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author:

Wang, T. (Wang, T..) [1] | Jiang, W. (Jiang, W..) [2] | Huang, Z. (Huang, Z..) [3] | Yuan, Z. (Yuan, Z..) [4] | Chen, Z. (Chen, Z..) [5] | Lin, J. (Lin, J..) [6]

Indexed by:

Scopus

Abstract:

Acute respiratory infections, caused by RNA viruses like respiratory syncytial virus, influenza, rhinovirus, and coronavirus, are major global health threats. Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) is the gold standard for detecting these viruses but is time-consuming, complex, and requires specialized equipment. There is a need for rapid, convenient, and multi-target detection methods to improve disease prevention and control. This study developed a multi-target immunochromatographic detection method using LbuCas13a protein and “band elimination” test strips for detecting SARS-CoV-2 and influenza virus. The method’s performance was evaluated by testing known 5 positive and 4 negative samples for SARS-CoV-2 and comparing results with fluorescent PCR and colloidal gold methods. Detection sensitivity was quantified using digital PCR and qPCR. The immunochromatographic test strips showed 100% concordance with fluorescent PCR and colloidal gold methods in initial clinical SARS-CoV-2 detection. Subsequently, we used dual-target immunochromatographic test strips to detect 9 SARS-CoV-2 positive samples and 9 H3N2 positive samples. However, false negatives were observed in dual-target detection of SARS-CoV-2 and H3N2 samples, likely due to low sample concentration or sample degradation. The method had a minimum detection limit of 381.75 copies/µL, as determined by digital PCR and qPCR. The developed multi-target immunochromatographic detection method offers a rapid, low-cost, and simple approach for detecting both SARS-CoV-2 and influenza viruses. With high sensitivity, specificity, and reliability, this method holds promise as a practical tool for RNA virus diagnosis and improving public health response to respiratory infections. © The Author(s) 2025.

Keyword:

CRISPR-Cas13a Immunochromatographic test strip Multi-target detection Respiratory RNA virus

Community:

  • [ 1 ] [Wang T.]Institute of Applied Genomics, Fuzhou University, No.2 Xueyuan Road, Fuzhou, 350108, China
  • [ 2 ] [Wang T.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, China
  • [ 3 ] [Jiang W.]Institute of Applied Genomics, Fuzhou University, No.2 Xueyuan Road, Fuzhou, 350108, China
  • [ 4 ] [Jiang W.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, China
  • [ 5 ] [Huang Z.]Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, China
  • [ 6 ] [Yuan Z.]Institute of Applied Genomics, Fuzhou University, No.2 Xueyuan Road, Fuzhou, 350108, China
  • [ 7 ] [Yuan Z.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, China
  • [ 8 ] [Chen Z.]Fuzhou Center for Disease Control and Prevention, No. 199, Wansha Road, Zhanggang Street, Changle District, Fuzhou, 350209, China
  • [ 9 ] [Chen Z.]Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou, China
  • [ 10 ] [Lin J.]Institute of Applied Genomics, Fuzhou University, No.2 Xueyuan Road, Fuzhou, 350108, China
  • [ 11 ] [Lin J.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, China

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Source :

Virology Journal

ISSN: 1743-422X

Year: 2025

Issue: 1

Volume: 22

4 . 0 0 0

JCR@2023

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ESI Highly Cited Papers on the List: 0 Unfold All

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Chinese Cited Count:

30 Days PV: 0

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