A　sustainable　solution　to　the　dramatic　spread　of　antibiotic　resistance　threatening　public　health　security　is　the　development　of　antibiotic-free　antimicrobial　substances.Inspired　by　natural　host　defense　mecha-nisms　involving　amino-terminal　copper-nickel　binding　motif(ATCUN)antimicrobial　peptides(AMPs),we　have　designed　and　prepared　an　artificial　complex(Cu@G-AMPs)incorporating　single-atom　Cu　cata-lysts　for　antibacterial　therapy.The　substrate　of　the　complex,formed　from　guanine　doped　with　abundant　heteroatoms,anchored　single　Cu　atoms　with　a　coordination　number　of　2　and　an　average　bond　length　of　1.91　A.Interestingly,Cu@G-AMPs,exhibiting　Fenton-like　catalytic　activity,caused　the　inactivation　of　methicillin-resistant　Staphylococcus　aureus(MRSA)by　generating　and　delivering　reactive　oxygen　species(ROS)cargo.Mechanistically,the　intrinsic　stress　response　system　of　MRSA　underwent　an　irreversible　col-lapse　when　Cu@G-AMPs　initiated　its　offensive　program　associated　with　non-specific　targets.Furthermore,Cu@G-AMPs,which　inherited　the　immunomodulatory　properties　of　AMPs,sequentially　car-ried　out　the　functions　of　pulling　edge　closure,stabilizing　granulation　tissue,promoting　collagen　fiber　pro-liferation,alleviating　inflammation,and　promoting　neovascularization　in　wound　areas　infected　by　MRSA.Our　results　show　that　Cu@G-AMPs　will　provide　a　new　perspective　on　untangling　the　complex　regulatory　networks　that　resistant　bacteria　have　cultivated　to　deactivate　commercial　antibiotics.