To　simplify　modeling,　the　nanofluid　concentration　distribution　inside　the　tumor　was　generally　presented　in　the　form　of　a　circular　magnetic　nanoparticles　(MNPs)　core　or　a　given　ideal　shape,　but　not　a　more　actual　situation　by　injection　behavior　modeling　in　previous　studies.　This　study　first　establishes　the　nanofluid　injection　models　with　and　without　backflow　before　therapy　in　order　to　obtain　the　corresponding　concentration　distribution　within　the　malignant　region　and　then　explores　the　impact　of　different　injection　behaviors　on　both　therapeutic　temperature　and　heat-induced　damage　for　biological　tissue　during　magnetic　nanofluid　hyperthermia.　Furthermore,　the　influence　of　diffusion　behavior　after　the　nanofluid　injection　on　the　therapeutic　effect　is　also　taken　into　consideration,　and　the　impact　of　this　behavior　on　hyperthermia　will　also　be　investigated　in　this　study.　The　analysis　results　reveal　that　the　injection　model　with　backflow　can　not　only　lead　to　a　better　magnetic　fluid　distribution　but　also　a　better　therapeutic　temperature　and　heat-induced　damage　for　biological　tissue　with　respect　to　the　ideal　case.　In　addition,　the　prolonged　diffusion　after　the　nanofluid　injection　can　effectively　improve　the　therapeutic　effect　after　combining　a　proper　heat　generation　for　MNPs　during　magnetic　hyperthermia.　©　2025　Author(s).