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Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor SCIE
期刊论文 | 2025 , 27 | WATER RESEARCH X
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Abstract :

This study attempted to compare the enrichment of complete ammonium oxidation (comammox) bacteria, which are affiliated with Nitrospira and not able to generate nitrous oxide (N2O, a potent greenhouse gas) through biological pathways, in two commonly-utilized configurations of floccular sludge reactors, i.e., sequencing batch reactor (SBR) and continuous stirred tank reactor (CSTR), under the ammonium condition of mainstream wastewater (i.e., 40.0 g-N/m(3)). The results in terms of nitrification performance and microbial analyses during 216-d operation showed that compared with SBR offering a fluctuating but generally higher in-situ ammonium concentration (i.e., 1.0-6.0 g-N/m(3)) which was favorable for the growth of ammonium-oxidizing bacteria (AOB, belonging to Nitrosomonas in this study), CSTR managed to lower the in-situ ammonium level to < 2.0 g-N/m(3), thus creating a competitive advantage for comammox bacteria with a highly oligotrophic lifestyle. Such an argument was further supported by dedicated batch tests which revealed that Nitrospira-dominant sludge had a lower maximum ammonium oxidation rate and lower apparent ammonium and oxygen affinity constants than Nitrosomonas-dominant sludge (i.e., 33.5 +/- 2.1 mg-N/h/g-MLVSS vs. 139.9 +/- 26.7 mg-N/h/g-MLVSS, 1.1 +/- 0.1 g-N/m(3) vs. 17.6 +/- 4.6 g-N/m(3), and 0.017 +/- 0.002 g-O-2/m(3) vs. 0.037 +/- 0.013 g-O-2/m(3), respectively), proving the nature of comammox bacteria as a K-strategist. Overall, this study not only provided useful insights into the effective enrichment of comammox bacteria in floccular sludge but also further revealed the interactions between comammox bacteria and AOB, thereby contributing to the future development of comammox-inclusive biological nitrogen removal technologies for sustainable wastewater treatment.

Keyword :

Ammonium-oxidizing bacteria (AOB) Ammonium-oxidizing bacteria (AOB) Complete ammonium oxidation (comammox) bacteria Complete ammonium oxidation (comammox) bacteria Continuous stirred tank reactor (CSTR) Continuous stirred tank reactor (CSTR) r/K-strategists r/K-strategists Sequencing batch reactor (SBR) Sequencing batch reactor (SBR)

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GB/T 7714 Zhu, Ying , Hou, Jiaying , Meng, Fangang et al. Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor [J]. | WATER RESEARCH X , 2025 , 27 .
MLA Zhu, Ying et al. "Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor" . | WATER RESEARCH X 27 (2025) .
APA Zhu, Ying , Hou, Jiaying , Meng, Fangang , Xu, Meiying , Lin, Limin , Yang, Linyan et al. Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor . | WATER RESEARCH X , 2025 , 27 .
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New insights to chlorination-induced chemical bond cleavage in polyamide membranes: Degradation mechanisms and the role of calcium and magnesium ions SCIE
期刊论文 | 2025 , 505 | CHEMICAL ENGINEERING JOURNAL
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Abstract :

This study aims to formulate the degradation mechanism of polyamide membrane by chlorine, and to assess the role of Ca2+ or Mg2+ involved in chlorination. By adjusting chlorination pH, two competing degradation mechanisms, namely chlorination-promoted hydrogen bond cleavage and chlorination-promoted hydrolysis, were first time proposed. Hydrogen bond cleavage promoted severe compaction (reduced pore radius), while hydrolysis led to a loose but non-compactable structure (increased pore radius), causing opposite trends in membrane filtration performance at different pHs. The pore radius and water flux were reduced by 33% and 69% at chlorination pH 4.0, however, water flux was increased by 45% at chlorination pH 10.0. Therefore, intermolecular rather than intramolecular bonds regulate the rotational freedom and then affect compactness of polyamide layers under pressure. Ca2+ or Mg2+ further amplified these effects of chlorine, i.e., water flux was further reduced by 7%-10% at pH 4.0 and further increased by 23%-48% at pH 7.0-10.0. The coordination between carbonyl oxygen and Ca2+ or Mg2+, evidenced by simulated molecular electrostatic potential and binding energies, initiated excessive hydrogen bond breakage between C--O and N-H. Consequently, it prompted N-chlorination, as non-hydrogen-bonded N-H has a higher chlorination priority than hydrogen- bonded N-H. In addition, Ca2+ or Mg2+ accelerated chlorination-promoted hydrolysis.

Keyword :

Calcium Calcium Chlorination Chlorination Hydrogen bond Hydrogen bond Hydrolysis Hydrolysis Magnesium Magnesium Polyamide membranes Polyamide membranes

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GB/T 7714 Yu, Qinyu , Wu, Shuang , Yang, Linyan et al. New insights to chlorination-induced chemical bond cleavage in polyamide membranes: Degradation mechanisms and the role of calcium and magnesium ions [J]. | CHEMICAL ENGINEERING JOURNAL , 2025 , 505 .
MLA Yu, Qinyu et al. "New insights to chlorination-induced chemical bond cleavage in polyamide membranes: Degradation mechanisms and the role of calcium and magnesium ions" . | CHEMICAL ENGINEERING JOURNAL 505 (2025) .
APA Yu, Qinyu , Wu, Shuang , Yang, Linyan , Chen, Xueming , Tao, Min , Wu, Yi et al. New insights to chlorination-induced chemical bond cleavage in polyamide membranes: Degradation mechanisms and the role of calcium and magnesium ions . | CHEMICAL ENGINEERING JOURNAL , 2025 , 505 .
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Degradation of polyamide nanofiltration membranes by free chlorine and halide ions: Kinetics, mechanisms, and implications SCIE
期刊论文 | 2025 , 272 | WATER RESEARCH
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The kinetics of polyamide membrane degradation by free chlorine and halide ions (Br- and Cl-) were innovatively evaluated based on physicochemical properties and filtration performance, using water/solute permeability coefficient in addition to bromide incorporation as important indicators. The reaction rate constants for the reduced water and H3BO3 permeability coefficient were 1-2 orders of magnitude higher at 0-1 h than 1-10 h. N-bromination and bromination-promoted hydrolysis are dominant degradation mechanisms at 0-1 h (reflected by the breakage of hydrogen bond, the increased Ca binding content, and the increased charge density), and ring-bromination further occurs at 1-10 h (reflected by the disappearance or weakening of aromatic amide band and the nearly constant hydrogen bond). The more reactive but less abundant brominating agents (Br2O, BrOCl, BrCl, and Br-2) played significant roles in membrane degradation, contradicting the conventional belief that HOBr is the only reactive species. BrCl at pH 4.0 and BrOCl and Br2O at pH 7.0 made significantly higher contributions to membrane degradation than HOBr (>76 % vs. <13 %). The increased contribution of BrCl and Br-2 with the increased [Cl-] and [Br-](ex) (the excess bromide, defined as [Br-](o) - [HOCl](o) when [Br-](o) > [HOCl](o)), respectively, was responsible for the greater reduction of water permeability coefficient. The innovative and simple approach developed in this study provides important insights to evaluate and predict membrane degradation.

Keyword :

Halogenating agents Halogenating agents Halogenation Halogenation Membrane degradation Membrane degradation Polyamide membranes Polyamide membranes Reaction kinetics Reaction kinetics

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GB/T 7714 Yang, Linyan , Yu, Haixiang , Zhao, Huihui et al. Degradation of polyamide nanofiltration membranes by free chlorine and halide ions: Kinetics, mechanisms, and implications [J]. | WATER RESEARCH , 2025 , 272 .
MLA Yang, Linyan et al. "Degradation of polyamide nanofiltration membranes by free chlorine and halide ions: Kinetics, mechanisms, and implications" . | WATER RESEARCH 272 (2025) .
APA Yang, Linyan , Yu, Haixiang , Zhao, Huihui , Xia, Caiping , Yu, Qinyu , Chen, Xueming et al. Degradation of polyamide nanofiltration membranes by free chlorine and halide ions: Kinetics, mechanisms, and implications . | WATER RESEARCH , 2025 , 272 .
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Unlocking Drivers of Country-Specific Sensitivities of Atmospheric Greenhouse Gas Accumulation: Preparing for Future Pandemic Management SCIE
期刊论文 | 2025 , 59 (1) , 362-372 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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Abstract :

The global atmospheric greenhouse gas (GHG) accumulation trajectory has been subjected to fluctuations in the context of the COVID-19 pandemic. Country-level virus prevalence and geography conditions added complexity to understanding atmospheric GHG accumulation sensitivities in terms of the growth rate. Here, extensive data sets were comprehensively analyzed to capture historical and projected fate of atmospheric GHG concentrations. Although a temporary slowdown was observed during the lockdown, global atmospheric GHG growing rates exhibited a sharp rebound during the early economic recovery after COVID-19, which would threaten climate goals without proactive measures. Despite this consistent global trend, various countries demonstrated differential relative changes in growth rates, representing their specific responses to the pandemic crisis. After systematic consideration of socio-economic and demographic factors and employment of optimal regression models, transportation and industry variables emerged as the strongest predictors for country-specific GHG accumulation sensitivities during lockdown and recovery phases, respectively. Addressing global health and climate change issues would necessitate sustainable government actions and economic decisions in anticipation of future pandemic-related events.

Keyword :

atmospheric greenhouse gas concentration atmospheric greenhouse gas concentration driving forces driving forces growth rate growth rate machine learning machine learning pandemic-related periods pandemic-related periods

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GB/T 7714 Wang, Chen , Wei, Wei , Chen, Xueming et al. Unlocking Drivers of Country-Specific Sensitivities of Atmospheric Greenhouse Gas Accumulation: Preparing for Future Pandemic Management [J]. | ENVIRONMENTAL SCIENCE & TECHNOLOGY , 2025 , 59 (1) : 362-372 .
MLA Wang, Chen et al. "Unlocking Drivers of Country-Specific Sensitivities of Atmospheric Greenhouse Gas Accumulation: Preparing for Future Pandemic Management" . | ENVIRONMENTAL SCIENCE & TECHNOLOGY 59 . 1 (2025) : 362-372 .
APA Wang, Chen , Wei, Wei , Chen, Xueming , Liu, Yiwen , Wijayaratna, Kasun , Ni, Bing-Jie . Unlocking Drivers of Country-Specific Sensitivities of Atmospheric Greenhouse Gas Accumulation: Preparing for Future Pandemic Management . | ENVIRONMENTAL SCIENCE & TECHNOLOGY , 2025 , 59 (1) , 362-372 .
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Microbially-driven alkaline regulation: Organic acid secretion behavior of Penicillium oxalicum and charge neutralization in bauxite residue SCIE
期刊论文 | 2024 , 240 | ENVIRONMENTAL RESEARCH
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Microbially-driven alkaline neutralization in bauxite residue by functional microorganisms is a promising approach for the ecological rehabilitation on alkaline disposal areas. However, the alkali resistance and acid secretion mechanism of functional microorganisms are still unknown, which limits their application. Here, saline-alkaline resistance, acid production performance, and differentially expressed genes of Penicillium oxalicum (P. oxalicum, a functional fungus screened from a typical disposal area) were investigated and its bioneutralization efficiency was evaluated. This fungus exhibited high tolerance to alkalinity (pH 12), and salinity (NaCl 2.0 M), and produced a large amount of oxalic acid to reduce the medium pH to 2.0. Transcriptome showed that alkali stress induced the overexpression of genes related to antioxidant and stress-resistant enzymes (GST, KatE) and glycolytic pathway rate-limiting enzymes (HK). The rate of glycolysis and other organic acid metabolism processes was increased with higher stress resistance of P. oxalicum. The integrated application of P. oxalicum and maize straw accelerated the dissolved organic carbon content and stabilized the leachate pH of bauxite residue at about 7.4. 3DEEM and BIOSEM analysis indicated that P. oxalicum maintained high activity in the residue leachate and continuously decomposed the maize straw for their metabolism. P. oxalicum showed strong alkaline resistance, biomass degradation capacity, and alkaline regulation potential, which should be beneficial for microbial-driven alkaline regulation in bauxite residue.

Keyword :

Alkaline regulation Alkaline regulation Bauxite residue Bauxite residue Ecological rehabilitation Ecological rehabilitation Organic acid secretion Organic acid secretion Penicillium oxalicum Penicillium oxalicum

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GB/T 7714 Zhang, Yifan , Gao, Hui , Zhao, Dongliang et al. Microbially-driven alkaline regulation: Organic acid secretion behavior of Penicillium oxalicum and charge neutralization in bauxite residue [J]. | ENVIRONMENTAL RESEARCH , 2024 , 240 .
MLA Zhang, Yifan et al. "Microbially-driven alkaline regulation: Organic acid secretion behavior of Penicillium oxalicum and charge neutralization in bauxite residue" . | ENVIRONMENTAL RESEARCH 240 (2024) .
APA Zhang, Yifan , Gao, Hui , Zhao, Dongliang , Chen, Xueming , Zhu, Feng , Li, Yinsheng et al. Microbially-driven alkaline regulation: Organic acid secretion behavior of Penicillium oxalicum and charge neutralization in bauxite residue . | ENVIRONMENTAL RESEARCH , 2024 , 240 .
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Bioaugmentation of microalgae fermentation with yeast for enhancing microbial chain elongation: In-situ ethanol production and metabolic potential SCIE
期刊论文 | 2024 , 498 | CHEMICAL ENGINEERING JOURNAL
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Anaerobic microalgae fermentation, leveraging its cost-effectiveness and the adaptability of mixed cultures, holds promise for carboxylate biosynthesis. Microalgae, with their abundant carbohydrates and proteins, stand out as an optimal substrate for this process among various options. Furthermore, microalgae fermentation not only shows the potential to mitigate risks associated with algae blooms but also aligns with the need for sustainable practices. However, the limited utilization rate of microalgae in anaerobic fermentation poses challenges to achieving high production rates of desired products. In this study, we implemented a bioaugmentation process with yeast to enhance carboxylate production performance of microalgae fermentation. The results demonstrated a fourfold increase in carboxylate yield with the addition of yeast. In-situ ethanol production facilitated the conversion of short-chain carboxylic acids into medium-chain carboxylates, achieving a yield of 46.3 mM-C/g VS. The presence of yeast significantly enhanced substrate utilization from 20 % to 80 %, steering the metabolic pathway towards chain elongation. Metagenomic analysis further revealed metabolic shifts following yeast addition, particularly an increased abundance of genes involved in acetyl-CoA production. Notably, the aldehyde:ferredoxin oxidoreductase (AOR) pathway emerged as a key driver in butanol production. These findings highlight the improved performance of anaerobic microalgae fermentation with yeast, enabling efficient production of higher value bioproducts while eliminating the need for external electron donors.

Keyword :

Anaerobic fermentation Anaerobic fermentation Butanol Butanol Medium-chain carboxylic acids Medium-chain carboxylic acids Microalgae Microalgae Yeast bioaugmentation Yeast bioaugmentation

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GB/T 7714 Shi, Xingdong , Ju, Feng , Wei, Wei et al. Bioaugmentation of microalgae fermentation with yeast for enhancing microbial chain elongation: In-situ ethanol production and metabolic potential [J]. | CHEMICAL ENGINEERING JOURNAL , 2024 , 498 .
MLA Shi, Xingdong et al. "Bioaugmentation of microalgae fermentation with yeast for enhancing microbial chain elongation: In-situ ethanol production and metabolic potential" . | CHEMICAL ENGINEERING JOURNAL 498 (2024) .
APA Shi, Xingdong , Ju, Feng , Wei, Wei , Wu, Lan , Chen, Xueming , Ni, Bing-Jie . Bioaugmentation of microalgae fermentation with yeast for enhancing microbial chain elongation: In-situ ethanol production and metabolic potential . | CHEMICAL ENGINEERING JOURNAL , 2024 , 498 .
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Peracetic acid (PAA)-based pretreatment effectively improves medium-chain fatty acids (MCFAs) production from sewage sludge SCIE
期刊论文 | 2024 , 20 | ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY
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Peracetic acid (PAA), known for its environmentally friendly properties as a oxidant and bactericide, is gaining prominence in decontamination and disinfection applications. The primary product of PAA oxidation is acetate that can serve as an electron acceptor (EA) for the biosynthesis of medium-chain fatty acids (MCFAs) via chain elongation (CE) reactions. Hence, PAA-based pretreatment is supposed to be beneficial for MCFAs production from anaerobic sludge fermentation, as it could enhance organic matter availability, suppress competing microorganisms and furnish EA by providing acetate. However, such a hypothesis has rarely been proved. Here we reveal that PAA-based pretreatment leads to sig-nificant exfoliation of extracellular polymeric substances (EPS) from sludge flocs and disruption of proteinic secondary structures, through inducing highly active free radicals and singlet oxygen. The production of MCFAs increases substantially to 11,265.6 mg COD L-1 , while the undesired byproducts, specifically long-chain alcohols (LCAs), decrease to 723.5 mg COD L-1. Microbial activity tests further demonstrate that PAA pretreatment stimulates the CE process, attributed to the up-regulation of func-tional genes involved in fatty acid biosynthesis pathway. These comprehensive findings provide insights into the effectiveness and mechanisms behind enhanced MCFAs production through PAA-based tech-nology, advancing our understanding of sustainable resource recovery from sewage sludge.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of Chinese Society for Environmental Sciences, Harbin Institute of Technology, Chinese Research Academy of Environmental Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Keyword :

Extracellular polymeric substances (EPS) Extracellular polymeric substances (EPS) Medium -chain fatty acids (MCFAs) Medium -chain fatty acids (MCFAs) Metabolic activity Metabolic activity Peracetic acid (PAA) Peracetic acid (PAA) Sewage sludge Sewage sludge

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GB/T 7714 Wang, Yufen , Guo, Haixiao , Li, Xuecheng et al. Peracetic acid (PAA)-based pretreatment effectively improves medium-chain fatty acids (MCFAs) production from sewage sludge [J]. | ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY , 2024 , 20 .
MLA Wang, Yufen et al. "Peracetic acid (PAA)-based pretreatment effectively improves medium-chain fatty acids (MCFAs) production from sewage sludge" . | ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY 20 (2024) .
APA Wang, Yufen , Guo, Haixiao , Li, Xuecheng , Chen, Xueming , Peng, Lai , Zhu, Tingting et al. Peracetic acid (PAA)-based pretreatment effectively improves medium-chain fatty acids (MCFAs) production from sewage sludge . | ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY , 2024 , 20 .
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Competitive enrichment of comammox Nitrospira in floccular sludge SCIE
期刊论文 | 2024 , 251 | WATER RESEARCH
WoS CC Cited Count: 1
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The discovery of complete ammonium oxidation (comammox) has subverted the traditional perception of twostep nitrification, which plays a key role in achieving biological nitrogen removal from wastewater. Floccular sludge-based treatment technologies are being applied at the majority of wastewater treatment plants in service where detection of various abundances and activities of comammox bacteria have been reported. However, limited efforts have been made to enrich and subsequently characterize comammox bacteria in floccular sludge. To this end, a lab-scale sequencing batch reactor (SBR) in the step-feeding mode was applied in this work to enrich comammox bacteria through controlling appropriate operational conditions (dissolved oxygen of 0.5 +/- 0.1 g-O2/m3, influent ammonium of 40 g-N/m3 and uncontrolled longer sludge retention time). After 215-d operation, comammox bacteria gradually gained competitive advantages over counterparts in the SBR with a stable nitrification efficiency of 92.2 +/- 2.2 %: the relative abundance of Nitrospira reached 42.9 +/- 1.3 %, which was 13 times higher than that of Nitrosomonas, and the amoA gene level of comammox bacteria increased to 7.7 +/- 2.1 x 106 copies/g-biomass, nearly 50 times higher than that of conventional ammonium-oxidizing bacteria. The enrichment of comammox bacteria, especially Clade A Candidatus Nitrospira nitrosa, in the floccular sludge led to (i) apparent affinity constants for ammonium and oxygen of 3.296 +/- 0.989 g-N/m3 and 0.110 +/- 0.004 g-O2/m3, respectively, and (ii) significantly low N2O and NO production, with emission factors being 0.136 +/- 0.026 % and 0.023 +/- 0.013 %, respectively.

Keyword :

Complete ammonium oxidation (comammox) Complete ammonium oxidation (comammox) Floccular sludge Floccular sludge Nitrous oxide(N2O) Nitrous oxide(N2O) Sequencing batch reactor (SBR) Sequencing batch reactor (SBR)

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GB/T 7714 Hou, Jiaying , Zhu, Ying , Liu, Jinzhong et al. Competitive enrichment of comammox Nitrospira in floccular sludge [J]. | WATER RESEARCH , 2024 , 251 .
MLA Hou, Jiaying et al. "Competitive enrichment of comammox Nitrospira in floccular sludge" . | WATER RESEARCH 251 (2024) .
APA Hou, Jiaying , Zhu, Ying , Liu, Jinzhong , Lin, Limin , Zheng, Min , Yang, Linyan et al. Competitive enrichment of comammox Nitrospira in floccular sludge . | WATER RESEARCH , 2024 , 251 .
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Enhancing electricity-driven methanogenesis by assembling biotic-abiotic hybrid system in anaerobic membrane bioreactor SCIE
期刊论文 | 2024 , 391 | BIORESOURCE TECHNOLOGY
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Biotic-abiotic hybrid systems are promising technologies to enhance methane production in anaerobic wastewater treatment. However, the dense structure of the extracellular polymeric substances (EPS) present in anaerobic granular sludge (AGS) poses challenges with respect to the implementation of hybrid systems and efficient interspecies electron transfer. In this study, the use of AGS with a Ni/Fe layered double hydroxide@activated carbon (Ni/Fe LDH@C-AGS) was investigated in an anaerobic membrane bioreactor (AnMBR). The hybrid system showed a significant increase of 82% in methane production. Further research revealed that Ni/Fe LDH@C regulated the dense structure of EPS, stimulated the production of cytochromes, and facilitated the decomposition of nonconductive substances. Surprisingly, the hybrid system also promoted resistance to membrane fouling and extended membrane life by 81%. This study provides insights into the operation of a biotic-abiotic hybrid system by regulating the dense structure of EPS ultimately resulting in an enhanced methane production.

Keyword :

Anaerobic granular sludge Anaerobic granular sludge Extracellular polymeric substances Extracellular polymeric substances Interspecies electron transfer Interspecies electron transfer Membrane fouling Membrane fouling Methanogenesis Methanogenesis

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GB/T 7714 Jiang, Zhuwu , Tang, Yi , Chen, Xinyan et al. Enhancing electricity-driven methanogenesis by assembling biotic-abiotic hybrid system in anaerobic membrane bioreactor [J]. | BIORESOURCE TECHNOLOGY , 2024 , 391 .
MLA Jiang, Zhuwu et al. "Enhancing electricity-driven methanogenesis by assembling biotic-abiotic hybrid system in anaerobic membrane bioreactor" . | BIORESOURCE TECHNOLOGY 391 (2024) .
APA Jiang, Zhuwu , Tang, Yi , Chen, Xinyan , Chen, Xueming , Wang, Haoshuai , Zhang, Hongyu et al. Enhancing electricity-driven methanogenesis by assembling biotic-abiotic hybrid system in anaerobic membrane bioreactor . | BIORESOURCE TECHNOLOGY , 2024 , 391 .
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Impact of hydrogen sulfide on anammox and nitrate/nitrite-dependent anaerobic methane oxidation coupled technologies SCIE
期刊论文 | 2024 , 257 | WATER RESEARCH
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The coupling between anammox and nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) has been considered a sustainable technology for nitrogen removal from sidestream wastewater and can be implemented in both membrane biofilm reactor (MBfR) and granular bioreactor. However, the potential influence of the accompanying hydrogen sulfide (H 2 S) in the anaerobic digestion (AD)-related methane-containing mixture on anammox/n-DAMO remains unknown. To fill this gap, this work first constructed a model incorporating the C/ N/S-related bioprocesses and evaluated/calibrated/validated the model using experimental data. The model was then used to explore the impact of H2S on the MBfR and granular bioreactor designed to perform anammox/nDAMO at practical levels (i.e., 0-5% (v/v) and 0-40 g/S m 3 , respectively). The simulation results indicated that H2S in inflow gas did not significantly affect the total nitrogen (TN) removal of the MBfR under all operational conditions studied in this work, thus lifting the concern about applying AD-produced biogas to power up anammox/n-DAMO in the MBfR. However, the presence of H2S in the influent would either compromise the treatment performance of the granular bioreactor at a relatively high influent NH 4 + -N/NO2-N ratio (e.g., >1.0) or lead to increased energy demand associated with TN removal at a relatively low influent NH4+-N/NO2-N ratio (e. g., <0.7). Such a negative effect of the influent H 2 S could not be attenuated by regulating the hydraulic residence time and should therefore be avoided when applying the granular bioreactor to perform anammox/n-DAMO in practice.

Keyword :

Anammox Anammox Granular bioreactor Granular bioreactor Hydrogen sulfide(H2S) Hydrogen sulfide(H2S) Membrane biofilm reactor (MBfR) Membrane biofilm reactor (MBfR) Nitrate/nitrite-dependent anaerobic methane Nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) oxidation (n-DAMO)

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GB/T 7714 Chen, Xueming , Chen, Siying , Chen, Xinyan et al. Impact of hydrogen sulfide on anammox and nitrate/nitrite-dependent anaerobic methane oxidation coupled technologies [J]. | WATER RESEARCH , 2024 , 257 .
MLA Chen, Xueming et al. "Impact of hydrogen sulfide on anammox and nitrate/nitrite-dependent anaerobic methane oxidation coupled technologies" . | WATER RESEARCH 257 (2024) .
APA Chen, Xueming , Chen, Siying , Chen, Xinyan , Tang, Yi , Nie, Wen-Bo , Yang, Linyan et al. Impact of hydrogen sulfide on anammox and nitrate/nitrite-dependent anaerobic methane oxidation coupled technologies . | WATER RESEARCH , 2024 , 257 .
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