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学者姓名：邱彬
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Abstract ：
Combined the electrostatic interaction of the negatively charged gold nanorods (AuNRs) (as acceptor) and Ru(bpy)(3)(2+) (as donor), an electrochemiluminescence resonance energy transfer (ECL-RET) sensor was constructed and applied for the detection of organophosphorus pesticides (OPs). Negatively charged AuNRs were synthesized by modifying AuNRs with polystyrene sulfonate (PSS) firstly, which can bind to Ru(bpy)(3)(2+) through electrostatic interaction so that the luminophore was absorbed by the acceptor, the resonance energy transfer occurred and only low ECL signal had been detected. Thiocholine can be produced by the hydrolysis process of acetylthiocholine (ATCh) with the help of acetylcholinesterase (AChE), which can bond with PSS-modified AuNRs (PSS-AuNRs) through gold-sulfur interaction, this caused the releasing of the adsorbed Ru(bpy)(3)(2+) into the solution and resulting in the restoration of the ECL intensity. However, the activity of AChE was inhibited by OPs, and the recovery process of the ECL signal was thus suppressed as well. In this study, chlorpyrifos was chosen as model target, the results indicated that the correlation between the ECL intensity and the logarithm of chlorpyrifos concentration showed remarkable linearity across 1 ng/mL to 1 mg/mL, achieving a detection limit of 0.51 ng/mL. The proposed system has been utilized for detecting OPs in real samples with satisfied results.
Keyword ：
Acetylcholinesterase Acetylcholinesterase Electrochemiluminescence Electrochemiluminescence Gold nanorods Gold nanorods Organophosphorus pesticides Organophosphorus pesticides Resonance energy transfer Resonance energy transfer
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| GB/T 7714 | Li, Zixin , Lin, Zeyu , Chen, Lifen et al. Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+ [J]. | TALANTA , 2025 , 281 . |
| MLA | Li, Zixin et al. "Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+" . | TALANTA 281 (2025) . |
| APA | Li, Zixin , Lin, Zeyu , Chen, Lifen , Lin, Yue , Luo, Fang , Lin, Cuiying et al. Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+ . | TALANTA , 2025 , 281 . |
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Metal-organic frameworks, which are the desired candidates for biosensing application due to their tunable properties, are significantly hindered by their rapid degradation in aqueous solutions, as well as the loss of their inherent fluorescence. Most studies aim to improve the hydrophobicity of materials by modifying their contact angle, thereby enhancing water stability. However, water droplets poorly adhere to the surface of hydrophobic materials, limiting their application for direct contact and detection in aqueous environments. Drawing inspiration from the sacrificial protection mechanism of butterfly wings used to evade predation and entanglement, a universal approach is successfully developed to protect water-sensitive MIL-MOFs from water molecule attack while preserving good hydrophilicity. Using the organic ligand 2,2 '-bipyridine-5,5 '-dicarboxylic acid (bpydc) as sacrificial protection scales, the MIL-125-NH2-bpydc demonstrated broad pH structural stability (pH 2-12) and fluorescence stability increased by 10.17 time in aqueous solutions, achieving the highest performance in MILMOFs. The MIL-125-NH2-bpydc is biocompatible enabling it to perform long-term fluorescence imaging in living cells and zebrafish, further detecting hydrogen sulfide (H2S) in the aqueous and biological systems via turn-on fluorescence emission. This study offers a novel and universal sacrifice-protection strategy for the design and development of the luminescent biocompatible MOFs tailored for biosensing applications.
Keyword ：
fluorescence fluorescence H2S H2S metal-organic frameworks metal-organic frameworks sacrifice-protection strategy sacrifice-protection strategy water stable water stable
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| GB/T 7714 | Weng, Zhanglin , Xie, Zhijie , Wu, Xiaoping et al. Water-Stable MIL-MOFs Developed Through a Novel Sacrifice-Protection Strategy Inspired by Butterfly Wings' Scales for Long-Term Turn-On Fluorescence Sensing of H2S [J]. | SMALL METHODS , 2025 . |
| MLA | Weng, Zhanglin et al. "Water-Stable MIL-MOFs Developed Through a Novel Sacrifice-Protection Strategy Inspired by Butterfly Wings' Scales for Long-Term Turn-On Fluorescence Sensing of H2S" . | SMALL METHODS (2025) . |
| APA | Weng, Zhanglin , Xie, Zhijie , Wu, Xiaoping , Qiu, Bin , Chen, Juanjuan , Sun, Weiming et al. Water-Stable MIL-MOFs Developed Through a Novel Sacrifice-Protection Strategy Inspired by Butterfly Wings' Scales for Long-Term Turn-On Fluorescence Sensing of H2S . | SMALL METHODS , 2025 . |
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Real time detection of hydrogen sulfide (H2S) in deep brain regions is of great significance. Photoelectrochemical (PEC) biosensors hold promise for real-time monitoring but low sensitivity due to the use of small electrode restrained its application. In this study, a miniaturized sensor that integrates organic photoelectrochemical transistors (OPECTs) with optical fiber (OF) microelectrodes was designed for the first time, which can amplify the small PEC signal into large channel current change. A conductive gold layer and cuprous oxide nanoparticles (Cu2O NPs) were sequentially deposited onto the OF, followed by a Nafion polymer coating to enhance anti-interference capabilities. Cu2O reacts with H2S to generate Cu2S. The resulting Cu2O/Cu2S heterojunction induces changes in the PEC signal and channel current. Compared to a conventional PEC system, the OPECT sensor has higher sensitivity and lower detection limit, enabling monitoring of H2S concentration fluctuations within the rat brain, which demonstrates the approach's capability for highly sensitive in vivo biomarker detection.
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| GB/T 7714 | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (13) : 7467-7475 . |
| MLA | Zeng, Yulan et al. "Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection" . | ANALYTICAL CHEMISTRY 97 . 13 (2025) : 7467-7475 . |
| APA | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui , Luo, Fang , Qiu, Bin , Fu, Fengfu et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection . | ANALYTICAL CHEMISTRY , 2025 , 97 (13) , 7467-7475 . |
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A highly efficient and sensitive non-enzymatic glucose sensor fabricated using NiO/NiPc composite nanomaterials was introduced by this study. The sensor employed a unique design by modifying the ITO electrode with the NiO/NiPc heterojunction. This enhancement was achieved by improving charge separation and transfer through the type-II heterojunction formed between NiO and NiPc. The integration of NiO/NiPc composite materials also improved the electrocatalytic activity. This enhancement was attributed to the increased concentration of oxygen vacancies, thereby boosting the overall performance of the sensor. The results of the sensor application demonstrated that under the optimized conditions (pH 4.0, -0.3 V bias), the sensor exhibited remarkable sensitivity and selectivity towards glucose, with a linear relationship between the photocurrent change and glucose concentration. The detection limit was as low as 0.016 nM, and the sensitivity was 4.146 mu A & sdot;mM- 1 & sdot;cm- 2. The sensor demonstrated excellent repeatability, stability, and high recovery rate in real sweat sample testing, highlighting its great potential for practical glucose detection applications.
Keyword ：
Glucose Glucose NiO NiO NiPc NiPc Photoelectrochemical sensor Photoelectrochemical sensor
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| GB/T 7714 | Xue, Xiaoyan , Wu, Wenru , Yang, Yating et al. Oxygen vacancy-enriched NiO/NiPc heterojunction for highly efficient and sensitive non-enzymatic glucose sensing [J]. | MICROCHEMICAL JOURNAL , 2025 , 208 . |
| MLA | Xue, Xiaoyan et al. "Oxygen vacancy-enriched NiO/NiPc heterojunction for highly efficient and sensitive non-enzymatic glucose sensing" . | MICROCHEMICAL JOURNAL 208 (2025) . |
| APA | Xue, Xiaoyan , Wu, Wenru , Yang, Yating , Lin, Yue , Luo, Fang , Lin, Cuiying et al. Oxygen vacancy-enriched NiO/NiPc heterojunction for highly efficient and sensitive non-enzymatic glucose sensing . | MICROCHEMICAL JOURNAL , 2025 , 208 . |
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The persistence of T-2 toxin in food and feed matrices renders it a pervasive contaminant, impacting both human and animal health. Traditional detection methods suffer from cumbersome instrumentation and intricate procedures, rendering on-site detection of T-2 toxin unfeasible. Therefore, we have constructed a real-time detection method for T-2 toxin detection by employing a target-responsive DNA hydrogel in conjunction with potassium iodide starch test paper. This method integrates both colorimetric and distance-based signal outputs, offering a streamlined and effective approach for the on-site detection of T-2 toxin. The specific binding of the target to the aptamer in the DNA hydrogel results in the collapse of the hydrogels structure, which changes the viscosity of the system and released horseradish peroxidase in the hydrogel wrapped, and then produces blue-purple marks of different lengths on the starch iodide papers to achieve the specific detection of T-2 toxin. Under optimized conditions, the assay exhibits a detection range spanning from 10 ng/mL to 10 mg/mL for the toxin, with a detection limit of 12.83 ng/mL. The proposed method has been successfully applied for the detection of real corn samples with satisfied result. Such colorimetric-distance dual signal detection method offers notable advantages, including straightforward operation, clear signal interpretation, and practical utility. Its implementation enables rapid, on-the-spot detection of T-2 toxin, particularly beneficial in resource-limited regions and less developed countries.
Keyword ：
Point-of-care testing Point-of-care testing Starch iodide paper Starch iodide paper T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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| GB/T 7714 | Deng, Ye , Lin, Jiarong , Wang, Jingxuan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper [J]. | MICROCHEMICAL JOURNAL , 2025 , 210 . |
| MLA | Deng, Ye et al. "Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper" . | MICROCHEMICAL JOURNAL 210 (2025) . |
| APA | Deng, Ye , Lin, Jiarong , Wang, Jingxuan , Lin, Yue , Luo, Fang , Weng, Zuquan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper . | MICROCHEMICAL JOURNAL , 2025 , 210 . |
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Hirschsprung's disease (HSCR), a congenital condition characterized by the absence of nerve cells in the intestinal wall, often requires early and accurate diagnosis for optimal patient outcomes. In this study, we developed a novel and ultrasensitive biosensing strategy for the detection of HSCR-related microRNAs (miRNAs) by integrating catalytic hairpin assembly (CHA) with CRISPR-Cas12a technology. A two-stage process consists of array recognition, and a universal readout is designed. In the first stage, target miRNAs are recognized and amplified on a solid-phase microarray, while in the second stage, the accumulated conversion chains which are not related to target sequences, activate Cas12a, leading to the cleavage of reporter DNA and the generation of a fluorescence signal spatially separated from the first stage. The proposed method was validated for the comprehensive detection of HSCR-related miRNAs and demonstrated high sensitivity and specificity. This work represents a significant advancement in miRNA diagnostics and holds potential for broader clinical applications.
Keyword ：
Cas12a Cas12a Catalytic hairpin assembly Catalytic hairpin assembly Fluorescence spectrometry Fluorescence spectrometry Hirschsprung's disease Hirschsprung's disease MicroRNAs MicroRNAs
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| GB/T 7714 | Liu, Mingkun , Yan, Lei , Lin, Zhixiong et al. CHA-based microarray with Cas12a universal readout for multiple microRNA detection [J]. | MICROCHIMICA ACTA , 2025 , 192 (5) . |
| MLA | Liu, Mingkun et al. "CHA-based microarray with Cas12a universal readout for multiple microRNA detection" . | MICROCHIMICA ACTA 192 . 5 (2025) . |
| APA | Liu, Mingkun , Yan, Lei , Lin, Zhixiong , Wu, Dianming , Qiu, Bin , Weng, Shangeng . CHA-based microarray with Cas12a universal readout for multiple microRNA detection . | MICROCHIMICA ACTA , 2025 , 192 (5) . |
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In this study, we present the innovative design and comprehensive evaluation of a novel point-of-care testing (POCT) methodology for the rapid and accurate detection of T-2 toxin, a potent mycotoxin with significant implications for food safety and human health. The cornerstone of this approach lies in the integration of a copper-based conductive metal-organic framework (Cu3(HHTP)2) with a target-responsive DNA hydrogel system, creating a dual-signal readout mechanism that significantly enhances detection sensitivity and specificity. Specifically, the hydrogel covers the material's surface metal active sites, which become exposed upon target addition due to hydrogel collapse. The released Cu3(HHTP)2 can catalyze the oxidation of 3,3 ',5,5 '-tetrame-thylbenzidine, resulting in a colorimetric and temperature change in the solution. This dual-mode detection strategy enables both qualitative assessment through direct visual inspection of color change and quantitative analysis by monitoring the solution temperature variation post laser irradiation with a thermometer. Under the optimized conditions, the detection system demonstrates a wide range spanning from 5 to 200 ng/mL, with a detection limit of 1.67 ng/mL. This method has been successfully demonstrated through the analysis of real-world samples, yielding encouraging results that underscore its reliability and effectiveness. The proposed dual-signal approach boasts advantages such as straightforward operational procedures, unambiguous signal outputs, and robust practicality, making it an attractive option for mycotoxin detection in resource-constrained settings and developing regions.
Keyword ：
Cu3(HHTP)2 Cu3(HHTP)2 Point-of-care testing Point-of-care testing T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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| GB/T 7714 | Lin, Jiarong , Deng, Ye , Lin, Yue et al. Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 438 . |
| MLA | Lin, Jiarong et al. "Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel" . | SENSORS AND ACTUATORS B-CHEMICAL 438 (2025) . |
| APA | Lin, Jiarong , Deng, Ye , Lin, Yue , Luo, Fang , Weng, Zuquan , Wang, Jian et al. Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel . | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 438 . |
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Our preliminary investigation has identified the potential of serum fucosylated extracellular vesicles (EVs) miR-4732-5p in the early diagnosis of lung adenocarcinoma (LUAD) by a fucose-captured strategy utilizing lentil lectin (LCA)-magnetic beads and subsequent screening of high throughput sequencing and validation of real-time quantitative polymerase chain reaction (RT-qPCR). Considering the relatively complicated procedure, expensive equipment, and stringent laboratory condition, we have constructed an electrochemical biosensor assay for the detection of miR-4732-5p. miR-4732-5p is extremely low in serum, down to the fM level, so it needs to be detected by highly sensitive electrochemical methods based on the Mg2+-dependent DNAzyme splitting nucleic acid lock (NAL) cycle and hybridization chain reaction (HCR) signal amplification. In this study, signal amplification is achieved through the dual amplification reactions using NAL cycle in combination with HCR. In addition, hybridized DNA strands bind to a large number of methylene blue (MB) molecules to enhance signaling. Based on the above strategy, we further enhance our signal amplification strategies to improve detection sensitivity and accuracy. The implementation of this assay proceeded as follows: initially, miR-4732-5p was combined with NAL, and then Mg2+-dependent DNAzyme splitted NAL to release auxiliary DNA (S1) strands, which were subsequently captured by the immobilized capture probe DNA (C1) strands on the electrode surface. Following this, abundant quantities of DNA1 (H1) and DNA2 (H2) tandems were generated by HCR, and S1 strands then hybridized with the H1 and H2 tandems through base complementary pairing. Finally, MB was bonded to the H1 and H2 tandems through pi-pi stacking interaction, leading to the generation of a signal current upon the detection of a potential capable of inducing a redox change of MB by the electrode. Furthermore, we evaluated the performance of our developed electrochemical biosensor assay. The results demonstrated that our assay is a reliable approach, characterized by its high sensitivity (with a detection limit of 2.6x10(-17) M), excellent specificity, good accuracy, reproducibility, and stability. Additionally, it is cost-effective, requires simple operation, and is portable, making it suitable for the detection of serum fucosylated extracellular vesicles miR-4732-5p. Ultimately, this development has the potential to enhance the diagnostic efficiency for patients with early-stage LUAD.
Keyword ：
Biomarker Biomarker Diagnostic Diagnostic Electrochemical biosensor Electrochemical biosensor Lung adenocarcinoma Lung adenocarcinoma miR-4732-5p miR-4732-5p
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| GB/T 7714 | Zhu, Shengting , Chen, Jianlin , Yu, Lili et al. Detection of fucosylated extracellular vesicles miR-4732-5p related to diagnosis of early lung adenocarcinoma by the electrochemical biosensor [J]. | SCIENTIFIC REPORTS , 2024 , 14 (1) . |
| MLA | Zhu, Shengting et al. "Detection of fucosylated extracellular vesicles miR-4732-5p related to diagnosis of early lung adenocarcinoma by the electrochemical biosensor" . | SCIENTIFIC REPORTS 14 . 1 (2024) . |
| APA | Zhu, Shengting , Chen, Jianlin , Yu, Lili , Li, Jiawen , You, Shumin , Zheng, Yue et al. Detection of fucosylated extracellular vesicles miR-4732-5p related to diagnosis of early lung adenocarcinoma by the electrochemical biosensor . | SCIENTIFIC REPORTS , 2024 , 14 (1) . |
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Changes in the charge density on the inner surface of the microchannel can modulate the ion concentration at the tip, thus causing changes in the resistance of the system. In this study, this property is adopted to construct a portable sensor using a multimeter and aflatoxin B1 (AFB1) is used as the model target. Initially, the cDNA/aptamer complex is modified in the microchannel. The inner microchannel surface's charge density is then altered by the recognition of the target, leading to a change in the system's resistance, which can be conveniently monitored using a multimeter. Critical parameters influencing the performance of the system are optimized. Under optimum conditions, the resistance is linearly related to the logarithm of AFB1 concentration in the range of 100 fM-10 nM and the detection limit is 46 fM (S/N = 3). The resistive measurement is separated from the recognition reaction of the target, reducing the matrix interference during the detection process. This sensor boasts high sensitivity and specificity coupled with commendable reproducibility and stability. It is applied to assay the AFB1 content successfully in an actual sample of corn. Moreover, this approach is cost-effective, user-friendly, and highly accurate.
Keyword ：
aflatoxin B1 aflatoxin B1 microchannel microchannel portablesensor portablesensor resistance resistance surface charge density surface charge density
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| GB/T 7714 | Cai, Huabin , Huang, Yanling , Lin, Yue et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout [J]. | ACS SENSORS , 2024 , 9 (1) : 494-501 . |
| MLA | Cai, Huabin et al. "Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout" . | ACS SENSORS 9 . 1 (2024) : 494-501 . |
| APA | Cai, Huabin , Huang, Yanling , Lin, Yue , Luo, Fang , Chen, Lifen , Guo, Longhua et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout . | ACS SENSORS , 2024 , 9 (1) , 494-501 . |
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The control of nanoparticle morphology can effectively change their properties. Adjusting the aggregation state of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) can regulate the enzyme-mimicking catalytic activity. Hyaluronic acid (HA) induces the aggregation of BSA-Au NCs, leading to the shielding of the catalytic active sites and a decrease in activity; this feature has been utilized to design a multicolor biosensor for hyaluronidase (HAase). The presence of HAase can hydrolyze the glycosidic bonds in HA, causing the aggregated BSA-Au NCs to disperse and express their catalytic activity, which in turn catalyzes the etching of Au nanobipyramids (Au-NBPs) in the presence of 3,3 ',5,5 ' -tetramethylbenzidine (TMB), resulting in a morphological transition from bipyramidal to ellipsoidal and spherical shapes, while the solution displays a variety of colors. Visual recognition of these multicolor changes establishes a relationship between the enzyme activity and color. Based on the controlled aggregation and dispersion of BSA-Au NCs and the etching of Au-NBPs, a simple multicolor HAase biosensor was designed. The proposed biosensor shows a linear response to HAase concentrations in the range of 5-80 U/mL, with clear color changes under optimized conditions. The limit of detection (LOD) of the sensor was determined to be 1.98 U/mL (LOD = 3s/k). This method successfully applies the changing of enzyme-mimicking catalytic activity nanoparticles for colorimetric analysis detection.
Keyword ：
aggregation statemodulation aggregation statemodulation Au nanocluster Au nanocluster hyaluronidase hyaluronidase multicolor multicolor nanozymes nanozymes
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| GB/T 7714 | Tian, Mengjian , Lin, Cuiying , Lin, Yue et al. Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State [J]. | ACS APPLIED NANO MATERIALS , 2024 , 7 (5) : 5620-5627 . |
| MLA | Tian, Mengjian et al. "Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State" . | ACS APPLIED NANO MATERIALS 7 . 5 (2024) : 5620-5627 . |
| APA | Tian, Mengjian , Lin, Cuiying , Lin, Yue , Luo, Fang , Qiu, Bin , Wang, Jian et al. Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State . | ACS APPLIED NANO MATERIALS , 2024 , 7 (5) , 5620-5627 . |
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