Two-plex in vivo molecular imaging in the second near-infrared window for immunotherapeutic response - Details

author：

Indexed by：

Scopus

Abstract：

Tumor-infiltrating　CD8+　T　cells　and　programmed　death-1　(PD1)　levels　are　critical　indicators　for　tumor　immunophenotyping　and　therapeutic　decision-making.　Noninvasive　optical　imaging　in　the　second　near　infrared　window　(NIR-II)　is　particularly　well-suited　for　investigating　the　biological　processes　within　tumors　in　live　mammals,　thanks　to　its　deep-tissue　penetration　and　superior　spatiotemporal　resolution.　However,　in　vivo　NIR-II　imaging　has　primarily　been　restricted　to　a　single　probe　at　a　time.　Methods:　Herein,　we　developed　a　two-plex　NIR-II　molecular　imaging　method　utilizing　the　non-overlapping　fluorescence　emission　of　indocyanine　green　(ICG)　in　the　NIR-IIa　window　(1000-1200　nm)　and　PbS/CdS　core-shell　quantum　dots　(QDs)　in　the　NIR-IIb　window　(1500-1700　nm).　By　integrating　PD1　aptamer-labeled　ICG　(ICG-Apt-PD1,　targeting　PD1)　and　CD8　aptamer-labeled　QDs　(QDs@Apt-CD8,　targeting　CD8+　T　cells),　our　two-plex　NIR-II　molecular　imaging　enabled　simultaneous　and　noninvasive　monitoring　of　the　number　of　CD8+　T　cells　and　PD1　levels　in　tumors.　Results:　QDs@Apt-CD8　demonstrated　the　excellent　ability　for　in　vivo　imaging　of　tumor　infiltrating　CD8+　T　cells,　owing　to　its　strong　NIR-IIb　luminescence　and　the　high　selectivity　and　specificity.　This　two-plex　in　vivo　molecular　imaging　allowed　for　dynamic　monitoring　for　PD1　levels　and　the　number　of　CD8+　T　cells　in　tumors.　We　observed　the　heterogeneous　bio-distributions　of　PD1　and　CD8+　T　cells　across　different　tumor　types　and　revealed　the　tumor　immunophenotypes.　Moreover,　our　findings　indicated　that　the　low　PD1　and　high　CD8+　T　cells　levels　in　tumors　predicted　a　better　anti-tumor　effect.　Conclusions:　Such　in　vivo　noninvasive　NIR-II　molecular　imaging　would　complement　ex　vivo　biopsy-based　diagnostic　techniques,　and　it　could　contribute　to　developing　an　in　vivo　tumor　immune-scoring　algorithm　to　offer　a　more　precise　prediction　for　immunotherapeutic　response.　©　The　author(s).

Keyword：

immunotherapeutic response in vivo imaging programmed death-1 (PD1) second near-infrared window (NIR-II) tumor-infiltrating CD8+ T cells two-plex molecular imaging

Community：

[ 1 ] [Sun Y.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 2 ] [Sun Y.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 3 ] [Li R.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 4 ] [Li R.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, China
[ 5 ] [Cai Y.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 6 ] [Cai Y.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, China
[ 7 ] [Liu Y.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 8 ] [Liu Y.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, China
[ 9 ] [Wang P.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 10 ] [Wang P.]CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002, China
[ 11 ] [Wu M.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 12 ] [Wu M.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 13 ] [Zhang X.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 14 ] [Zhang X.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 15 ] [Liao N.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 16 ] [Zhang C.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 17 ] [Zhang C.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 18 ] [Zheng A.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 19 ] [Zheng A.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 20 ] [Xu H.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 21 ] [Zeng R.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 22 ] [Zeng Y.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 23 ] [Zeng Y.]Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China
[ 24 ] [Liu X.]The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 25 ] [Liu X.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 26 ] [Liu X.]CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002, China

Reprint 's Address：

Email：

Related Keywords：

Two-plex in vivo molecular imaging in the second near-infrared window for immunotherapeutic response
2025，THERANOSTICS
Dual activated NIR-II fluorescence and photoacoustic imaging-guided cancer chemo-radiotherapy using hybrid plasmonic-fluorescent assemblies
2020，NANO RESEARCH
Mesoporous radiosensitized nanoprobe for enhanced NIR-II photoacoustic imaging-guided accurate radio-chemotherapy
2022，NANO RESEARCH
Caveolin-mediated cytosolic delivery of spike nanoparticle enhances antitumor immunity of neoantigen vaccine for hepatocellular carcinoma
2023，THERANOSTICS
Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging
2025，NATURE COMMUNICATIONS

Type
Departments

All Years Choose Year From to