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学者姓名：柯星星
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Abstract ：
Convective Polymerase Chain Reaction (cPCR), owing to its enhanced thermal cycling efficiency, holds promise for application in the next generation of mainstream commercial PCR instruments. Despite its potential, existing capillary-based and annular reaction chamber designs encounter limitations in precisely controlling the internal flow field, which poses a significant barrier to the progression of cPCR. To overcome these obstacles, this work innovatively proposes a cPCR chip utilizing a "racetrack-shaped" reaction chamber, along with a reverse design approach tailored to meet diverse reaction requirements. Through modeling and simulation, we accurately obtained the relationship between the design parameters and the average flow velocity of the cPCR chip with a "racetrack-shaped" reaction chamber. By capturing the motion of fluorescent particles using a high-speed camera, we acquired the velocity distribution of the actual flow field. Further, we utilized these relationships to conduct a reverse design. Ultimately, a reaction chamber was designed based on the actual amplification needs of 2019-nCoV and hepatitis B virus, and successful amplification was achieved using a self-developed temperature control platform.
Keyword ：
convective PCR convective PCR flow field control flow field control microfluidic chip microfluidic chip Rayleigh-B & eacute;nard convection Rayleigh-B & eacute;nard convection reverse design reverse design
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| GB/T 7714 | Li, Chenfei , Xie, Yaping , Yong, Haochen et al. A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields [J]. | CHEMOSENSORS , 2025 , 13 (1) . |
| MLA | Li, Chenfei et al. "A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields" . | CHEMOSENSORS 13 . 1 (2025) . |
| APA | Li, Chenfei , Xie, Yaping , Yong, Haochen , Zhao, Xin , Ke, Xingxing , Wu, Zhigang . A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields . | CHEMOSENSORS , 2025 , 13 (1) . |
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Abstract ：
Distinguished by its exceptional sensitivity and specificity, Polymerase Chain Reaction (PCR) is a pivotal technology for pathogen detection. However, traditional PCR instruments that employ thermoelectric cooling (TEC) are often constrained by cost, efficiency, and performance variability resulting from the fluctuations in ambient temperature. Here, we present a thermal cycler that utilizes electromagnetic induction heating at 50 kHz and anti-freezing water cooling with a velocity of 0.06 m/s to facilitate rapid heating and cooling of the PCR reaction chamber, significantly enhancing heat transfer efficiency. A multi-physics theoretical heat transfer model, developed using the digital twin approach, enables precise temperature control through advanced algorithms. Experimental results reveal average heating and cooling rates of 14.92 degrees C/s and 13.39 degrees C/s, respectively, significantly exceeding those of conventional methods. Compared to commercial PCR instruments, the proposed system further optimizes cost, efficiency, and practicality. Finally, PCR experiments were successfully performed using cDNA (Hepatitis B virus) at various concentrations.
Keyword ：
anti-freezing water cooling anti-freezing water cooling magnetic induction heating magnetic induction heating polymerase chain reaction polymerase chain reaction rapid heat transfer rapid heat transfer thermal cycler thermal cycler
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| GB/T 7714 | Xie, Yaping , Jiang, Qin , Chang, Chang et al. A Thermal Cycler Based on Magnetic Induction Heating and Anti-Freezing Water Cooling for Rapid PCR [J]. | MICROMACHINES , 2024 , 15 (12) . |
| MLA | Xie, Yaping et al. "A Thermal Cycler Based on Magnetic Induction Heating and Anti-Freezing Water Cooling for Rapid PCR" . | MICROMACHINES 15 . 12 (2024) . |
| APA | Xie, Yaping , Jiang, Qin , Chang, Chang , Zhao, Xin , Yong, Haochen , Ke, Xingxing et al. A Thermal Cycler Based on Magnetic Induction Heating and Anti-Freezing Water Cooling for Rapid PCR . | MICROMACHINES , 2024 , 15 (12) . |
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