Identification of novel ACE inhibitory peptides from Nannochloropsis oculata through peptidomics, in silico screening and molecular docking - Details

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Abstract：

The　protein　of　Nannochloropsis　oculata,　a　single-cell　marine　microalga,　was　hydrolyzed　by　different　enzymes.　The　low-molecular-weight　fraction　of　the　trypsin　hydrolysate　exhibited　the　best　angiotensin-converting　enzyme　inhibitory　(ACE–I)　activity　and　possessed　diverse　peptide　sequences.　After　in　silico　screening　and　in　vitro　verification,　three　novel　high-activity　peptides　were　identified.　Among　them,　GPGPFTVF　exhibits　the　lowest　IC50　value　(39.77　±　1.42　μM),　followed　by　PGPAIF　(52.03　±　2.19　μM)　and　WDPLGF　(107.96　±　4.84　μM).　Molecular　docking　shows　that　these　peptides　are　bound　to　ACE　via　the　residues　around　S1　and　S2　pockets　and　the　Zn2+　domain.　Hydrogen　bond　is　the　most　important　force　for　the　molecular　interaction,　and　all　three　peptides　inhibit　ACE　activity　via　competitive　inhibition　mode.　Further　simulated　gastrointestinal　digestion　illustrates　that　the　ACE-I　activity　is　stable　with　little　fluctuation.　This　study　reveals　that　N.　oculata　is　a　promising　source　for　ACE-I　peptide　not　only　because　of　its　sequence　diversity　but　also　its　high　activity.　©　2024

Keyword：

ACE inhibitory peptides Inhibition kinetics In silico screening Molecular docking Nannochloropsis oculata Simulated gastrointestinal digestion

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[ 1 ] [Lin L.]College of Chemical Engineering and Materials Science, Quanzhou Normal University, Fujian Province, Quanzhou, 362000, China
[ 2 ] [Yuan W.]Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, 362000, China
[ 3 ] [Xiao J.]Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, 362000, China
[ 4 ] [Xiao J.]School of Advanced Manufacturing, Fuzhou University, Quanzhou, 362251, China
[ 5 ] [Jia J.]SDIC Biotechnology Investment Co. LTD., State Development and Investment Corporation, Beijing, 100034, China
[ 6 ] [Xie Y.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China
[ 7 ] [Cai Q.]School of Pharmacy, Yancheng Teachers University, Yancheng, 224007, China
[ 8 ] [Dai C.]Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, 362000, China
[ 9 ] [Dai C.]Key Laboratory of Inshore Resources and Biotechnology Fujian Province University, Quanzhou, 362000, China
[ 10 ] [Li Q.]College of Chemical Engineering and Materials Science, Quanzhou Normal University, Fujian Province, Quanzhou, 362000, China
[ 11 ] [Wang B.]Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, 362000, China
[ 12 ] [Wang B.]Key Laboratory of Inshore Resources and Biotechnology Fujian Province University, Quanzhou, 362000, China

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Source ：

Food Chemistry

ISSN： 0308-8146

Year： 2025

Volume： 490

8 . 5 0 0

JCR@2023

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ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 3

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