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学者姓名：崔明娟
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Abstract ：
Microbial-induced carbonate precipitation (MICP) technique has the potential to be an eco-friendly and sustainable solution for engineering problems. Despite the extensive amount of research that has been conducted recently on the MICP technique, there are few studies on the constitutive model of MICP-treated specimens. In this study, the statistical damage constitutive model of MICP-treated specimens was established based on the statistical theory and damage mechanics theory. The proposed model assumed that the microelement strength of biocemented sand follows the lognormal distribution and the Drucker-Prager criterion. The parameters S0 and F0 in the constitutive model were determined, and their physical significance was then discussed. The reasonableness of the proposed model was verified by comparing the theoretical results and the experimental results. The evolution of the damage variable (D), parameter S0, and parameter F0 with different calcium carbonate content (CCC) was analyzed. The statistical damage model based on the lognormal distribution was then compared with that based on the Weibull distribution. The results show that the parameter F0 and S0 can reflect the limiting strength and brittleness of MICP-treated specimens.. The specimens with higher cementation tend to have a higher accelerated damage rate. The damage variables eventually reach a stable value as the axial deformation increases. The proposed model can reflect the strain softening and strain hardening phenomena well, which can also represent the shear expansion and shear contraction characteristics of the volume strain curve. Overall, the research in this study can provide some theoretical support for the engineering application of MICP-treated specimens.
Keyword ：
Calcium carbonate content Calcium carbonate content Damage intrinsic model Damage intrinsic model Drucker-Prager criterion Drucker-Prager criterion Lognormal distribution Lognormal distribution Microbial-induced carbonate precipitation (MICP) Microbial-induced carbonate precipitation (MICP)
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| GB/T 7714 | Jiang, Qiwu , Huang, Ming , Xu, Kai et al. Statistical damage constitutive model of MICP-treated specimens based on lognormal distribution [J]. | ACTA GEOTECHNICA , 2025 , 20 (4) : 1759-1775 . |
| MLA | Jiang, Qiwu et al. "Statistical damage constitutive model of MICP-treated specimens based on lognormal distribution" . | ACTA GEOTECHNICA 20 . 4 (2025) : 1759-1775 . |
| APA | Jiang, Qiwu , Huang, Ming , Xu, Kai , Cui, Mingjuan , Li, Shuang , Jin, Guixiao . Statistical damage constitutive model of MICP-treated specimens based on lognormal distribution . | ACTA GEOTECHNICA , 2025 , 20 (4) , 1759-1775 . |
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Abstract ：
Enzyme-induced carbonate precipitation (EICP) has emerged as an innovative soil stabilization technology to precipitate CaCO3 by catalyzing urea decomposition. Although extensive efforts have been made to increase the calcium carbonate content (CCC) formed in the EICP process for the better bio-cementation effect, the cementability and micromechanical properties of CaCO3 are rarely known. A study of the cementitious characteristics and micromechanical properties of CaCO3 precipitates with different mixing percentages of crystal morphology is essential for soil improvement. In the present study, ultrasonic oscillation tests and nanoindentation tests were performed to investigate the cementability and micromechanical properties of CaCO3 precipitate. The results show that the cementability and micromechanical properties of CaCO3 precipitate are related to the composition of the crystal morphology. A high content of calcite is beneficial to improve the adhesion of calcium carbonate precipitate. Calcite has better mechanical properties (elastic modulus, hardness and ductility) than vaterite, and the presence of vaterite can significantly affect the measured value of mechanical properties in nanoindentation tests. The ductility of CaCO3 precipitate induced by crude soybean urease (CSU) is higher than that of CaCO3 precipitate induced by commercially available pure enzyme, suggesting that commercially available pure enzyme can be replaced by CSU for cost-effective field-scale engineering applications. This work can provide insight into optimizing the properties of CaCO3 precipitate from the micro-scale. © 2024 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences
Keyword ：
Cementability Cementability Enzyme-induced carbonate precipitation (EICP) Enzyme-induced carbonate precipitation (EICP) Micromechanical properties Micromechanical properties Nanoindentation tests Nanoindentation tests
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| GB/T 7714 | Xu, K. , Huang, M. , Cui, M. et al. Effect of crystal morphology on cementability and micromechanical properties of calcium carbonate precipitate induced by crude soybean enzyme [J]. | Journal of Rock Mechanics and Geotechnical Engineering , 2024 , 16 (12) : 5095-5108 . |
| MLA | Xu, K. et al. "Effect of crystal morphology on cementability and micromechanical properties of calcium carbonate precipitate induced by crude soybean enzyme" . | Journal of Rock Mechanics and Geotechnical Engineering 16 . 12 (2024) : 5095-5108 . |
| APA | Xu, K. , Huang, M. , Cui, M. , Li, S. . Effect of crystal morphology on cementability and micromechanical properties of calcium carbonate precipitate induced by crude soybean enzyme . | Journal of Rock Mechanics and Geotechnical Engineering , 2024 , 16 (12) , 5095-5108 . |
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Abstract ：
The thermal conductivity of backfill materials directly affects the heat transfer efficiency between energy geo-structures and the surrounding stratum. Microbially induced carbonate precipitation (MICP) possesses great potential for improving the thermal conductivity of backfill materials. Magnetic iron oxide nanoparticles (i.e., nano-Fe3O4) have been proven to enhance bacterial biochemical activity by altering the permeability of bacterial biofilms, thus potentially improving the MICP process. It was supposed to enhance the thermal conductivity of backfill materials, allowing for applying energy geo-structures in arid environments. In this study, MICP in a solution environment was conducted to analyze bacterial urease activity and morphology of precipitation at varying nano-Fe3O4 contents. Additionally, sand columns treated with MICP and different nano-Fe3O4 contents were performed to obtain the thermal conductivity and unconfined compressive strength (UCS) through the transient plane source (TPS) method and uniaxial compression (UC) experiment. The mineral type, precipitation morphology, and microstructure were identified using scanning electron microscopy (SEM) and X-ray diffraction (XRD). The mechanism of the effect of nano-Fe3O4 on bacterial urease activity and thermal-mechanical behaviors was also discussed. The results indicated that the nano-Fe3O4 could enhance bacterial urease activity and promote vaterite precipitation in the solution environment. Conversely, when applied to MICP-treated sand, nano-Fe3O4 could facilitate calcite formation. Increasing the nano-Fe3O4 content showed a positive correlation with increased thermal conductivity and UCS. Specifically, the optimal values of thermal conductivity and UCS increased by 2.42 times and 2.39 times, respectively, compared to MICP-treated specimens without nano-Fe3O4. Microstructure analysis revealed that calcite precipitation at the particle contact served a dual function: cementing particles, thereby improving the mechanical strength and simultaneously acting as a “thermal bridge” to enhance the thermal conductivity. Furthermore, this study provides a new perspective on utilizing magnetized bacteria to reinforce specific locations within rocks and soils in the presence of an external magnetic field. © 2024 Elsevier Ltd
Keyword ：
Bacterial urease activity Bacterial urease activity Crystal morphology Crystal morphology Geothermal energy Geothermal energy Magnetic iron oxide nanoparticles (nano-Fe3O4) Magnetic iron oxide nanoparticles (nano-Fe3O4) Microbially induced carbonate precipitation (MICP) Microbially induced carbonate precipitation (MICP) Thermal conductivity Thermal conductivity
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| GB/T 7714 | Li, S. , Huang, M. , Cui, M. et al. Improving the thermal-mechanical performance of bio-treated backfill materials by addition of magnetic iron oxide nanoparticles (nano-Fe3O4) [J]. | Geomechanics for Energy and the Environment , 2024 , 39 . |
| MLA | Li, S. et al. "Improving the thermal-mechanical performance of bio-treated backfill materials by addition of magnetic iron oxide nanoparticles (nano-Fe3O4)" . | Geomechanics for Energy and the Environment 39 (2024) . |
| APA | Li, S. , Huang, M. , Cui, M. , Jin, G. , Xu, K. . Improving the thermal-mechanical performance of bio-treated backfill materials by addition of magnetic iron oxide nanoparticles (nano-Fe3O4) . | Geomechanics for Energy and the Environment , 2024 , 39 . |
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Abstract ：
Microbially or enzyme induced carbonate precipitation has emerged to be a new type of soil improvement method. However, it appears that the biocementation process is affected by many factors and a common understanding on the control factors on the biocement effect has not been reached. This paper attempts to identify the main factors that controlling the MICP or EICP effect through an in-depth discussion on the fundamentals of biocementation process. Similar to other cemented granular materials, biocemented soil is a structural soil composite consisting of soil skeleton and biocement force chain or biocement network. The strength and stiffness of the biocemented soil is controlled by the reinforcement effect of the biocement network on the soil skeleton or the interplay of the soil skeleton and precipitates. The contribution of the strength by soil skeleton is affected by the soil types and soil properties, while the contribution of the precipitates is through the distribution of the biocement network and the properties of the precipitates. © 2024
Keyword ：
Biocementation Biocementation Influencing factor Influencing factor Mechanism Mechanism Strength enhancement Strength enhancement
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| GB/T 7714 | Lai, H. , Ding, X. , Cui, M. et al. Factors affecting the effectiveness of biocementation of soil [J]. | Biogeotechnics , 2024 , 2 (3) . |
| MLA | Lai, H. et al. "Factors affecting the effectiveness of biocementation of soil" . | Biogeotechnics 2 . 3 (2024) . |
| APA | Lai, H. , Ding, X. , Cui, M. , Zheng, J. , Chu, J. , Chen, Z. . Factors affecting the effectiveness of biocementation of soil . | Biogeotechnics , 2024 , 2 (3) . |
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Calcium salt is an important contributing factor for calcium-based biomineralization.To study the effect of calcium salt on soil biomineralization using crude soybean urease,the calcium salts,including the calcium chloride(CaCl2),calcium acetate((CH3COO)2Ca)and calcium nitrate(Ca(NO3)2),were used to prepare the biotreatment solution to carry out the biomineralization tests in this paper.Two series of biomineralization tests in solution and sand column,respectively,were conducted.Scanning electron microscopy(SEM)and X-ray diffraction(XRD)were performed to determine the microscopic charac-teristics of the precipitated calcium carbonate(CaCO3)crystals.The experimental results indicate that the biomineralization effect is the best for the CaCl2 case,followed by(CH3COO)2Ca,and worst for Ca(NO3)2 under the test conditions of this study(i.e.1 mol/L of calcium salt-urea).The mechanism for the effect of the calcium salt on the biomineralization of crude soybean urease mainly involves:(1)inhibition of urease activity,and(2)influence on the crystal size and morphology of CaCO3.Besides Ca2+,the anions in solution can inhibit the activity of crude soybean urease,and NO3 has a stronger inhibitory effect on the urease activity compared with both CH3COO-and Cl-.The co-inhibition of Ca2+and NO3 on the activity of urease is the key reason for the worst biomineralization of the Ca(NO3)2 case in this study.The dif-ference in biomineralization between the CaCl2 and(CH3COO)2Ca cases is strongly correlated with the crystal morphology of the precipitated CaCO3.
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| GB/T 7714 | Yajie Weng , Junjie Zheng , Hanjiang Lai et al. Biomineralization of soil with crude soybean urease using different calcium salts [J]. | 岩石力学与岩土工程学报（英文版） , 2024 , 16 (5) : 1788-1798 . |
| MLA | Yajie Weng et al. "Biomineralization of soil with crude soybean urease using different calcium salts" . | 岩石力学与岩土工程学报（英文版） 16 . 5 (2024) : 1788-1798 . |
| APA | Yajie Weng , Junjie Zheng , Hanjiang Lai , Mingjuan Cui , Xingzhi Ding . Biomineralization of soil with crude soybean urease using different calcium salts . | 岩石力学与岩土工程学报（英文版） , 2024 , 16 (5) , 1788-1798 . |
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Abstract ：
Calcium salt is an important contributing factor for calcium-based biomineralization. To study the effect of calcium salt on soil biomineralization using crude soybean urease, the calcium salts, including the calcium chloride (CaCl2), calcium acetate ((CH3COO)(2)Ca) and calcium nitrate (Ca(NO3)(2)), were used to prepare the biotreatment solution to carry out the biomineralization tests in this paper. Two series of biomineralization tests in solution and sand column, respectively, were conducted. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were performed to determine the microscopic characteristics of the precipitated calcium carbonate (CaCO3) crystals. The experimental results indicate that the biomineralization effect is the best for the CaCl2 case, followed by (CH3COO)(2)Ca, and worst for Ca(NO3)(2) under the test conditions of this study (i.e. 1 mol/L of calcium salt-urea). The mechanism for the effect of the calcium salt on the biomineralization of crude soybean urease mainly involves: (1) inhibition of urease activity, and (2) influence on the crystal size and morphology of CaCO3. Besides Ca2+, the anions in solution can inhibit the activity of crude soybean urease, and NO3- has a stronger inhibitory effect on the urease activity compared with both CH3COO- and Cl-. The co-inhibition of C-a2+ and NO3- on the activity of urease is the key reason for the worst biomineralization of the Ca(NO3)(2) case in this study. The difference in biomineralization between the CaCl2 and (CH3COO)2(C)a cases is strongly correlated with the crystal morphology of the precipitated CaCO3. (c) 2024 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).
Keyword ：
Biomineralization Biomineralization Calcium salt Calcium salt Crude soybean urease Crude soybean urease Influence mechanism Influence mechanism
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| GB/T 7714 | Weng, Yajie , Zheng, Junjie , Lai, Hanjiang et al. Biomineralization of soil with crude soybean urease using different calcium salts [J]. | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING , 2024 , 16 (5) : 1788-1798 . |
| MLA | Weng, Yajie et al. "Biomineralization of soil with crude soybean urease using different calcium salts" . | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING 16 . 5 (2024) : 1788-1798 . |
| APA | Weng, Yajie , Zheng, Junjie , Lai, Hanjiang , Cui, Mingjuan , Ding, Xingzhi . Biomineralization of soil with crude soybean urease using different calcium salts . | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING , 2024 , 16 (5) , 1788-1798 . |
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Abstract ：
The one-phase-low-pH method is a simple, efficient, and user-friendly biogrouting technique that can effectively improve the biomineralization of enzyme-induced carbonate precipitation (EICP) using free urease enzyme. One of the most significant advantages of this method is its capacity to effectively delay calcium carbonate (CaCO3) precipitation by reducing the pH of the solution through the addition of acid. This prevents bioclogging during the biogrouting process and improves the biomineralization effect. However, the biomineralization of the one-phase-low-pH based EICP method may be influenced by the specific acid used. To investigate the impact of acid type on the one-phase-low-pH EICP method using crude soybean urease solution (CSUS), four types of acids, including hydrochloric acid (HCl), nitric acid (HNO3), acetic acid (CH3COOH), and lactic acid (C3H6O3), were used to adjust the pH of CSUS. A series of macroscopic and microscopic experiments were conducted to evaluate the effect of acid type on the onephase-low-pH EICP method. The results indicate that the acid has an inhibition on the urease activity (UA) of CSUS. Among the acids tested, HNO3 exhibits the most pronounced inhibitory effect on the UA of CSUS, followed by HCl, and the least pronounced inhibitory effect for CH3COOH and C3H6O3 under the same pH conditions. Meanwhile, CH3COOH and C3H6O3 could provide a longer delay duration of CaCO3 precipitation than HNO3 and HCl. Therefore, the one-phase-low-pH EICP method based on CH3COOH and C3H6O3 can significantly improve the effective biocementation depth compared to that based on HNO3 and HCl. Nevertheless, the different types of acids appear to have no obvious effect on the polymorph and crystalline of the precipitated CaCO3 crystals. (c) 2024 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).
Keyword ：
Acid type Acid type Biomineralization Biomineralization Crude soybean urease solution (CSUS) Crude soybean urease solution (CSUS) (EICP) (EICP) Enzyme-induced carbonate precipitation Enzyme-induced carbonate precipitation One-phase-low-pH method One-phase-low-pH method
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| GB/T 7714 | Weng, Yajie , Lai, Hanjiang , Zheng, Junjie et al. Effect of acid type on biomineralization of soil using crude soybean urease solution [J]. | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING , 2024 , 16 (12) : 5135-5146 . |
| MLA | Weng, Yajie et al. "Effect of acid type on biomineralization of soil using crude soybean urease solution" . | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING 16 . 12 (2024) : 5135-5146 . |
| APA | Weng, Yajie , Lai, Hanjiang , Zheng, Junjie , Cui, Mingjuan , Chen, Yihang , Xu, Zhitao et al. Effect of acid type on biomineralization of soil using crude soybean urease solution . | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING , 2024 , 16 (12) , 5135-5146 . |
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微生物诱导碳酸钙沉淀(MICP)是环境岩土工程领域一项新型的土体加固技术.微生物的生长及活性会受到外部磁场的影响,改变MICP中碳酸钙的晶型晶貌及沉淀方式,从而对碳酸钙的胶结性能产生影响.采用纳米四氧化三铁(Nano-Fe3O4),设计了Nano-Fe3O4作用下的微生物诱导碳酸钙沉淀的水溶液及MICP砂土固化试验,对比分析了Nano-Fe3O4含量对微生物诱导生成的碳酸钙晶体含量(CCC)、类型、比例以及MICP固化砂土力学强度等参数的影响规律,并结合扫描电镜(SEM)试验分析了溶液环境及砂柱中碳酸钙的微观形貌特征,系统归纳了Nano-Fe3O4对MICP的作用效果及机制.结果表明:(1)Nano-Fe3O4能够有效改善细菌的新陈代谢性能,显著提高细菌OD600及脲酶活性;(2)溶液环境中,MICP产生的碳酸钙晶体类型以球霰石为主,含少量方解石,且Nano-Fe3O4含量增加能够促进球霰石的生成及增大MICP沉淀物中稳定相碳酸钙所占的比例;(3)Nano-Fe3O4可以显著提高MICP固化砂土的无侧限抗压强度和CCC;(4)SEM分析结果表明,溶液环境中,碳酸钙晶体以球型堆积为主,MICP固化砂柱中碳酸钙晶型随Nano-Fe3O4含量的增加逐渐呈菱柱状堆积.
Keyword ：
MICP固化砂土 MICP固化砂土 微生物诱导碳酸钙沉淀(MICP) 微生物诱导碳酸钙沉淀(MICP) 晶体类型与形貌 晶体类型与形貌 溶液环境 溶液环境 纳米四氧化三铁 纳米四氧化三铁 细菌脲酶活性 细菌脲酶活性
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| GB/T 7714 | 李爽 , 黄明 , 崔明娟 et al. 纳米四氧化三铁对微生物诱导碳酸钙沉淀的作用效果与机理研究 [J]. | 材料导报 , 2024 , 38 (20) : 76-83 . |
| MLA | 李爽 et al. "纳米四氧化三铁对微生物诱导碳酸钙沉淀的作用效果与机理研究" . | 材料导报 38 . 20 (2024) : 76-83 . |
| APA | 李爽 , 黄明 , 崔明娟 , 胡鑫杭 , 许凯 , 姜启武 . 纳米四氧化三铁对微生物诱导碳酸钙沉淀的作用效果与机理研究 . | 材料导报 , 2024 , 38 (20) , 76-83 . |
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Bacterial suspension is an essential component of microbially induced carbonate precipitation (MICP)based biocement and a large-scale production is required for field applications. In this study, a new bacterial concentration method is proposed to enable high concentration bacterial suspension to be produced to facilitate field work. By adding low concentration calcium to bacterial suspension, flocs are formed and bacterial cells are adsorbed on the flocs to achieve bacterial concentration. Compared to the traditional bacterial concentration method using centrifugation and freezing-drying method, the proposed method can concentrate a large volume of bacterial suspension without using special equipment. The feasibility of this method is verified by bacterial concentration tests, solution tests and sand column treatment tests. The results of both the solution test and the sand column treatment test show that the bacterial suspension concentrated by the proposed method can be effectively used for soil biocementation. There is a threshold calcium concentration that allows a complete bacterial concentration for the proposed method, and this threshold calcium concentration tends to increase linearly with the optical density of the cell suspension at a wavelength of 600 nm (OD600). (c) 2024 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).
Keyword ：
Bacterial concentration Bacterial concentration Biocement Biocement Biocementation Biocementation (MICP) (MICP) Microbially induced carbonate precipitation Microbially induced carbonate precipitation
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| GB/T 7714 | Lai, Hanjiang , Ding, Xingzhi , Cui, Mingjuan et al. A new bacterial concentration method for large-scale applications of biomineralization [J]. | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING , 2024 , 16 (12) : 5109-5120 . |
| MLA | Lai, Hanjiang et al. "A new bacterial concentration method for large-scale applications of biomineralization" . | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING 16 . 12 (2024) : 5109-5120 . |
| APA | Lai, Hanjiang , Ding, Xingzhi , Cui, Mingjuan , Zheng, Junjie , Chu, Jian , Chen, Zhibo et al. A new bacterial concentration method for large-scale applications of biomineralization . | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING , 2024 , 16 (12) , 5109-5120 . |
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目前关于微生物固化体的本构模型研究较少,本文基于Lemaitre应变等价性理论的损伤模型,假定微生物固化体的微元强度服从对数正态随机分布,假设微元破坏符合D-P准则,建立MICP固化钙质砂的统计损伤本构模型.根据试验数据确定本构模型中的参数S.和参数F0,并对其物理意义进行讨论,将该模型理论结果与试验结果进行对比,验证了该模型的合理性,最后分析了损伤变量D,参数S0和参数F0随碳酸钙含量变化的演化规律.研究结果表明:参数F0反映微生物固化钙质砂的极限强度特性,参数S.代表其脆性和延性特征;胶结水平越高,损伤速度越快,损伤值越大,随轴向变形持续增大,损伤变量最终会趋于稳定;该模型能够较好地模拟应变软化和应变硬化现象,同时可以反映体积应变曲线的剪胀和剪缩特性.本研究成果可为MICP固化土体的工程应用提供一定的理论基础.
Keyword ：
D-P准则 D-P准则 对数正态分布 对数正态分布 微生物固化体 微生物固化体 损伤本构模型 损伤本构模型 钙质砂 钙质砂
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| GB/T 7714 | 姜启武 , 黄明 , 许凯 et al. MICP固化钙质砂的统计损伤本构模型 [J]. | 工程地质学报 , 2024 , 32 (5) : 1526-1535 . |
| MLA | 姜启武 et al. "MICP固化钙质砂的统计损伤本构模型" . | 工程地质学报 32 . 5 (2024) : 1526-1535 . |
| APA | 姜启武 , 黄明 , 许凯 , 崔明娟 . MICP固化钙质砂的统计损伤本构模型 . | 工程地质学报 , 2024 , 32 (5) , 1526-1535 . |
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