Pathogens　pose　significant　threats　to　biosecurity　and　environmental　health　due　to　their　potential　for　widespread　outbreaks.　Effective　pathogen　detection　requires　methods　that　are　rapid,　sensitive,　specific,　and　informative.　Here,　we　proposed　a　multiplex　visual　detection　system　that　integrated　ultrafast　polymerase　chain　reaction　(PCR)　and　molecular　beacons,　allowing　the　simultaneous　detection　of　three　pathogens　in　a　one-pot　reaction.　The　ultrafast　PCR　protocol　employed　cycles　of　just　7 s　each,　allowing　the　entire　process-from　sampling　to　result-to　be　completed　within　only　10 min.　Molecular　beacons　hybridized　with　target　sequences　during　ultrafast　PCR,　generating　fluorescence　signals　that　are　visually　detectable　without　specialized　equipment.　Additionally,　we　developed　a　compact,　portable　cartridge　integrated　with　online　software　for　fluorescence　visualization　and　direct　result　output,　eliminating　the　need　for　bulky　instruments　and　specialized　personnel,　thereby　facilitating　point-of-care　testing　(POCT).　The　method　demonstrated　high　specificity　and　sensitivity,　with　a　limit　of　detection　(LOD)　as　low　as　23　copies　per　reaction.　It　achieved　a　100%　positive　detection　rate　in　practical　applications,　performing　comparably　to　standard　PCR.　Furthermore,　the　method　effectively　identified　low　concentrations　of　pathogens　in　animal　infection　samples.　This　ultrafast,　highly　sensitive,　specific,　and　informative　method　shows　significant　potential　for　POCT　applications,　including　food　safety　monitoring　and　clinical　diagnostics.　©　International　Human　Phenome　Institutes　(Shanghai)　2025.