Pyroptosis,　often　referred　to　as　inflammatory　necrosis,　is　a　type　of　programmed　cell　death,　characterized　by　the　swelling　of　cells　until　the　cell　membranes　rupture,　resulting　in　the　release　of　intracellular　substances　and　a　strong　inflammatory　response.　Lipid　droplets　and　mitochondria　play　important　roles　in　cellular　activities.　A　strong　correlation　exists　between　pyroptosis　and　mitochondrial　dysfunction,　which　can　be　reflected　through　physiological　functions　and　involves　changes　in　the　mitochondrial　microenvironment　and　morphology.　In　this　work,　a　“double-responsive”　and　“dual-targeted”　fluorescent　probe　named　WD-2　was　constructed.　It　has　excellent　response　performance　to　viscosity　and　ONOO-,　and　can　simultaneously　monitor　the　relevant　levels　in　lipid　droplets　and　mitochondria.　Its　remarkable　targeting　ability　toward　mitochondria　and　lipid　droplets　has　been　verified　through　colocalization　experiments.　In　cell　imaging　experiments,　the　interaction　between　mitochondria　and　lipid　droplets　during　nutritional　stress　was　preliminarily　studied.　With　the　help　of　doxorubicin　hydrochloride,　the　changes　in　the　level　of　ONOO-　in　mitochondria　during　pyroptosis　were　explored,　providing　a　new　perspective　for　understanding　the　mechanism　of　this　process.　©　2025　American　Chemical　Society.