IL-17　(interleukin-17)　is　a　common　proinflammatory　cytokine　and　can　be　adopted　as　an　important　biomarker　for　several　diseases;　it　is　thus　very　necessary　to　design　an　effective　method　for　monitoring　IL-17.　Herein,　by　preparing　silver　nanoparticles/graphdiyne　(Ag/GDY)　with　simple　electroless　deposition　as　the　sensing　platform　and　methylene　blue/UiO-66-NH2　MOFs　(MB/MOFs)　as　the　signaling-amplifier,　a　highly　efficient　sandwich-like　electrochemical　immunosensing　(SLEI)　platform　was　designed　for　IL-17　detection.　The　superior　electrical　property,　easy　preparation,　large　surface　area,　and　high　stability　of　Ag/GDY　make　it　a　desirable　substrate　to　immobilize　primary　antibody　of　IL-17.　Meanwhile,　the　unique　structure　of　UiO-66-NH2　is　beneficial　to　immobilize　MB　probe　and　secondary　antibody.　By　optimizing　experimental　conditions,　the　MB　current　obtained　at　the　as-designed　SLEI　platform　increases　along　with　IL-17　increase,　and　the　related　linearity　is　1.0 pg mL−1–1.0 ng mL−1　coupled　with　a　low　detection　limit　of　0.3 pg mL−1.　It　is　expected　that　the　SLEI　platform　based　on　Ag/GDY　and　MB/MOFs　shows　some　potential　application　for　IL-17　detection.　©　The　Author(s),　under　exclusive　licence　to　Springer-Verlag　GmbH　Austria,　part　of　Springer　Nature　2025.