The　development　of　anti-corrosion　and　anti-poison　electrocatalysts　for　both　the　hydrogen　oxidation　reaction　(HOR)　and　oxygen　reduction　reaction　(ORR)　is　of　great　importance　for　effective　applications　of　proton　exchange　membrane　fuel　cells　(PEMFCs).　In　this　study,　a　non-carbon　supported　catalyst,　Pt/TiO2-Ov,　enriched　with　oxygen　vacancies　(Ov),　is　successfully　synthesized　using　a　microwave-assisted　method.　This　catalyst　is　developed　as　a　bifunctional　electrocatalyst　with　superior　contamination　tolerance,　enabling　efficient　HOR　and　ORR　performance.　The　electronic　metal-support　interaction　(EMSI)　is　leveraged　to　facilitate　electron　transfer　between　Pt　and　Ti　atoms,　induced　by　the　formation　of　oxygen　vacancy　channels　in　the　small-sized,　high　surface　area　TiO2-Ov　support.　Notably,　TiO2-Ov　has　a　lower　bandgap　than　commercial　TiO2,　enhancing　its　catalytic　properties.　In　a　0.1　mol/L　HClO4　electrolyte,　the　normalized　Pt　mass　activity　(jk,m)　and　specific　activity　(j0,s)　of　Pt/TiO2-Ov　are　1.24　times　higher　than　those　of　commercial　Pt/C.　Furthermore,　Pt/TiO2-Ov　catalyst　exhibits　minimal　current　density　decay　after　a　prolonged　durability　testing　under　hydrogen　and　oxygen　atmospheres.　Remarkably,　under　a　H2/(1000×10−6)　CO　atmosphere,　the　relative　retention　rate　of　Pt/TiO2-Ov　significantly　exceeds　that　of　Pt/C　catalyst,　demonstrating　its　superior　CO　tolerance　and　promising　potential　for　practical　applications　in　PEMFCs.　This　study　highlights　the　critical　role　of　the　strong　metal-support　interaction　between　the　reducible　oxide　support　and　the　noble　metal　Pt　in　improving　long-term　performance　and　CO　poisoning　resistance.　©　Higher　Education　Press　2025.