Background:Colorectal　cancer(CRC)　is　a　significant　global　health　concern,characterized　by　complex　interactions　involving　genetic,epigenetic,and　environmental　factors.Mitochondrial　metabolic　reprogramming　is　increasingly　recognized　as　a　critical　hallmark　of　cancer　progression,with　SIRT5,a　key　mitochondrial　deacetylase　belonging　to　the　sirtuin　family,playing　pivotal　roles　in　modulating　mitochondrial　function,metabolism,and　oxidative　stress　responses.Despite　growing　evidence　of　SIRT5＇s　involvement　in　various　cancers,its　specific　role　and　mechanisms　in　CRC　remain　inadequately　understood.This　study　aimed　to　elucidate　the　function　of　SIRT5　in　colorectal　cancer　through　an　integrated　bioinformatics　and　experimental　approach.Methods:We　utilized　publicly　available　CRC　datasets　from　TCGA　and　GEO　to　perform　comprehensive　bioinformatics　analyses,including　Weighted　Gene　Co-expression　Network　Analysis(WGCNA),LASSO　regression　modeling,and　gene　set　enrichment　analysis(GSEA).Subsequently,utilizing　colorectal　cancer　tissue　sect...