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学者姓名：罗芳
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Abstract ：
The entry of Hg2+ into the human body can lead to serious damage to some organs and it is vital to construct some simple but sensitive Hg2+ assay methods. A multicolor sensor for Hg2+ was constructed depended on the inhibition of peroxidase activity of Fe/Co-MIL-88(NH2) mediated by DNA and color change caused by the etching of gold nanobipyramids (Au NBPs). The enzymatic activity of Fe/Co-MIL-88(NH2) can be inhibited by adsorbing the T-rich oligonucleotide sequence on its surface. Hg2+ can combine with thymine of the T-rich oligonucleotide sequence to constitute T-Hg2+-T complex, and cause the transforming of T-rich oligonucleotide sequence into three-dimensional structure and desorbed from Fe/Co-MIL-88(NH2) surface. Which results in the restoration of the peroxidase activity of Fe/Co-MIL-88(NH2), and then it catalyzes the 3,3′,5,5′-tetramethylbenzidine (TMB) to engender TMB2+, TMB2+ can etch Au NBPs, this accompanied with a peak shift of the UV–vis spectrum and recognizable color changes of the system. Semi-quantitative can be realized using the multicolor changes of the Au NBPs as the reading mode. Furthermore, quantitative sensing can be achieved by measuring the UV–vis peak shift of the system. The linear response range is 1.0 nM–10 µM, and the detection limit (LOD) is 0.28 nM. © 2023 Elsevier B.V.
Keyword ：
Fe/Co-MIL-88(NH2) Fe/Co-MIL-88(NH2) Gold nanobipyramids Gold nanobipyramids Hg2+ Hg2+ Multicolor Multicolor Nanozyme Nanozyme
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| GB/T 7714 | Luo, Q. , Chen, Y. , Chen, L. et al. Multicolor Hg2+ sensor based on the regulation of peroxidase activity of Fe/Co-MIL-88(NH2) and etching of gold nanobipyramids [J]. | Microchemical Journal , 2023 , 193 . |
| MLA | Luo, Q. et al. "Multicolor Hg2+ sensor based on the regulation of peroxidase activity of Fe/Co-MIL-88(NH2) and etching of gold nanobipyramids" . | Microchemical Journal 193 (2023) . |
| APA | Luo, Q. , Chen, Y. , Chen, L. , Chen, Z. , Luo, F. , Wang, J. et al. Multicolor Hg2+ sensor based on the regulation of peroxidase activity of Fe/Co-MIL-88(NH2) and etching of gold nanobipyramids . | Microchemical Journal , 2023 , 193 . |
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Three-dimensional (3D) ZnO nanostructures were used as the support for gold nanoparticles (AuNPs) and graphite carbon nitride (g-C3N4) to form a Z-scheme heterostructure photoelectrode (3D ZnO/Au/g-C3N4) in this work. The 3D structure of the ZnO nanorods provided a directional channel for fast carrier transport and abundant reaction sites. ZnO and g-C3N4 formed the Z-scheme heterojunction, and the AuNPs sandwiched between ZnO and g-C3N4 acted as the mediators and greatly reinforced vector electron transfer in the Z-scheme heterojunction. Then, the 3D ZnO/Au/g-C3N4 photoelectrode was applied to develop a sensitive photoelectrochemical (PEC) biosensor for reduced glutathione (GSH) detection. GSH can act as a hole scavenger and reduce the recombination of photogenerated electron–hole pairs, which results in an increase in the photocurrent of the system. The enhanced current showed good linearity with GSH concentration in the range of 7–2000 μM, and the system had a limit of detection of 2.3 μM. The proposed biosensor exhibited good selectivity and reproducibility, obtaining satisfactory results in the analysis of the target in human serum. © 2023 The Author(s).
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| GB/T 7714 | Li, W. , Wang, X. , Huang, J. et al. Preparation of Z-scheme 3D ZnO/Au/g-C3N4 heterostructures for the photoelectrochemical sensing of glutathione† [J]. | Sensors and Diagnostics , 2023 , 2 (3) : 699-706 . |
| MLA | Li, W. et al. "Preparation of Z-scheme 3D ZnO/Au/g-C3N4 heterostructures for the photoelectrochemical sensing of glutathione†" . | Sensors and Diagnostics 2 . 3 (2023) : 699-706 . |
| APA | Li, W. , Wang, X. , Huang, J. , Zhao, M. , Yang, J. , Luo, F. et al. Preparation of Z-scheme 3D ZnO/Au/g-C3N4 heterostructures for the photoelectrochemical sensing of glutathione† . | Sensors and Diagnostics , 2023 , 2 (3) , 699-706 . |
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Lead halide perovskites have garnered significant attention in the field of photoelectrochemistry due to their outstanding optical properties. Nevertheless, the pronounced susceptibility of perovskite to water poses a major limitation to its practical applications. A signal-on photoelectrochemical (PEC) sensor was constructed in this work using water-resistant perovskite nanocrystals for the detection of hydrogen sulfide. Specially, the electrode surface was modified with composite nanomaterials (CsPbBr3 @PEG-PCL). In the presence of H2S, efficient heterojunctions were formed through the in-situ generation of PbS on CsPbBr3 @PEG-PCL, thereby enhancing the photocurrent signal. The potential mechanism behind this signal enhancement was extensively discussed. The designed sensor exhibited a linear relationship with the concentration of H2S ranging from 0.02 to 120 mu M, with a detection limit of 6.67 nM. This work opens up new possibilities for utilizing halide perovskites in the field of sensing, with promising applications in areas such as food safety, environmental monitoring, and biomedical analysis.
Keyword ：
H2S H2S Heterojunction Heterojunction Perovskite Perovskite Photoectrochemical sensor Photoectrochemical sensor
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| GB/T 7714 | Zhang, Yating , Zheng, Xiaoyu , Chen, Siqi et al. Signal-on photoelectrochemical sensing by highly stable perovskite nanocrystals based on in situ formation of heterojunction [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 403 . |
| MLA | Zhang, Yating et al. "Signal-on photoelectrochemical sensing by highly stable perovskite nanocrystals based on in situ formation of heterojunction" . | SENSORS AND ACTUATORS B-CHEMICAL 403 (2023) . |
| APA | Zhang, Yating , Zheng, Xiaoyu , Chen, Siqi , Qiu, Yongzhen , Luo, Fang , Weng, Zuquan et al. Signal-on photoelectrochemical sensing by highly stable perovskite nanocrystals based on in situ formation of heterojunction . | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 403 . |
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Presently, numerous state-of-the-art approaches are being adapted for gas sensing and monitoring. These include hazardous gas leak detection as well as ambient air monitoring. Photoionization detectors, electrochemical sensors, and optical infrared sensors are a few of the commonly widely used technologies. Extensive reviews on the current state of gas sensors have been summarized. These sensors, which are either nonselective or semiselective, are affected by unwanted analytes. On the other hand, volatile organic compounds (VOCs) can be heavily mixed in many vapor intrusion situations. To determine the individual VOCs in a highly mixed gas sample using nonselective or semiselective gas sensors, gas separation and discrimination technologies are highly warranted. These technologies include gas permeable membranes, metal-organic frameworks, microfluidics and IR bandpass filters for different sensors, respectively. The majority of these gas separation and discrimination technologies are currently being developed and evaluated in laboratory-controlled environments and have not yet been extensively utilized in the field for vapor intrusion monitoring. These technologies show promise for continued development and application in the field for more complex gas mixtures. Hence, the present review focuses on the perspectives and a summary of the existing gas separation and discrimination technologies for the currently popular reported gas sensors in environmental applications.
Keyword ：
electrochemical gas sensors electrochemical gas sensors environmental gas sensors environmental gas sensors gas separation technologies gas separation technologies optical infrared sensors optical infrared sensors photoionization detectors photoionization detectors volatile organic compounds volatile organic compounds
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| GB/T 7714 | Wang, Liang , Cheng, Ying , Gopalan, Saianand et al. Review and Perspective: Gas Separation and Discrimination Technologies for Current Gas Sensors in Environmental Applications [J]. | ACS SENSORS , 2023 , 8 (4) : 1373-1390 . |
| MLA | Wang, Liang et al. "Review and Perspective: Gas Separation and Discrimination Technologies for Current Gas Sensors in Environmental Applications" . | ACS SENSORS 8 . 4 (2023) : 1373-1390 . |
| APA | Wang, Liang , Cheng, Ying , Gopalan, Saianand , Luo, Fang , Amreen, Khairunnisa , Singh, Ritesh Kumar et al. Review and Perspective: Gas Separation and Discrimination Technologies for Current Gas Sensors in Environmental Applications . | ACS SENSORS , 2023 , 8 (4) , 1373-1390 . |
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T-2 toxin is widely found in nature and also contaminates field crops and grain storage, posing a potential threat to human health. In this study, an ultra-sensitive electrochemiluminescence (ECL) aptasensor has been developed for the detection of T-2 toxin using Ti3C2 MXene-based nanomaterials functionalized with gold nanorods (AuNRs) and Ru(bpy)32+ (Ti3C2@AuNRs-Ru) as the luminescent indicator. Specifically, gold nanorods are grown in situ on the surface of Ti3C2 MXene, and Ru(bpy)32+ is loaded onto Ti3C2@AuNRs via electrostatic force to form the Ti3C2@AuNRs-Ru luminescent probe, which is then modified on the electrode surface. The aptamer of T-2 toxin is connected to the electrode surface through Au-S bonds, and then the capture chain (cDNA) with a quenching probe of ferrocene is introduced to the system. Ferrocene can quench the luminescence of the probe, reducing the ECL signal. In the presence of T-2 toxin, it binds to the aptamer with higher affinity, so the quenching probe is competed down, resulting in the recovery of the ECL signal. The increase in ECL signal is linearly correlated with the logarithm of T-2 toxin concentration, with the detection range of 50 fg/mL to 50 ng/ mL and a detection limit of 6.44 fg/mL. This strategy utilizes the high catalytic activity and excellent conductivity of MXene, improving the sensitivity significantly. The proposed sensor has been successfully applied for the detection of actual samples and provides a new strategy for the detection of hazardous pollutants.
Keyword ：
Electrochemiluminescent aptasensor Electrochemiluminescent aptasensor Hazardous pollutant Hazardous pollutant Nanomaterials Nanomaterials T-2 toxin T-2 toxin
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| GB/T 7714 | Zhang, Junyi , Deng, Ye , Chen, Lifen et al. A novel electrochemiluminescence aptasensor using Ti3C2@AuNRs-Ru for ultra-sensitive detection of T-2 toxin [J]. | ELECTROCHIMICA ACTA , 2023 , 475 . |
| MLA | Zhang, Junyi et al. "A novel electrochemiluminescence aptasensor using Ti3C2@AuNRs-Ru for ultra-sensitive detection of T-2 toxin" . | ELECTROCHIMICA ACTA 475 (2023) . |
| APA | Zhang, Junyi , Deng, Ye , Chen, Lifen , Luo, Fang , Weng, Zuquan , Lin, Cuiying et al. A novel electrochemiluminescence aptasensor using Ti3C2@AuNRs-Ru for ultra-sensitive detection of T-2 toxin . | ELECTROCHIMICA ACTA , 2023 , 475 . |
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The detection of trypsin is significantly important for both clinical diagnosis and disease treatment. In this study, an innovative multicolor sensor for trypsin detection has been established based on the regulation of the peroxidase activity of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) and have slight peroxidase enzyme activity and can catalyze the oxidation of 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) to generate TMB+, while trypsin can hydrolyze BSA ligands on the surface of BSA-Au NCs, thus exposing more catalytic active sites of BSA-Au NCs and resulting in the enhancement of the peroxidase activity of BSA-Au NCs, hence more TMB+ is generated. Under acidic conditions, TMB+ can etch Au NBPs efficiently, consequently affecting the aspect ratio of Au NBPs accompanied by the ultraviolet-visible (UV-vis) spectra blue shifting of the system. Furthermore, this also results in color variations that can be distinguished and recognized by naked eyes without any expensive and sophisticated instruments. This multicolor sensor has an available linear relationship with the logarithm of the trypsin concentration in the range of 0.1-100 mu g/mL, and the detection limit is 0.045 mu g/mL. The designed sensor has been used to detect the concentration of trypsin in human serum samples from healthy individuals and pancreatitis patients with satisfactory results.
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| GB/T 7714 | Luo, Qin , Tian, Mengjian , Luo, Fang et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids [J]. | ANALYTICAL CHEMISTRY , 2023 : 2390-2397 . |
| MLA | Luo, Qin et al. "Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids" . | ANALYTICAL CHEMISTRY (2023) : 2390-2397 . |
| APA | Luo, Qin , Tian, Mengjian , Luo, Fang , Zhao, Min , Lin, Cuiying , Qiu, Bin et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids . | ANALYTICAL CHEMISTRY , 2023 , 2390-2397 . |
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Hyaluronidase (HAase) is a potential tumor biomarker for diseases of the digestive tract and nervous system, the development of simple and sensitive techniques for HAase determination is urgent needed. Gold nanorods (Au NRs) can be etched by H2O2 with high efficiency and display color changing. In this work, a HAase-responsive hydrogel system had been designed and the amount of H2O2 spilled from the system had a close relationship with the amount of HAase, then the spilled H2O2 had been applied to etch Au NRs. The color change of the solution was used to realize semi-quantitative determination of HAase. Furthermore, the longitudinal peak shift of Au NRs had a linear correlation with the concentration of HAase in the range of 10-60 U/mL (within 40 min) and the limit of detection (LOD) was 3.8 U/mL (S/N = 3), which can be used to realize accurate quantitative analysis of HAase. The proposed method has been applied to monitor HAase in serum of pancreatic cancer patients with satisfied results.
Keyword ：
Gold nanorods Gold nanorods Hyaluronidase Hyaluronidase Multicolor biosensor Multicolor biosensor Target response hydrogel Target response hydrogel
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| GB/T 7714 | Wang, Weiquan , Xu, Yunpeng , Hou, Jie et al. Multicolor biosensor for hyaluronidase based on target-responsive hydrogel and etching of gold nanorods by H2O2 [J]. | TALANTA , 2023 , 257 . |
| MLA | Wang, Weiquan et al. "Multicolor biosensor for hyaluronidase based on target-responsive hydrogel and etching of gold nanorods by H2O2" . | TALANTA 257 (2023) . |
| APA | Wang, Weiquan , Xu, Yunpeng , Hou, Jie , Li, Zhixin , Luo, Fang , He, De et al. Multicolor biosensor for hyaluronidase based on target-responsive hydrogel and etching of gold nanorods by H2O2 . | TALANTA , 2023 , 257 . |
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In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the distance between the signal quenching factor ferrocene (Fc) and the ECL signal unit increased, and the repulsion between the signal unit and the Indium Tin Oxides (ITO) electrode decreased. The above results resulted in an enhanced ECL signal. ECL intensity has a good linear relationship with the logarithm of Dam MTase concentration in the range of 5-70 U/ mL with a detection limit of 23.4 mU/mL. The proposed biosensor was successfully utilized to detect of Dam MTase in serum samples.
Keyword ：
Biosensor Biosensor Cas Cas CRISPR CRISPR DNA adenine Methylation methyltransferase DNA adenine Methylation methyltransferase DNA methylation DNA methylation Electrochemiluminescence Electrochemiluminescence
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| GB/T 7714 | Lin, Cuiying , Huang, Qingqing , Tian, Mengjian et al. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement [J]. | TALANTA , 2023 , 251 . |
| MLA | Lin, Cuiying et al. "Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement" . | TALANTA 251 (2023) . |
| APA | Lin, Cuiying , Huang, Qingqing , Tian, Mengjian , Luo, Fang , Wang, Jian , Qiu, Bin et al. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement . | TALANTA , 2023 , 251 . |
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Much progress has been made in the biosensor area with the prosperity and development of nucleic acid nanotechnology and molecular biology. Especially, the advances made in dynamic DNA nanotechnology and the increasing sophistication of toehold-mediated strand displacement reactions have put new energy into nucleic acid-based biosensor designs. The exposure of the gene-editing ability of clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (Cas) has also greatly revolutionized the way nucleic acid can be operated in biosensing systems, providing versatile strategies for signal handling. Hence, in this review, we will discuss the application of these two technologies as the expanded tools for nucleic acid-based biosensors and focus on their strategies governing signal trans-duction. Besides, technologies like DNA computing and deep learning approaches which flashed in nucleic acid biosensors very recently are also discussed in the perspective and outlook part.(c) 2023 Elsevier B.V. All rights reserved.
Keyword ：
Biosensor Biosensor Cas system Cas system CRISPR CRISPR DNA DNA DNA dynamic nanotechnology DNA dynamic nanotechnology Toehold strand displacement Toehold strand displacement
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| GB/T 7714 | Zhang, Fang , Chen, Ganghui , Gao, Chenshan et al. Updated toolkits for nucleic acid-based biosensors [J]. | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2023 , 159 . |
| MLA | Zhang, Fang et al. "Updated toolkits for nucleic acid-based biosensors" . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY 159 (2023) . |
| APA | Zhang, Fang , Chen, Ganghui , Gao, Chenshan , Huang, Shuqin , Mahu, Yishan , Luo, Fang et al. Updated toolkits for nucleic acid-based biosensors . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2023 , 159 . |
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Locating underground pipeline leaks can be challenging due to their hidden nature and variable terrain conditions. To sample soil gas, solid-phase microextraction (SPME) was employed, and a portable gas chromatography/mass spectrometry (GC/MS) was used to detect the presence and concentrations of petroleum hydrocarbon volatile organic compounds (pH-VOCs), including benzene, toluene, ethylbenzene, and xylene (BTEX). We optimized the extraction method through benchtop studies using SPME. The appropriate fibre materials and exposure time were selected for each BTEX compound. Before applying SPME, we preconditioned the soil vapour samples by keeping the temperature at around 4 degrees C and using ethanol as a desorbing agent and moisture filters to minimize the impact of moisture. To conduct this optimisation, airbags were applied to condition the soil vapour samples and SPME sampling. By conditioning the samples using this method, we were able to improve analytical efficiency and accuracy while minimizing environmental impacts, resulting in more reliable research data in the field. The study employed portable GC/MS data to assess the concentration distribution of BTEX in soil vapour samples obtained from 1.5 m below the ground surface at 10 subsurface vapour monitoring locations at the leak site. After optimization, the detection limits of BTEX were almost 100 mu g/m 3 , and the measurement repeatabilities were approximately 5% and 15% for BTEX standards in the laboratory and soil vapour samples in the field, respectively. The soil vapour samples showed a hotspot region with high BTEX concentrations, reaching 30 mg/m 3 , indicating a diesel return pipeline leak caused by a gasket failure in a flange. The prompt detection of the leak source was critical in minimizing environmental impact and worker safety hazards. (c) 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
Keyword ：
mass mass ms) ms) Portable gas chromatography Portable gas chromatography Rapid on -site analysis Rapid on -site analysis Soil vapour BTEX assessment Soil vapour BTEX assessment Solid phase micro -extraction (SPME) Solid phase micro -extraction (SPME) spectrometry (gc spectrometry (gc Underground pipeline leak detection Underground pipeline leak detection
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| GB/T 7714 | Wang, Liang , Cheng, Ying , Wu, Cuiqin et al. Rapid on-site detection of underground petroleum pipeline leaks and risk assessment using portable gas chromatography-mass spectrometry and solid phase microextraction [J]. | JOURNAL OF CHROMATOGRAPHY A , 2023 , 1696 . |
| MLA | Wang, Liang et al. "Rapid on-site detection of underground petroleum pipeline leaks and risk assessment using portable gas chromatography-mass spectrometry and solid phase microextraction" . | JOURNAL OF CHROMATOGRAPHY A 1696 (2023) . |
| APA | Wang, Liang , Cheng, Ying , Wu, Cuiqin , Luo, Fang , Lin, Zhenyu , Naidu, Ravi . Rapid on-site detection of underground petroleum pipeline leaks and risk assessment using portable gas chromatography-mass spectrometry and solid phase microextraction . | JOURNAL OF CHROMATOGRAPHY A , 2023 , 1696 . |
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