In　this　study,　we　present　the　innovative　design　and　comprehensive　evaluation　of　a　novel　point-of-care　testing　(POCT)　methodology　for　the　rapid　and　accurate　detection　of　T-2　toxin,　a　potent　mycotoxin　with　significant　implications　for　food　safety　and　human　health.　The　cornerstone　of　this　approach　lies　in　the　integration　of　a　copper-based　conductive　metal-organic　framework　(Cu3(HHTP)2)　with　a　target-responsive　DNA　hydrogel　system,　creating　a　dual-signal　readout　mechanism　that　significantly　enhances　detection　sensitivity　and　specificity.　Specifically,　the　hydrogel　covers　the　material＇s　surface　metal　active　sites,　which　become　exposed　upon　target　addition　due　to　hydrogel　collapse.　The　released　Cu3(HHTP)2　can　catalyze　the　oxidation　of　3,3′,5,5′-tetramethylbenzidine,　resulting　in　a　colorimetric　and　temperature　change　in　the　solution.　This　dual-mode　detection　strategy　enables　both　qualitative　assessment　through　direct　visual　inspection　of　color　change　and　quantitative　analysis　by　monitoring　the　solution　temperature　variation　post　laser　irradiation　with　a　thermometer.　Under　the　optimized　conditions,　the　detection　system　demonstrates　a　wide　range　spanning　from　5　to　200　ng/mL,　with　a　detection　limit　of　1.67　ng/mL.　This　method　has　been　successfully　demonstrated　through　the　analysis　of　real-world　samples,　yielding　encouraging　results　that　underscore　its　reliability　and　effectiveness.　The　proposed　dual-signal　approach　boasts　advantages　such　as　straightforward　operational　procedures,　unambiguous　signal　outputs,　and　robust　practicality,　making　it　an　attractive　option　for　mycotoxin　detection　in　resource-constrained　settings　and　developing　regions.　©　2025　Elsevier　B.V.