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学者姓名：姚爱芳
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Background/Objectives: The clinical potential of antimicrobial peptides (AMPs) against dual threats like antimicrobial resistance (AMR) and cancer is often limited by their high host cell toxicity. Here, we focused on brevinin-2OS (B2OS), a novel peptide from the skin of Odorrana schmackeri with potent haemolytic activity. The objective was to study the structure-activity relationship and optimise the safety via targeted modifications. Methods: A dual-modification strategy involving C-terminal truncation and subsequent N-terminal D-amino acid substitution was employed. The bioactivities and safety profiles of the resulting analogues were evaluated using antimicrobial, haemolysis, and cytotoxicity assays. Result: Removal of the rana box in B2OS(1-22)-NH2 substantially reduced haemolysis while maintaining bioactivities. Remarkably, the D-leucine substitution in [D-Leu2]B2OS(1-22)-NH2 displayed a superior HC50 value of 118.1 mu M, representing a more than ten-fold improvement compared to its parent peptide (HC50 of 10.44 mu M). This optimised analogue also demonstrated faster bactericidal kinetics and enhanced membrane permeabilisation, leading to a greater than 22-fold improvement in its therapeutic index against Gram-positive bacteria. Conclusions: The C-terminal rana box is a primary determinant of toxicity rather than a requirement for activity in the B2OS scaffold. The engineered peptide [D-Leu2]B2OS(1-22)-NH2 emerges as a promising lead compound, and this dual-modification strategy provides a powerful design principle for developing safer, more effective peptide-based therapeutics.
Keyword ：
antimicrobial peptide antimicrobial peptide brevinin-2 brevinin-2 peptide modification peptide modification structure-activity relationship structure-activity relationship therapeutic index therapeutic index
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| GB/T 7714 | Yao, Aifang , Zhang, Zeyu , Song, Zhengmin et al. Rational Engineering of a Brevinin-2 Peptide: Decoupling Potency from Toxicity Through C-Terminal Truncation and N-Terminal Chiral Substitution [J]. | ANTIBIOTICS-BASEL , 2025 , 14 (8) . |
| MLA | Yao, Aifang et al. "Rational Engineering of a Brevinin-2 Peptide: Decoupling Potency from Toxicity Through C-Terminal Truncation and N-Terminal Chiral Substitution" . | ANTIBIOTICS-BASEL 14 . 8 (2025) . |
| APA | Yao, Aifang , Zhang, Zeyu , Song, Zhengmin , Yuan, Yi , Chen, Xiaoling , Ma, Chengbang et al. Rational Engineering of a Brevinin-2 Peptide: Decoupling Potency from Toxicity Through C-Terminal Truncation and N-Terminal Chiral Substitution . | ANTIBIOTICS-BASEL , 2025 , 14 (8) . |
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Eutectogels are emerging as soft material with great promise for application in soft electronics. However, they are generally unable to maintain autonomous self-healing and adhesiveness in submerged and humid environments, which causes some limitations to their applicability. Moreover, they often fail to undergo closed-loop recycling, due to the general presence of chemical cross-linking. Here, a amphiphilic eutectogel synthesized via a one-step photopolymerization strategy within a tailored deep eutectic solvent (DES) system, employing purely physical cross-linking mechanisms. Underwater, nanoscale phase-separated structures (similar to 14.6 nm), triggered by hydrophobic aggregation in solution, arise from dominant hydrophobic interactions among polymer chains. These distinct domains facilitate efficient water drainage at the gel-substrate interface and impart excellent water resistance. The resulting eutectogel exhibits autonomous self-healing and robust adhesion in both air and underwater environments. The eutectogel could achieve closed-loop recycling without affecting their mechanical properties and adhesive performance. By finely tuning the dynamic interactions between polymer chains and DES, the gel achieves a physically crosslinked polymeric network with enhanced mechanical resilience and environmental stability across broad temperature ranges. The DES acts as a medium for monomer polymerization, facilitating synergistic interactions and homogeneous phase distribution, enabling synergistic interactions between hydrophilic and hydrophobic monomers, offering a universal strategy for eutectogel fabrication. The presence of freely mobile ions allows the gel to function as both a strain and pressure sensor in air and underwater, including applications in human motion sensing and information transmission, highlighting its immense potential for multifunctional sensing and wearable systems.
Keyword ：
Eutectogels Eutectogels Nanoscale phase-separated Nanoscale phase-separated Recyclability Recyclability Underwater adhesive Underwater adhesive Underwater self-healing Underwater self-healing Underwater sensing Underwater sensing
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| GB/T 7714 | Zhang, Zeyu , Yao, Aifang , Raffa, Patrizio . Physically crosslinked amphiphilic eutectogels with underwater self-healing, strong adhesion, environmental stability and closed-loop recyclability for underwater sensing and information transmission [J]. | CHEMICAL ENGINEERING JOURNAL , 2025 , 522 . |
| MLA | Zhang, Zeyu et al. "Physically crosslinked amphiphilic eutectogels with underwater self-healing, strong adhesion, environmental stability and closed-loop recyclability for underwater sensing and information transmission" . | CHEMICAL ENGINEERING JOURNAL 522 (2025) . |
| APA | Zhang, Zeyu , Yao, Aifang , Raffa, Patrizio . Physically crosslinked amphiphilic eutectogels with underwater self-healing, strong adhesion, environmental stability and closed-loop recyclability for underwater sensing and information transmission . | CHEMICAL ENGINEERING JOURNAL , 2025 , 522 . |
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Hydrogel-based smart windows have the potential to reduce the energy consumption associated with air conditioning and lighting systems. Most existing hydrogels exhibit a unidirectional response to a specific temperature, limiting their applicability. In this work, a bidirectional temperature-responsive hydrogel was developed by incorporating hydroxypropyl cellulose (HPC) into a physically cross-linked copolymer matrix of N-(2-hydroxyethyl) acrylamide (HEAA) and acrylamide (AM), with cetyltrimethylammonium bromide (CTAB) used to stabilize lauryl methacrylate (LMA) micelles in a deep eutectic solvent (DES)/H2O binary solvent system. At higher temperatures, the hydrogel becomes opaque through phase separation driven by the lower critical solution temperature (LCST) of HPC. At lower temperatures, another optical transition seems to be governed by the growth of micelles formed from LMA. The temperature operating window can be tuned by changing the composition, keeping a rapid optical switching response of less than 30 s. Furthermore, due to the dynamic reversibility of hydrophobic associations and hydrogen bonding, the hydrogel exhibits excellent mechanical strength and self-healing capability at room temperature. The presence of the DES also contributes to its antifreezing performance, allowing the hydrogel to retain flexibility even at -20 degrees C. With its integrated functionalities, this material represents a highly promising candidate for smart window applications in real-world environments.
Keyword ：
antifreezing antifreezing hydrogels hydrogels self-healing self-healing smart window smart window thermoresponsive thermoresponsive
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| GB/T 7714 | Zhang, Zeyu , Yao, Aifang , Cheng, Zao et al. Thermoresponsive Physically Cross-Linked Hydrogels with Bidirectional Optical Response for Smart Windows Application [J]. | ACS APPLIED MATERIALS & INTERFACES , 2025 . |
| MLA | Zhang, Zeyu et al. "Thermoresponsive Physically Cross-Linked Hydrogels with Bidirectional Optical Response for Smart Windows Application" . | ACS APPLIED MATERIALS & INTERFACES (2025) . |
| APA | Zhang, Zeyu , Yao, Aifang , Cheng, Zao , Raffa, Patrizio . Thermoresponsive Physically Cross-Linked Hydrogels with Bidirectional Optical Response for Smart Windows Application . | ACS APPLIED MATERIALS & INTERFACES , 2025 . |
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Antimicrobial peptides (AMPs) that exert multiple functions are considered promising candidates to combat the bacterial drug resistance crisis. Nowadays, targeted peptide modification has been widely recognised to improve biological activity and make up for deficiencies in clinical applications such as toxicity. In this study, a helix-loop peptide was isolated and identified from the skin secretion of the Wuyi torrent frog Amolops wuyiensis, namely, ranatuerin-2-AW (R2AW) (GFMDTAKNVAKNVAATLLDKLKCKITGGC). Target modifications were made to R2AW to study the structure-activity relationships and to optimise its bioactivities. Five analogues were progressively designed via residue substitution and truncation and the antibacterial and anticancer activities were evaluated. We found that the serine-substitution and cyclic-domain-deletion products showed similar antibacterial activity to the natural peptide R2AW, implying that the disulphide bridge and Rana box were dispensable for the antibacterial activity of ranatuerin-2 peptides. Notably, the cationicity- and hydrophobicity-enhanced variant, [Lys(4,19), Leu(20)]R2AW(1-22)-NH2, exhibited significantly optimised antibacterial and anticancer activities. Additionally, it killed bacteria by membrane disruption at a highly efficient rate. Moreover, [Lys(4,19), Leu(20)]R2AW(1-22)-NH2 exerted potential in vivo efficacy in a methicillin-resistant Staphylococcus aureus (MRSA)-infected waxworm model. Overall, this study demonstrated some rational design ideas for optimising the dual antibacterial and anticancer activities of ranatuerin-2 peptides and it proposes [Lys(4,19), Leu(20)]R2AW(1-22)-NH2 as an appealing candidate for therapeutic development.
Keyword ：
antimicrobial peptides (AMPs) antimicrobial peptides (AMPs) drug optimisation drug optimisation multidrug-resistant bacteria multidrug-resistant bacteria peptide design peptide design ranatuerin ranatuerin
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| GB/T 7714 | Yao, Aifang , Liu, Tianxing , Cai, Yuhai et al. Progressive Design of a Ranatuerin-2 Peptide from Amolops wuyiensis: Enhancement of Bioactivity and In Vivo Efficacy [J]. | ANTIBIOTICS-BASEL , 2024 , 13 (1) . |
| MLA | Yao, Aifang et al. "Progressive Design of a Ranatuerin-2 Peptide from Amolops wuyiensis: Enhancement of Bioactivity and In Vivo Efficacy" . | ANTIBIOTICS-BASEL 13 . 1 (2024) . |
| APA | Yao, Aifang , Liu, Tianxing , Cai, Yuhai , Zhou, Siqi , Chen, Xiaoling , Zhou, Mei et al. Progressive Design of a Ranatuerin-2 Peptide from Amolops wuyiensis: Enhancement of Bioactivity and In Vivo Efficacy . | ANTIBIOTICS-BASEL , 2024 , 13 (1) . |
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Conductive hydrogels have emerged as fascinating materials for flexible electronics because of their integrated conductivity, mechanical flexibility, and the possibility to introduce several smart functions. However, the swelling of hydrogels in aqueous environments significantly reduces their applicability where contact with water is unavoidable. In this study, a physically cross-linked composite hydrogel is proposed, that is transparent, highly stretchable, anti-swelling, capable of autonomous self-healing, adhesive, and anti-freezing. The hydrogel is synthesized through a simple one-step photopolymerization in a novel deep eutectic solvent (DES)/water system. Dynamic physical interactions, including hydrophobic interaction, hydrogen bonding, and electrostatic interactions, confer remarkable transparency (92%), self-healing capability (up to 94%), good adhesion to a wide array of substrates (91 to 199 kPa), high toughness (1.46 MJ m-3), excellent elongation at break (up to 2064%), and resistance to swelling in water (equilibrium swelling ratio of 3% in water for 30 days) even in solutions at different pH (pH 1-11), and in other solvents. The incorporation of a DES contributes to exceptional anti-freezing performance. The transparent sensor achieves multifunctional sensing and human motion detection with high sensitivity and stability. Notably, the sensor demonstrates information transmission underwater through stretching and pressing, showcasing its immense potential in underwater flexible devices. In this study, a physically cross-linked composite hydrogel is proposed, that is transparent, highly stretchable, anti-swelling, capable of autonomous self-healing, adhesive, and anti-freezing. The hydrogel is synthesized through a simple one-step photopolymerization, and its use as multi-functional flexible sensor, capable of detecting a series of human movements, and also suitable for underwater communication is demonstrated. image
Keyword ：
conductive hydrogels conductive hydrogels flexible electronics flexible electronics self-healing self-healing swelling-resistant swelling-resistant underwater sensing underwater sensing
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| GB/T 7714 | Zhang, Zeyu , Yao, Aifang , Raffa, Patrizio . Transparent, Highly Stretchable, Self-Healing, Adhesive, Freezing-Tolerant, and Swelling-Resistant Multifunctional Hydrogels for Underwater Motion Detection and Information Transmission [J]. | ADVANCED FUNCTIONAL MATERIALS , 2024 , 34 (49) . |
| MLA | Zhang, Zeyu et al. "Transparent, Highly Stretchable, Self-Healing, Adhesive, Freezing-Tolerant, and Swelling-Resistant Multifunctional Hydrogels for Underwater Motion Detection and Information Transmission" . | ADVANCED FUNCTIONAL MATERIALS 34 . 49 (2024) . |
| APA | Zhang, Zeyu , Yao, Aifang , Raffa, Patrizio . Transparent, Highly Stretchable, Self-Healing, Adhesive, Freezing-Tolerant, and Swelling-Resistant Multifunctional Hydrogels for Underwater Motion Detection and Information Transmission . | ADVANCED FUNCTIONAL MATERIALS , 2024 , 34 (49) . |
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Active compounds were usually incorporated into biopolymer films to enhance their properties. The tensile strength (TS) and elongation at break (EAB) of the gelatin composite films increased along with the addition of epsilon-polylysine (epsilon-PL) and zinc oxide nanoparticles (nano-ZnO). When the concentrations of epsilon-PL and nano-ZnO were 4% and 0.5%, TS and EAB reached to the maximum which were 53.98 +/- 2.61 MPa and 16.05 +/- 1.76%. The water vapor permeability, water solubility, and water content of the composite films decreased from 2.01 +/- 0.04 10-10 g m-1 Pa-1 s-1 to 1.56 +/- 0.07 x 10-10 g m-1 Pa-1 s-1, from 45.23 +/- 1.54% to 31.39 +/- 1.24% and from 23.89 +/- 1.41% to 17.34 +/- 2.61%, respectively, compared with gelatin film. The light barrier capacity of the composite film was improved. The surface of the film was relatively smooth, and the cross-section was overall flat. FTIR results indicated that no chemical reaction occurred among epsilon-PL, nano-ZnO and gelatin. The chemical structure of the films remained unchanged throughout the heat-sealing process. Moreover, the gelatin films containing epsilon-PL and nano-ZnO showed excellent antibacterial activity. Our findings suggest that the gelatin composite films with antibacterial property hold promise for the application in food packaging materials. Zinc oxide nanoparticles (nano-ZnO) and epsilon-polylysine (epsilon-PL) were added into films. Nano-ZnO and epsilon-PL could improve mechanical and barrier properties of gelatin film. Film containing nano-ZnO and epsilon-PL showed the best antibacterial activity.image
Keyword ：
active composite film active composite film characterization characterization epsilon-polylysine epsilon-polylysine gelatin gelatin zinc oxide nanoparticles zinc oxide nanoparticles
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| GB/T 7714 | Huang, Ruyang , Yao, Aifang , Yan, Yongyong et al. Development and characterization of multifunctional fish gelatin composite films reinforced with ε-polylysine and zinc oxide nanoparticles [J]. | EFOOD , 2024 , 5 (4) . |
| MLA | Huang, Ruyang et al. "Development and characterization of multifunctional fish gelatin composite films reinforced with ε-polylysine and zinc oxide nanoparticles" . | EFOOD 5 . 4 (2024) . |
| APA | Huang, Ruyang , Yao, Aifang , Yan, Yongyong , Wang, Jingyi , Li, Qingxiang , Li, Kezhi et al. Development and characterization of multifunctional fish gelatin composite films reinforced with ε-polylysine and zinc oxide nanoparticles . | EFOOD , 2024 , 5 (4) . |
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Antimicrobial peptides have gradually attracted interest as promising alternatives to conventional agents to control the worldwide health threats posed by antibiotic resistance and cancer. Crabrolin is a tridecapeptide extracted from the venom of the European hornet (Vespa crabro). Its antibacterial and anticancer potentials have been underrated compared to other peptides discovered from natural resources. Herein, a series of analogs were designed based on the template sequence of crabrolin to study its structure-activity relationship and enhance the drug's potential by changing the number, type, and distribution of charged residues. The cationicity-enhanced derivatives were shown to have improved antibacterial and anticancer activities with a lower toxicity. Notably, the double-arginine-modified product, crabrolin-TR, possessed a potent capacity against Pseudomonas aeruginosa(minimum inhibitory concentration (MIC) = 4 mu M), which was around thirty times stronger than the parent peptide (MIC = 128 mu M). Furthermore, crabrolin-TR showed an in vivo treatment efficacy in a Klebsiella-pneumoniae-infected waxworm model and was non-toxic under its maximum MBC value (MIC = 8 mu M), indicating its therapeutic potency and better selectivity. Overall, we rationally designed functional peptides by progressively increasing the number and distribution of charged residues, demonstrating new insights for developing therapeutic molecules from natural resources with enhanced properties, and proposed crabrolin-TR as an appealing antibacterial and anticancer agent candidate for development
Keyword ：
anti-infective materials anti-infective materials antimicrobial peptides antimicrobial peptides bacterial biofilms bacterial biofilms cancer cancer crabrolin crabrolin Galleria mellonella Galleria mellonella peptide design peptide design Vespa crabro Vespa crabro
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| GB/T 7714 | Yao, Aifang , Ma, Yingxue , Sun, Ruize et al. A Designed Analog of an Antimicrobial Peptide, Crabrolin, Exhibits Enhanced Anti-Proliferative and In Vivo Antimicrobial Activity [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (19) . |
| MLA | Yao, Aifang et al. "A Designed Analog of an Antimicrobial Peptide, Crabrolin, Exhibits Enhanced Anti-Proliferative and In Vivo Antimicrobial Activity" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 19 (2023) . |
| APA | Yao, Aifang , Ma, Yingxue , Sun, Ruize , Zou, Wanchen , Chen, Xiaoling , Zhou, Mei et al. A Designed Analog of an Antimicrobial Peptide, Crabrolin, Exhibits Enhanced Anti-Proliferative and In Vivo Antimicrobial Activity . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (19) . |
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Abstract ：
Antimicrobial peptides (AMPs) that exert multiple functions are considered promising candidates to combat the bacterial drug resistance crisis. Nowadays, targeted peptide modification has been widely recognised to improve biological activity and make up for deficiencies in clinical applications such as toxicity. In this study, a helix-loop peptide was isolated and identified from the skin secretion of the Wuyi torrent frog Amolops wuyiensis, namely, ranatuerin-2-AW (R2AW) (GFMDTAKNVAKNVAATLLDKLKCKITGGC). Target modifications were made to R2AW to study the structure–activity relationships and to optimise its bioactivities. Five analogues were progressively designed via residue substitution and truncation and the antibacterial and anticancer activities were evaluated. We found that the serine-substitution and cyclic-domain-deletion products showed similar antibacterial activity to the natural peptide R2AW, implying that the disulphide bridge and Rana box were dispensable for the antibacterial activity of ranatuerin-2 peptides. Notably, the cationicity- and hydrophobicity-enhanced variant, [Lys4,19, Leu20]R2AW(1-22)-NH2, exhibited significantly optimised antibacterial and anticancer activities. Additionally, it killed bacteria by membrane disruption at a highly efficient rate. Moreover, [Lys4,19, Leu20]R2AW(1-22)-NH2 exerted potential in vivo efficacy in a methicillin-resistant Staphylococcus aureus (MRSA)-infected waxworm model. Overall, this study demonstrated some rational design ideas for optimising the dual antibacterial and anticancer activities of ranatuerin-2 peptides and it proposes [Lys4,19, Leu20]R2AW(1-22)-NH2 as an appealing candidate for therapeutic development.
Keyword ：
antimicrobial peptides (AMPs) antimicrobial peptides (AMPs) drug optimisation drug optimisation multidrug-resistant bacteria multidrug-resistant bacteria peptide design peptide design ranatuerin ranatuerin
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| GB/T 7714 | Aifang Yao , Tianxing Liu , Yuhai Cai et al. Progressive Design of a Ranatuerin-2 Peptide from Amolops wuyiensis: Enhancement of Bioactivity and In Vivo Efficacy [J]. | Antibiotics , 2023 , 13 (1) . |
| MLA | Aifang Yao et al. "Progressive Design of a Ranatuerin-2 Peptide from Amolops wuyiensis: Enhancement of Bioactivity and In Vivo Efficacy" . | Antibiotics 13 . 1 (2023) . |
| APA | Aifang Yao , Tianxing Liu , Yuhai Cai , Siqi Zhou , Xiaoling Chen , Mei Zhou et al. Progressive Design of a Ranatuerin-2 Peptide from Amolops wuyiensis: Enhancement of Bioactivity and In Vivo Efficacy . | Antibiotics , 2023 , 13 (1) . |
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