Enhancing　luminol-based　chemiluminescence　(CL)　emission　through　functionalized　materials　is　essential　for　developing　highly　sensitive　method　in　rapid　analysis.　Herein,　a　Fe-MIL-101(NH2)　enhanced　CL　method　was　developed　for　the　selective　determination　of　glutamic　acid　(Glu)　in　serum.　By　utilizing　Fe-MIL-101(NH2)　as　an　effective　catalyst　for　the　first　time,　the　CL　intensity　of　the　luminol-H2O2　system　was　amplified,　and　this　system　exhibited　a　selective　response　to　Glu.　Mechanism　studies　demonstrated　that　Fe-MIL-101(NH2)　selectively　recognizes　HOO−　and　Glu　through　host-guest　interactions,　thereby　accelerating　the　luminol-H2O2　reaction　kinetics.　Under　optimal　conditions,　the　CL　intensity　exhibited　a　linear　relationship　with　the　concentration　of　Glu　in　the　range　of　0.01–10　μg/mL,　with　the　limited　of　detection　(LOD)　of　0.003　μg/mL.　This　method　was　successfully　applied　to　determine　Glu　in　human　serum　from　epilepsy　patients　and　healthy　individuals,　with　results　that　consistent　with　those　from　high-performance　liquid　chromatography-mass　spectrometry　(HPLC-MS).　New　applications　were　opened　up　for　CL　and　provided　a　rapid　and　reliable　alternative　method　for　diagnosing　mental　disorders.　©　2025　Elsevier　B.V.