This　study　reports　the　synthesis　and　application　of　a　flower-like　chiral　metal-organic　framework　(CMOF)　based　on　copper　ions　and　tryptophan　(Cu(Trp)-CMOF).　The　Cu(Trp)-CMOF　was　successfully　synthesized　at　room　temperature　through　a　facile　method　with　hexadecyl　trimethyl　ammonium　bromide　(CTAB)　as　a　structuredirecting　agent.　The　material　exhibits　a　flower-like　morphology　with　hierarchical　porosity　and　well-defined　crystallinity　in　the　monoclinic　P21/c　space　group.　Comprehensive　characterization　revealed　that　different　optical　configurations　((R)-type,　(S)-type,　and　(RS)-type)　could　be　selectively　obtained　by　controlling　the　chirality　of　tryptophan　ligands.　The　synthesized　CMOFs　demonstrated　excellent　performance　in　two　key　applications:　colorimetric　recognition　of　amino　acid　enantiomers　and　separation　of　volatile　organic　compounds　(VOCs).　When　used　as　a　chromatographic　stationary　phase,　the　CMOF　significantly　enhanced　the　separation　of　challenging　structural　isomers,　particularly　improving　the　resolution　(RS)　of　xylene　isomers　(RS　from　1.03　to　2.62)　and　ethyltoluene　isomers　(RS　from　4.83　to　9.23)　compared　to　conventional　columns.　This　work　provides　new　insights　into　the　design　and　application　of　CMOFs　for　chiral　separation　and　recognition.