　in　sweat,　effectively　mitigating　interference　from　the　complex　matrix　of　sweat　samples　and　enabling　accurate　detection　of　glucose　at　low　concentrations.　©　2025　Elsevier　B.V.