Oxidative　stress　and　ferroptosis　are　important　processes　linked　to　the　development　of　neurodegenerative　diseases　and　may　represent　significant　therapeutic　targets　for　conditions　like　cancer.　Additionally,　the　relationship　between　mitochondria　and　lysosomes　plays　a　crucial　role　in　important　physiological　functions,　such　as　ferroptosis　and　oxidative　stress.　This　study　developed　a　mitochondrial-targeting,　viscosity-sensitive　fluorescent　probe,　NI-QNL,　utilizing　the　TICT　mechanism,　intended　for　the　quick　detection　and　observation　of　viscosity　changes　and　the　interactions　between　mitochondria　and　lysosomes　during　ferroptosis　caused　by　oxidative　stress.　Furthermore,　a　Parkinson＇s　disease　model　was　established　to　explore　the　application　potential　of　NI-QNL　in　disease　diagnosis.　The　probe　NI-QNL　offers　advantages　such　as　a　large　Stokes　shift,　good　selectivity,　and　high　sensitivity,　allowing　for　quick　responses　to　viscosity　changes.　Specifically,　when　NI-QNL　is　in　PBS　or　a　low-viscosity　system,　the　molecules　can　rotate　freely　due　to　the　TICT　effect,　leading　to　weak　fluorescence.　However,　in　a　high-viscosity　environment,　the　TICT　effect　of　NI-QNL　is　suppressed,　releasing　a　strong　fluorescence　signal,　thus　enabling　sensitive　viscosity　detection.　Notably,　NI-QNL　achieved　efficient　dynamic　monitoring　of　cellular　viscosity　and　further　tracked　the　dynamic　viscosity　changes　during　cellular　ferroptosis　in　real　time　via　various　induction　methods.　Additionally,　it　monitored　the　interactions　between　mitochondria　and　lysosomes　during　these　processes.　Finally,　using　a　zebrafish　model,　the　early　diagnostic　capability　of　NI-QNL　for　Parkinson＇s　disease　was　investigated,　along　with　an　assessment　of　the　therapeutic　effects　of　curcumin　on　Parkinson＇s　disease.　©　2025　Elsevier　B.V.