Two-dimensional　conductive　metal-organic　frameworks　(2D　c-MOFs)　hold　great　promise　for　electrochemical　applications　due　to　their　pi-conjugated　structures　and　high　conductivity,　but　their　application　in　electrochemiluminescence　(ECL)　sensing　remains　largely　unexplored.　In　this　study,　we　synthesized　2D　c-MOF　Cu3(benzenehexathiol)　nanosheets　(CuBHT　NSs)　with　lateral　dimensions　of　2-10　mu　m　and　a　thickness　of　24　nm　and　immobilized　them　on　indium　tin　oxide　(ITO)　electrodes.　This　platform　facilitated　the　first　study　of　luminol＇s　cathodic　ECL　behavior.　CuBHT　NSs　exhibited　exceptional　oxygen　reduction　reaction　(ORR)　catalytic　activity,　significantly　enhancing　the　generation　of　reactive　oxygen　species　(ROS)　and　thereby　increasing　the　luminol＇s　cathodic　ECL　response.　Mechanistic　studies　confirmed　that　unsaturated　Cu　sites　in　CuBHT　play　a　crucial　role,　as　Uric　acid　(UA)　can　bind　to　unsaturated　Cu　sites,　inhibiting　ROS　production.　This　outcome　enabled　a　highly　sensitive　and　selective　ECL　sensor　for　UA　with　a　wide　linear　range　(10　nM-0.1　mM)　and　a　low　detection　limit　(3.5　nM,　S/N　=　3).　The　sensor　was　successfully　applied　to　detect　UA　in　serum　samples.