An　important　environmental　issue　is　the　pollution　caused　by　poly(ethylene　terephthalate)　(PET)　and　the　emitted　monomers　terephthalic　acid　(TPA)　and　ethylene　glycol　(EG).　The　TPA　biodegradation　pathway　has　been　well　documented　for　the　microbial　biodegradation　and　assimilation　of　PET;　however,　the　PET　biodegradation　process　by　marine　microorganisms　via　the　EG　biodegradation　pathway　is　less　understood.　Here,　we　demonstrate　how　the　marine　bacteria　Pseudomonas　chengduensis　BC1815’s　membrane-anchored　PET　esterase　(OF113_10420)　breaks　down　PET.　We　also　explain　the　characteristics　of　this　enzyme　and　demonstrate　that　the　strain　assimilates　PET　via　the　EG　biodegradation　pathway.　In　addition　to　depolymerizing　PET,　PET　esterase　(OF113_10420)　hydrolyzes　BHET　and　MHET　to　generate　EG　more　efficiently　in　alkaline　circumstances.　It　has　been　discovered　that　P.　chengduensis　BC1815　cells　exhibiting　surface-displayed　PET　esterase　(OF113_10420)　have　the　potential　to　function　as　cell　biocatalysts　for　the　breakdown　of　PET.　Additionally,　we　look　into　the　microbial　communities　that　have　PET　esterase　linked　to　the　EG　biodegradation　pathway,　primarily　those　belonging　to　the　phyla　Proteobacteria　and　Actinobacteriota.　Consequently,　research　on　the　microbial　absorption　and　biodegradation　of　PET　via　the　EG　metabolism　route　increases　our　knowledge　of　the　bioremediation　of　PET　pollution　in　the　marine　environment　and　contributes　to　our　understanding　of　the　environmental　destiny　of　PET　pollutants　in　the　marine　environment.　©　2025　American　Chemical　Society.