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学者姓名:李小晶

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Determination of three ephedrine psychoactive substances in sewage using solid-phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry SCIE
期刊论文 | 2024 , 38 (20) | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
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RationaleIn recent years, ephedrine psychoactive substances have attracted much attention due to their prevalence in water bodies and potential threat to aquatic ecosystems. Psychoactive substances have been considered as a new type of environmental pollutant due to their unpredictable potential risks to the behavior and nervous system of non-target organisms. A rapid, sensitive, selective, and robust method for the quantification of three ephedrine psychoactive substances in sewage is needed.MethodsAn ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of three ephedrine psychoactive substances in water. The optimal processing conditions were determined by optimizing the chromatography-mass spectrometry and solid-phase extraction (SPE) conditions (e.g., the SPE column, sample pH, washing, and elution), and the treatment conditions were determined; this was achieved via positive ion scanning in multiple reaction monitoring mode. Poly-Sery MCX was selected as the extraction column, with samples loaded at pH 3. And 4-mL solution of 2% formic acid (FA) aqueous solution was used as the eluent; the target compounds were eluted with 5 mL of 5% NH4OH in acetonitrile (ACN) solution. The best results were obtained when the residue was resolubulization in ACN after nitrogen evaporation.ResultsThe developed UPLC-MS/MS showed a good linear relationship in the range of 0-50.00 mu g/L, with determination coefficients (R2) greater than 0.9990. The detection limit and quantitation limit were 0.05-0.10 and 0.20-0.50 mu g/L, respectively. Recovery rates of the target compounds in blank sewage at three different concentrations ranged from 92.37% to 106.31%, with relative standard deviations (RSDs) of 0.77%-4.83% (n = 7).ConclusionsThis method has been successfully applied to the analysis of surface water and domestic sewage, and the samples were processed stably, indicating that the method is practical for the determination of ephedrine psychoactive drugs in water bodies.

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GB/T 7714 Xu, Yiqin , Yang, Fang , Ye, Hong et al. Determination of three ephedrine psychoactive substances in sewage using solid-phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry [J]. | RAPID COMMUNICATIONS IN MASS SPECTROMETRY , 2024 , 38 (20) .
MLA Xu, Yiqin et al. "Determination of three ephedrine psychoactive substances in sewage using solid-phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry" . | RAPID COMMUNICATIONS IN MASS SPECTROMETRY 38 . 20 (2024) .
APA Xu, Yiqin , Yang, Fang , Ye, Hong , Tang, Qingqiang , Chen, Yanwen , Gao, Zhigang et al. Determination of three ephedrine psychoactive substances in sewage using solid-phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry . | RAPID COMMUNICATIONS IN MASS SPECTROMETRY , 2024 , 38 (20) .
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Nitrogen-doped cobalt oxide via atmospheric modulation activates PMS for rapid degradation of organic pollutants: Role of nitrogen fraction on sulfapyridine elimination SCIE
期刊论文 | 2024 , 67 | JOURNAL OF WATER PROCESS ENGINEERING
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The study synthesized nitrogen-doped cobalt oxide precursors using a one-pot hydrothermal method. Three catalysts, N&CoO-Ar, N&CoO-N, and N&CoO-NH, were obtained by calcination under different atmospheric environments (argon, nitrogen, and nitrogen-hydrogen mixture). Characterization results showed that all the prepared materials consisted primarily of carbon components and cobalt oxide, while the catalysts calcined in a nitrogen-hydrogen atmosphere owned the highest content of pyridine nitrogen. Degradation experiments on sulfapyridine (SPD) showed that the N&CoO-NH/PMS system exhibited the best SPD degradation performance (the apparent rate constants were 3.0, 5.1 and 8.3 times larger those of N&CoO-N/PMS, N&CoO-Ar/PMS, and pure CoO/PMS), and was able to rapidly degrade the pollutant within 5 min. The quenching experiments and electrochemical experiments indicated that the nitrogen component modulation improved the chemisorption between PMS and N&CoO-NH and enhanced the electron transfer efficiency between N&CoO-NH/PMS and the pollutant. In addition, the N&CoO-NH/PMS system exhibited good stability and a wide pH applicability. Furthermore, N&CoO-NH/PMS also exhibited good anti-interference performance in the presence of Cl-, HCO3-, NO3-, PO42-, CO32-, and HA, as well as excellent feasibility of treating actual wastewater.

Keyword :

Cobalt-based catalysts Cobalt-based catalysts Degradation of organic pollutants Degradation of organic pollutants DFT DFT N doping N doping Permonosulfate activation Permonosulfate activation

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GB/T 7714 Zou, Lingxiao , Yang, Kai , Hu, Yihui et al. Nitrogen-doped cobalt oxide via atmospheric modulation activates PMS for rapid degradation of organic pollutants: Role of nitrogen fraction on sulfapyridine elimination [J]. | JOURNAL OF WATER PROCESS ENGINEERING , 2024 , 67 .
MLA Zou, Lingxiao et al. "Nitrogen-doped cobalt oxide via atmospheric modulation activates PMS for rapid degradation of organic pollutants: Role of nitrogen fraction on sulfapyridine elimination" . | JOURNAL OF WATER PROCESS ENGINEERING 67 (2024) .
APA Zou, Lingxiao , Yang, Kai , Hu, Yihui , Guo, Xingyu , Li, Xiaojing , Lv, Yuancai et al. Nitrogen-doped cobalt oxide via atmospheric modulation activates PMS for rapid degradation of organic pollutants: Role of nitrogen fraction on sulfapyridine elimination . | JOURNAL OF WATER PROCESS ENGINEERING , 2024 , 67 .
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Efficient capture of fluoroquinolones in urine and milk samples with multi-monolith fibers solid phase microextraction based on hybrid metal-organic framework/monolith material SCIE
期刊论文 | 2023 , 189 | MICROCHEMICAL JOURNAL
WoS CC Cited Count: 7
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To capture fluoroquinolones (FQs) from complex samples efficiently, a new hybrid metal-organic framework (MOF)@monolith material (HMM) was fabricated and employed as the extraction medium of multi-monolith fibers solid phase microextraction (MMF-SPME). Porous monolithic fibers based on allyltrimethylammonium chloride-co-ethylene glycol dimethacrylate/divinylbenzene polymer were fabricated firstly. After that, the strategy of layer-by-layer self-assembly was employed to prepare zeolitic imidazolate frameworks within the pores and the surface of the monolith. Various characterization results well evidenced that the introduction of MOF not only enhanced the surface area of adsorbent, but also introduced new active sites. The prepared HMM/ MMF-SPME presented satisfactory capture performance towards studied FQs by multiple interactions. Enrich-ment factors of FQs in human urine and milk samples were in the ranges of 99-128 and 85-121, respectively. Under the most beneficial fabrication and extraction parameters, HMM/MMF-SPME was combined with high-performance liquid chromatography to quantify trace FQs in urine and milk samples. High sensitivity (limits of detection were 0.0072-0.031 mu g/L for urine sample and 0.016-0.057 mu g/kg for milk sample) and good pre-cision (RSDs below 10%) were obtained. In the analysis of FQs in actual samples, the recovery at different spiked contents varied from 77.4% to 120%, with good reproducibility. Accuracy and reliability of established approach were well verified with confirmation experiments. Satisfactory results evidence that the developed HMM/MMF-SPME can be a prominent technique for the capture of trace FQs in complex samples.

Keyword :

Adsorbent Adsorbent Fluoroquinolones Fluoroquinolones Metal-organic framework Metal-organic framework Monolith Monolith Solid phase microextraction phase Solid phase microextraction phase

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GB/T 7714 Chen, Hexun , Wu, Jiangyi , Xiong, Qingbiao et al. Efficient capture of fluoroquinolones in urine and milk samples with multi-monolith fibers solid phase microextraction based on hybrid metal-organic framework/monolith material [J]. | MICROCHEMICAL JOURNAL , 2023 , 189 .
MLA Chen, Hexun et al. "Efficient capture of fluoroquinolones in urine and milk samples with multi-monolith fibers solid phase microextraction based on hybrid metal-organic framework/monolith material" . | MICROCHEMICAL JOURNAL 189 (2023) .
APA Chen, Hexun , Wu, Jiangyi , Xiong, Qingbiao , Li, Xiaojing , Huang, Xiaojia . Efficient capture of fluoroquinolones in urine and milk samples with multi-monolith fibers solid phase microextraction based on hybrid metal-organic framework/monolith material . | MICROCHEMICAL JOURNAL , 2023 , 189 .
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Adoption of new strategy for molecularly imprinted polymer based in-tube solid phase microextraction to improve specific recognition performance and extraction efficiency SCIE
期刊论文 | 2023 , 194 | MICROCHEMICAL JOURNAL
WoS CC Cited Count: 6
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In-tube solid phase microextraction (IT-SPME) utilizing molecularly imprinted polymers (MIPs) as extraction phase has received wide attention due to various merits. However, low extraction efficiency and unsatisfactory recognition performance have limited its extensive application. To circumvent the limitations, a new strategy that implementation of magnetic fields during extraction stage was introduced to improve the extraction efficiency and recognition performance of MIPs-based IT-SPME. Firstly, using 2,4-dinitroaniline (2,4-DNA) as model template, a MIP mingled with magnetic nanoparticles (Fe3O4) was in-situ prepared in a capillary and employed as the microextraction column of IT-SPME. In the second step, a magnetic coil was wrapped the as-prepared MIPbased microextraction column so as to produce variable magnetic fields in extraction step. Results revealed that the application of magnetic field during the adsorption step improved the specific extraction performance towards template. The extraction efficiency and imprinting factor towards 2,4-DNA increased from 58% and 2.5 to 81% and 3.1, respectively. Practicality of the introduced magnetism-assisted IT-SPME technique based on MIP was demonstrated by on-line hyphenating with HPLC/DAD to quantify trace 2,4-DNA in a variety of environmental waters. High sensitivity (limit of detection was 0.060 mu g/L), good precision (RSDs were below 10%) and satisfactory recoveries (82.1-111%) were obtained. Furthermore, the possible selective extraction mechanism under magnetic fields was deduced.

Keyword :

4-dinitroaniline 4-dinitroaniline In-tube solid phase microextraction In-tube solid phase microextraction Magnetic fiel d Magnetic fiel d Molecularly imprinted polymer Molecularly imprinted polymer Water Water

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GB/T 7714 Song, Xiaochong , Li, Xiaojing , Wang, Jingjuan et al. Adoption of new strategy for molecularly imprinted polymer based in-tube solid phase microextraction to improve specific recognition performance and extraction efficiency [J]. | MICROCHEMICAL JOURNAL , 2023 , 194 .
MLA Song, Xiaochong et al. "Adoption of new strategy for molecularly imprinted polymer based in-tube solid phase microextraction to improve specific recognition performance and extraction efficiency" . | MICROCHEMICAL JOURNAL 194 (2023) .
APA Song, Xiaochong , Li, Xiaojing , Wang, Jingjuan , Huang, Xiaojia . Adoption of new strategy for molecularly imprinted polymer based in-tube solid phase microextraction to improve specific recognition performance and extraction efficiency . | MICROCHEMICAL JOURNAL , 2023 , 194 .
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Online measurement of tetraethyllead in aqueous samples utilizing monolith-base d magnetism-enhance d in-tub e solid phase microextraction coupled with chromatographic analysis SCIE
期刊论文 | 2023 , 1700 | JOURNAL OF CHROMATOGRAPHY A
WoS CC Cited Count: 1
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A new procedure that utilizing a preconcentration system based on magnetism-enhanced in-tube solid phase microextraction (ME/IT-SPME) and detection by HPLC with diode array detector (DAD) after liq-uid desorption from the microextraction column has been developed for the online measurement of tetraethyllead (TEL) in various aqueous samples. In this connection, according to the chemical features of TEL, porous monolith mingled with Fe3O4 nanoparticles were designed and synthesized in a silica cap-illary, and used as the microextraction column of ME/IT-SPME. To favor the implement of variable mag-netic fields during extraction procedure, the as-prepared microextraction column was twined a magnetic coil. Results revealed that the exertion of magnetic field during the adsorption and eluting procedures assisted the extraction of TEL with an enhancement by 52% in extraction efficiency. Under the most ben-eficial conditions, the developed ME/IT-SPME was online hyphenated with HPLC/DAD to measure trace TEL in various aqueous samples. The limit of detection was 0.082 mu g/L and the RSDs for precision were in the range of 6.3-8.5%. The recoveries with low, medium and high fortified levels varied from 80.6% to 95.0% with good repeatability. To the best of knowledge, this is the first study that using IT-SPME to extract TEL and then online quantification with HPLC/DAD.(c) 2023 Published by Elsevier B.V.

Keyword :

Aqueous samples Aqueous samples HPLC HPLC Magnetism-enhanced in -tube solid phase Magnetism-enhanced in -tube solid phase microextraction microextraction Monolith Monolith Tetraethyllead Tetraethyllead

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GB/T 7714 Song, Xiaochong , Meng, Xin , Chen, Minshi et al. Online measurement of tetraethyllead in aqueous samples utilizing monolith-base d magnetism-enhance d in-tub e solid phase microextraction coupled with chromatographic analysis [J]. | JOURNAL OF CHROMATOGRAPHY A , 2023 , 1700 .
MLA Song, Xiaochong et al. "Online measurement of tetraethyllead in aqueous samples utilizing monolith-base d magnetism-enhance d in-tub e solid phase microextraction coupled with chromatographic analysis" . | JOURNAL OF CHROMATOGRAPHY A 1700 (2023) .
APA Song, Xiaochong , Meng, Xin , Chen, Minshi , Wang, Lei , Li, Xiaojing , Huang, Xiaojia . Online measurement of tetraethyllead in aqueous samples utilizing monolith-base d magnetism-enhance d in-tub e solid phase microextraction coupled with chromatographic analysis . | JOURNAL OF CHROMATOGRAPHY A , 2023 , 1700 .
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Quantification analysis of microplastics released from disposable polystyrene tableware with fluorescent polymer staining SCIE
期刊论文 | 2022 , 864 | SCIENCE OF THE TOTAL ENVIRONMENT
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Ingesting microplastics (MPs) from plastic tableware is an important source of health risk to human bodies. However, the comprehensive information of MPs released from disposable tableware has not been explored. Herein, a new visual quantification method for polystyrene MPs is proposed with carbon nitride fluorescent polymers staining, which can overcome the disadvantages of high signal background and photobleaching derived from organic dyes staining. Combining with fluorescence microscope and ImageJ software, the quantity, shape, and size distribution of MPs carried by the brand-new disposable polystyrene tableware (DPT) samples before usage and released from the clean DPT samples in different simulated usage scenes were studied. The brand-new DPT samples were found to carry a large number of MPs particles and the clean DPT samples could release MPs during usage. Fiber and fragment are the main morphology of the detected MPs and fiber accounts for 45-52 %. The particles with size <50 mu m are the majority of the detected MPs and the distribution fraction of MPs particles is gradually decreased with the raising of particle size within 50 mu m. The released MPs particles are increased with the raising of contact time and temperature, and greatly boosted for the DPT samples with cracks. The DPT samples are more like to release MPs in weak acidic condition (pH 4.0) than in weak alkaline (pH 8.3) and neutral (pH 7.0) conditions. The obtained results help to assess the food safety of tack-out food and the health risk of MPs exposure to human.

Keyword :

Carbon nitride Carbon nitride Disposable tableware Disposable tableware Fluorescent imaging Fluorescent imaging Microplastics Microplastics Polystyrene Polystyrene

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GB/T 7714 Zheng, Xueyi , Feng, Qiaocheng , Chen, Jingru et al. Quantification analysis of microplastics released from disposable polystyrene tableware with fluorescent polymer staining [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 864 .
MLA Zheng, Xueyi et al. "Quantification analysis of microplastics released from disposable polystyrene tableware with fluorescent polymer staining" . | SCIENCE OF THE TOTAL ENVIRONMENT 864 (2022) .
APA Zheng, Xueyi , Feng, Qiaocheng , Chen, Jingru , Yan, Jiaquan , Li, Xiaojing , Guo, Liangqia . Quantification analysis of microplastics released from disposable polystyrene tableware with fluorescent polymer staining . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 864 .
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Identification of the age of white tea using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) coupled with multivariate analysis SCIE
期刊论文 | 2022 , 36 (3) | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
WoS CC Cited Count: 4
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Rationale In recent years, white tea has become increasingly popular. Some merchants confuse the age of white tea and sell poor-quality products for profit. Therefore, it is necessary to provide technical support for product authentication and valorization in white tea of different marked ages. Methods Volatile organic compounds (VOCs) were detected by proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) and identified as volatile fingerprints. PTR-TOF-MS combined with multivariate analysis was found to identify white tea of four different marked ages (1, 3, 5, and 8 years) for authentication. Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used as classification models to identify key volatile metabolites. Results The OPLS-DA model achieved the best results (96.67%, 96.67%, 96.67%, and 96.67% in the training set and 96.00%, 96.00%, 100%, and 100% in the prediction set for 1-year, 3-year, 5-year, and 8-year tea samples, respectively), showing that PTR-TOF-MS with the OPLS-DA model could successfully be used in the identification of white tea with different marked ages. Out of the 60 identified VOCs, 26 volatile materials were closely correlated with tea age and were used as markers to discriminate white tea of different ages. Conclusions PTR-TOF-MS coupled with multivariate analysis could be applied for quality evaluation of tea products of different ages and provided a feasible technical support for product authentication and valorization in white tea of different marked ages.

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GB/T 7714 Wu, Weihua , Zhang, Dandan , He, Ye et al. Identification of the age of white tea using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) coupled with multivariate analysis [J]. | RAPID COMMUNICATIONS IN MASS SPECTROMETRY , 2022 , 36 (3) .
MLA Wu, Weihua et al. "Identification of the age of white tea using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) coupled with multivariate analysis" . | RAPID COMMUNICATIONS IN MASS SPECTROMETRY 36 . 3 (2022) .
APA Wu, Weihua , Zhang, Dandan , He, Ye , Cao, Jie , Li, Xiaojing . Identification of the age of white tea using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) coupled with multivariate analysis . | RAPID COMMUNICATIONS IN MASS SPECTROMETRY , 2022 , 36 (3) .
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At-line coupling of UPLC to chip-electrospray-FTICR-MS SCIE
期刊论文 | 2007 , 389 (5) , 1439-1446 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
WoS CC Cited Count: 16
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Since highly sensitive on-line coupling of UPLC with FTICR-MS is technically infeasible due to their different scan rates, at-line coupling of these techniques was developed for rapid analysis. To enable cutting of one peak of the chromatogram into one fraction, several conditions and relationships were investigated, e.g. the optimum volume of the inserted delay loop, the relationship between retention time, loop outlet drop speed, individual drop volume versus mobile phase composition under constant speed, and linear solvent strength gradient elution modes. Good and reproducible results were achieved applying UPLC as an efficient separation and fast fractionation tool before the FTICR-MS measurements. A chip-based nanoelectrospray ionization system was employed which was perfectly suited to handling the small-volume fractions and was thus chosen for the at-line coupling. The method was initially applied to spiked extracts of cell-free bacterial culture supernatants in which bacterial signalling compounds, namely N-acyl homoserine lactones (AHL), were detected. Good reproducibility and high recovery was observed. Afterwards, a culture supernatant of Erwinia sp. JX3.2, a putative AHL producer, was investigated and N-hexanoyl-homoserine lactone was determined as a possible signalling molecule. More reliable assignments were achieved by use of at-line coupling of UPLC and FTICR-MS compared with off-line measurements.

Keyword :

at-line coupling at-line coupling fractionation fractionation FTICR-MS FTICR-MS N-Acylhomoserine lactones N-Acylhomoserine lactones UPLC UPLC

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GB/T 7714 Li, Xiaojing , Fekete, Agnes , Englmann, Matthias et al. At-line coupling of UPLC to chip-electrospray-FTICR-MS [J]. | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2007 , 389 (5) : 1439-1446 .
MLA Li, Xiaojing et al. "At-line coupling of UPLC to chip-electrospray-FTICR-MS" . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY 389 . 5 (2007) : 1439-1446 .
APA Li, Xiaojing , Fekete, Agnes , Englmann, Matthias , Frommberger, Moritz , Lv, Shuiyuan , Chen, Guonan et al. At-line coupling of UPLC to chip-electrospray-FTICR-MS . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2007 , 389 (5) , 1439-1446 .
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The hydrolysis of unsubstituted N-acylhomoserine lactones to their homoserine metabolites Analytical approaches using ultra performance liquid chromatography SCIE
期刊论文 | 2007 , 1160 (1-2) , 184-193 | JOURNAL OF CHROMATOGRAPHY A
WoS CC Cited Count: 34
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Derivatives of N-acylhomoserine lactones (HSLs) with different alkanoyl side chains occur as quorum or diffusion sensing molecules in Gram-negative bacteria and their quantitative chemical analysis became important as a possible way to follow regulation processes of their pathogenicity towards plants and animals. The lactone-ring of HSLs is chemically and biologically not stable: the corresponding serines can be formed in alkaline conditions and these may presumably behave inactive for the biological system. A fast and MS compatible liquid chromatographic method applying high pressure (ultra performance liquid chromatography) with diode array detection was optimized for the rapid quantitative determination of HSLs and their corresponding hydrolysis products. The technique was used to follow and model the hydrolysis reactions of HSLs as function of pH under controlled conditions. Moreover, the method could be triggered to allow a confirmation in the assignment of the potential HSLs in real samples by analysis of the real samples before and after hydrolysis. Quantitative performance characteristics and the character of the hydrolysis reaction were studied as well. The optimized method was successfully applied to a bacterial culture supernatant real sample containing HSLs. (c) 2007 Elsevier B.V All rights reserved.

Keyword :

hydrolysis hydrolysis N-acylhomoserine lactones N-acylhomoserine lactones N-acylhomoserines N-acylhomoserines optimization optimization quorum sensing quorum sensing UPLC UPLC

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GB/T 7714 Englmann, Matthias , Fekete, Agnes , Kuttler, Christina et al. The hydrolysis of unsubstituted N-acylhomoserine lactones to their homoserine metabolites Analytical approaches using ultra performance liquid chromatography [J]. | JOURNAL OF CHROMATOGRAPHY A , 2007 , 1160 (1-2) : 184-193 .
MLA Englmann, Matthias et al. "The hydrolysis of unsubstituted N-acylhomoserine lactones to their homoserine metabolites Analytical approaches using ultra performance liquid chromatography" . | JOURNAL OF CHROMATOGRAPHY A 1160 . 1-2 (2007) : 184-193 .
APA Englmann, Matthias , Fekete, Agnes , Kuttler, Christina , Frommberger, Moritz , Li, Xiaojing , Gebefuegi, Istvan et al. The hydrolysis of unsubstituted N-acylhomoserine lactones to their homoserine metabolites Analytical approaches using ultra performance liquid chromatography . | JOURNAL OF CHROMATOGRAPHY A , 2007 , 1160 (1-2) , 184-193 .
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Uptake, degradation and chiral discrimination of N-acyl-D/L-homoserine lactones by barley (Hordeum vulgare) and yam bean (Pachyrhizus erosus) plants SCIE
期刊论文 | 2007 , 389 (5) , 1447-1457 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
WoS CC Cited Count: 74
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Bacterial intraspecies and interspecies communication in the rhizosphere is mediated by diffusible signal molecules. Many Gram-negative bacteria use N-acyl-homoserine lactones (AHLs) as autoinducers in the quorum sensing response. While bacterial signalling is well described, the fate of AHLs in contact with plants is much less known. Thus, adsorption, uptake and translocation of N-hexanoyl- (C6-HSL), N-octanoyl- (C8-HSL) and N-decanoyl-homoserine lactone (C10-HSL) were studied in axenic systems with barley (Hordeum vulgare L.) and the legume yam bean (Pachyrhizus erosus (L.) Urban) as model plants using ultra-performance liquid chromatography (UPLC), Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) and tritium-labelled AHLs. Decreases in AHL concentration due to abiotic adsorption or degradation were tolerable under the experimental conditions. The presence of plants enhanced AHL decline in media depending on the compounds' lipophilicity, whereby the legume caused stronger AHL decrease than barley. All tested AHLs were traceable in root extracts of both plants. While all AHLs except C10-HSL were detectable in barley shoots, only C6-HSL was found in shoots of yam bean. Furthermore, tritium-labelled AHLs were used to determine short-term uptake kinetics. Chiral separation by GC-MS revealed that both plants discriminated D-AHL stereoisomers to different extents. These results indicate substantial differences in uptake and degradation of different AHLs in the plants tested.

Keyword :

chiral separation chiral separation FTICR-MS FTICR-MS hordeum vulgare hordeum vulgare pachyrhizus erosus pachyrhizus erosus tritium autoradiography tritium autoradiography UPLC UPLC

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GB/T 7714 Goetz, Christine , Fekete, Agnes , Gebefuegi, Istvan et al. Uptake, degradation and chiral discrimination of N-acyl-D/L-homoserine lactones by barley (Hordeum vulgare) and yam bean (Pachyrhizus erosus) plants [J]. | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2007 , 389 (5) : 1447-1457 .
MLA Goetz, Christine et al. "Uptake, degradation and chiral discrimination of N-acyl-D/L-homoserine lactones by barley (Hordeum vulgare) and yam bean (Pachyrhizus erosus) plants" . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY 389 . 5 (2007) : 1447-1457 .
APA Goetz, Christine , Fekete, Agnes , Gebefuegi, Istvan , Forczek, Sandor T. , Fuksova, Kvetoslava , Li, Xiaojing et al. Uptake, degradation and chiral discrimination of N-acyl-D/L-homoserine lactones by barley (Hordeum vulgare) and yam bean (Pachyrhizus erosus) plants . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2007 , 389 (5) , 1447-1457 .
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