Absolute　cross　sections　(ACSs)　are　needed　to　estimate　cellular　damage　induced　by　high-energy　radiation　(HER).　Low-energy　electrons　(LEEs),　which　are　the　most　numerous　secondary　particles　generated　by　HER,　can　trigger　hyperthermal　reactions　in　DNA.　ACSs　for　such　reactions　are　essential　input　parameters　to　calculate　radiobiological　effectiveness,　particularly　in　targeted　radiotherapy.　Using　a　mathematical　model,　we　generate　ACSs　from　effective　damage　yields　induced　by　LEE　impact　on　3197　base-pair　plasmid　DNA　films.　Direct　or　enzyme-revealed　conformational　damages,　quantified　by　electrophoresis,　provide　the　first　complete　set　of　ACSs　for　inducing　crosslinks,　double-strand　breaks　(DSBs),　single-strand　breaks,　base-damage-related　crosslinks,　non-DSB　clustered　damages　(NDCDs),　and　isolated　base　damages.　These　ACSs　are　generated　across　the　1–20　eV　range,　at　one　eV　intervals.　They　exhibit　a　strong　energy　dependence　with　maximum　values　at　10　eV　of　3.7　±　0.8,　3.5　±　0.6,　45.4　±　4.1,　2.9　±　1.1,　5.1　±　1.4,　and　54.0　±　16.4　×　10−15　cm2,　respectively.　ACSs　for　DSBs,　NDCDs,　and　crosslinks　clearly　indicate　that　lesions　threatening　cell　function　and　genetic　stability　can　be　generated　by　a　single　LEE.　At　5　and　10　eV,　total　damage　ACSs　are　63%　and　80%　larger,　respectively,　than　those　previously　determined　for　the　same　plasmids　bound　to　arginine,　a　constituent　of　histones　protecting　DNA.　©　2025　by　the　authors.