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学者姓名:唐点平
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Colorimetric immunoassays are widely used for biomarker detection, offering advantages of simplicity, sensitivity, and cost-effectiveness. Recent advancements focus on improving the catalytic activity of nanozymes for enhancing the sensitivity and accuracy of such assays. Bimetallic CuCo Prussian blue analog (CuCo PBA) has emerged as promising candidates due to their excellent peroxidase-like activity. However, their instant synthesis and integration into immunoassays for the rapid detection of biomarkers like carcinoembryonic antigen (CEA) remain underexplored. This study presents an innovative approach using CuCo PBA nanozymes for colorimetric immunoassays with immediate generation and application. In this study, CuCo PBA nanozymes were synthesized instantly by reacting Cu2+ with K-3[Co(CN)(6)] (<1 min), and their peroxidase-like activity was exploited for a colorimetric immunoassay system targeting CEA. The system demonstrated a clear blue color change upon the reaction of CuCo PBA with H2O2 and 3,3 ',5,5 '-tetramethylbenzidine (TMB), enabling sensitive detection. The assay was optimized for various parameters, including pH, temperature, and material ratio. A linear response was obtained for CEA detection over a concentration range of 0.05-60 ng/mL with a limit of detection (LOD) of 22 pg/mL. The integration of glucose oxidase (GOx) mediated the generation of H2O2, triggering the colorimetric reaction. This instantaneous CuCo PBA-based system effectively detected CEA in human serum samples, highlighting its potential for rapid diagnostic applications. This work introduces a novel approach for rapid and sensitive colorimetric immunoassays using CuCo PBA nanozymes that are synthesized on-demand and immediately applied. The system allows for efficient CEA detection with an exceptionally low detection limit, offering great potential for clinical diagnostics. The instant generation and application of CuCo PBA nanozymes in immunoassays represent a significant advancement in point-of-care testing technologies.
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GB/T 7714 | Li, Xiaoqin , Lin, Huizi , Chen, Xuwei et al. Instant synthesis of bimetallic CuCo PBA nanozyme for efficient colorimetric immunoassay of carcinoembryonic antigen [J]. | ANALYTICA CHIMICA ACTA , 2025 , 1354 . |
MLA | Li, Xiaoqin et al. "Instant synthesis of bimetallic CuCo PBA nanozyme for efficient colorimetric immunoassay of carcinoembryonic antigen" . | ANALYTICA CHIMICA ACTA 1354 (2025) . |
APA | Li, Xiaoqin , Lin, Huizi , Chen, Xuwei , Luo, Fenqiang , Zhang, Rong , Deng, Xiaoyan et al. Instant synthesis of bimetallic CuCo PBA nanozyme for efficient colorimetric immunoassay of carcinoembryonic antigen . | ANALYTICA CHIMICA ACTA , 2025 , 1354 . |
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The rise of antibiotic-resistant bacteria poses a serious global health threat, highlighting the urgent need for novel strategies beyond conventional antibiotic therapies. This study explores the potential of microbe-imprinted polymers (MIPs) as innovative, pathogen-specific affinity agents. Utilizing microbial surface-initiated polymerization, MIPs are in-situ synthesized on the surface of target microbes, creating flexible heteropolymers that precisely replicate microbial surface structures. This method exhibits high affinity (KD = 2.7x108 CFU/mL for E. coli) and selectivity at the strain level. MIPs offer significant advantages over traditional antibodies, including greater stability, cost-effectiveness, and a broader spectrum of binding capabilities, making them effective for identifying and targeting various microbial strains, including unidentified or drug-resistant variants. Moreover, their favorable biocompatibility and functional resilience in diverse environments position MIPs as promising candidates for rapid pathogen detection and therapeutic applications. This research paves the way for advanced biomimetic materials in microbe-specific diagnostics and combating infections, addressing the critical need for effective tools in antibiotic resistance surveillance.
Keyword :
Affinity Affinity Antibiotic resistance Antibiotic resistance Antibody mimics Antibody mimics Microbe-imprinted polymers Microbe-imprinted polymers Microbial recognition Microbial recognition
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GB/T 7714 | Wu, Yuanzi , Zhou, Kaiqiang , Li, Wenhui et al. Microbe-imprinted polymers for rapid drug-resistant bacteria recognition [J]. | CHEMICAL ENGINEERING JOURNAL , 2025 , 512 . |
MLA | Wu, Yuanzi et al. "Microbe-imprinted polymers for rapid drug-resistant bacteria recognition" . | CHEMICAL ENGINEERING JOURNAL 512 (2025) . |
APA | Wu, Yuanzi , Zhou, Kaiqiang , Li, Wenhui , Huan, Min , Yu, Zhichao , Yan, Fen et al. Microbe-imprinted polymers for rapid drug-resistant bacteria recognition . | CHEMICAL ENGINEERING JOURNAL , 2025 , 512 . |
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The yolk-shell structure has become a research hotspot in the field of photocatalysis in recent years due to its stability, movable yolk, and internal voids. However, yolk-shell has not been fully explored and applied in photoelectrochemical (PEC) immunoassays. In this work, a smartphone-based portable PEC immunoassay was constructed by using high-entropy yolk-shell Au@(ZnCdMnGaCu)(x)S as a photosensitive material, showing satisfactory detection with the assistance of localized surface plasmon resonance (LSPR). Specifically, a typical sandwich reaction introduces a detection antibody modified and labeled with gold nanoparticles (Au NPs) of alkaline phosphatase (ALP) into the system with the presence of prostate-specific antigen (PSA). Ascorbic acid (AA) content increased with increasing PSA and showed a linear enhancement of photocurrent at PSA concentrations of 0.01-50 ng mL(-1) with a limit of detection of 8.94 pg mL(-1). Additionally, the proposed assay technology not only provides a portable detection system for the immediate detection of tumor markers, but also offers a promising paradigm for the development of high-entropy materials.
Keyword :
High-entropy yolk-shell Au@(ZnCdMnGaCu)(x)S High-entropy yolk-shell Au@(ZnCdMnGaCu)(x)S Localized surface plasmon resonance Localized surface plasmon resonance Photoelectrochemical immunoassay Photoelectrochemical immunoassay Portable inspection system Portable inspection system Prostate-specific antigen Prostate-specific antigen Smartphone Smartphone
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GB/T 7714 | Wan, Xinyu , Wang, Xin , Wang, Haiyang et al. Localized surface plasmon resonance-enhanced photoelectrochemical immunoassay based on high-entropy yolk-shell Au@(ZnCdMnGaCu)xS [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 422 . |
MLA | Wan, Xinyu et al. "Localized surface plasmon resonance-enhanced photoelectrochemical immunoassay based on high-entropy yolk-shell Au@(ZnCdMnGaCu)xS" . | SENSORS AND ACTUATORS B-CHEMICAL 422 (2025) . |
APA | Wan, Xinyu , Wang, Xin , Wang, Haiyang , Wei, Qiaohua , Tang, Dianping . Localized surface plasmon resonance-enhanced photoelectrochemical immunoassay based on high-entropy yolk-shell Au@(ZnCdMnGaCu)xS . | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 422 . |
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Nanozyme-based electrochemical biosensors have emerged as an alternative to enzyme-based biosensors for next-generation bioanalysis. However, potential antibody modifications limit the catalytic sites of the nanozyme, thereby reducing sensor sensitivity. Here, a sensitive method for determining carcinoembryonic antigen (CEA) was developed. It involved coupling a cascade enzyme-enzyme-like catalytic reaction using Fe-Co Prussian blue analog nanozymes with high peroxidase-like activity (79.42 U mg-1). Briefly, the transduction of biological signals to chemical signals was achieved through the strategy centered on catalytic electroactive probes. Thereafter, with the assistance of the microelectrochemical workstation, the output of signals was realized. The platform exhibited an ultra-wide range of 0.020-100 ng mL-1 and a detection limit of 0.013 ng mL-1 CEA, which was mainly attributed to the excellent peroxidase activity, good conductivity, and synergistic amplification of current signals of synthesized nanozymes. In addition, the modification-free features greatly reduced the complexity of the bioassay and significantly improves its portability and cost-effectiveness. Overall, this study advances the development of nanozymes and their electrochemical biosensing applications and is expected to extend to the development of miniaturized devices in direct detection environments.
Keyword :
Bifunctional nanozyme Bifunctional nanozyme Carcinoembryonic antigen Carcinoembryonic antigen Catalytic amplification Catalytic amplification Electrochemical immunoassay Electrochemical immunoassay
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GB/T 7714 | Chen, Shuyun , Tian, Shuo , Wang, Yunsen et al. Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay [J]. | BIOSENSORS & BIOELECTRONICS , 2025 , 278 . |
MLA | Chen, Shuyun et al. "Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay" . | BIOSENSORS & BIOELECTRONICS 278 (2025) . |
APA | Chen, Shuyun , Tian, Shuo , Wang, Yunsen , Li, Meijin , Tang, Dianping . Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay . | BIOSENSORS & BIOELECTRONICS , 2025 , 278 . |
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The judicious utilization of antibiotics has established a robust bulwark for human health. However, their improper usage has engendered deleterious ramifications on the environment, underscoring the imperative for developing efficacious and cost-effective detection and degradation platforms. This study presents a sulfur-modified iron-cobalt bimetallic single-atom nitrogen-doped carbon catalyst (S-FeCo-NC) with a noncopper active center. In contrast to conventional laccase, which utilizes copper as its active center, the S-FeCo-NC catalyst exhibits multiple enzyme activities, including laccase-like, peroxidase-like, and catalase-like functions, with iron and cobalt serving as the active centers. As a proof of concept, the combined laccase-like and catalase-like functions of S-FeCo-NC were used as independent signal outputs, while a multienzyme cascade dual-mode assay system was designed for the rapid detection of tetracycline (TC) in combination with peroxidase-like enzymes. In this system, oxygen directly participated in the catalytic process of laccase-like as an electron acceptor, while catalase-like peroxidase efficiently catalyzed the production of O2 from H2O2. The elevated concentration of O2 offered a unique advantage for the increased catalytic activity of the laccase-like enzyme, which outputs visually resolved colorimetric signals using stable 4-aminopyridine with oxidized TC. Furthermore, the peroxidase-like activity of S-FeCo-NC catalyzed the generation of OH radicals with strong oxidative properties, and these radicals carried out effective oxidative decomposition of TC. The signal output of the response of the catalytic process was performed using differential pulse cyclic voltammetry, which further improved the sensitivity and accuracy of the detection. The experimental findings demonstrate that the detection system exhibits a favorable response signal to TC within the range of 0.005-500 mu M, with its detection range reaching 0.5-500 and 0.005-1.00 mu M, respectively, and the detection limit is as low as 0.22 mu M and 1.68 nM, respectively. This cascade dual-mode detection system, based on multienzyme activity, has been shown to significantly enhance the catalytic activity of laccase, while also demonstrating stability in a lower detection range. This suggests that it may offer a novel approach for the sensitive detection and degradation of environmental pollutants.
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GB/T 7714 | Wang, Yunsen , Tian, Shuo , Chen, Shuyun et al. S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (13) : 7526-7535 . |
MLA | Wang, Yunsen et al. "S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay" . | ANALYTICAL CHEMISTRY 97 . 13 (2025) : 7526-7535 . |
APA | Wang, Yunsen , Tian, Shuo , Chen, Shuyun , Li, Meijin , Tang, Dianping . S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay . | ANALYTICAL CHEMISTRY , 2025 , 97 (13) , 7526-7535 . |
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Accurate and rapid identification of expired and spoiled food is crucial for food conservation, reducing resource wastage, and preventing food poisoning. This paper presents a portable electrochemical sensing platform supported by a miniature electrochemical workstation and an intelligent pH recognition system, enabling detection of L-lactic acid (L-LA) in food products without enzyme involvement. The system was based on a Cu2O@CuO multifaceted extended spatial hexapod structure. The synthesized Cu2O@CuO was characterized by a welldefined multifaceted structure. Significant enzyme-free catalysis was exhibited, and pH responses dominated by anthocyanins were identified through an intelligent image acquisition system. Additionally, we developed an electrochemical detection device for pH assistance during target testing, addressing the limitations of current electrochemical sensors' complex signal acquisition components using 3D-printed fabrication techniques and smartphones. The proposed multifunctional electrochemical workbench based on Cu2O@CuO was found to offer a preferable linear detection range of 0.1-1000 mu M for L-LA, with a low detection limit of 0.027 mu M. The visualization of pH determination was introduced as a novel approach for developing advanced electrochemical workbenches. In conclusion, pH-assisted portable electrochemical detection systems hold great potential for immediate food safety identification, particularly in resource-limited areas, facilitating prompt diagnosis and ensuring food safety.
Keyword :
3D-printed device 3D-printed device Electrochemical detection Electrochemical detection Enzyme-free catalysis Enzyme-free catalysis Food quality evaluation Food quality evaluation Visualization Visualization
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GB/T 7714 | Wu, Di , Wei, Qiaohua , Yu, Zhichao et al. Miniature dual-channel electrochemical 3D-printed sensing platform for enzyme-free screening of L-lactic acid in foodstuffs accompanying pH recognition [J]. | FOOD CHEMISTRY , 2025 , 465 . |
MLA | Wu, Di et al. "Miniature dual-channel electrochemical 3D-printed sensing platform for enzyme-free screening of L-lactic acid in foodstuffs accompanying pH recognition" . | FOOD CHEMISTRY 465 (2025) . |
APA | Wu, Di , Wei, Qiaohua , Yu, Zhichao , Gao, Yuan , Knopp, Dietmar , Tang, Dianping . Miniature dual-channel electrochemical 3D-printed sensing platform for enzyme-free screening of L-lactic acid in foodstuffs accompanying pH recognition . | FOOD CHEMISTRY , 2025 , 465 . |
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Photocurrent-polarity conversion strategies are typically realized by constructing complex photovoltaic electrodes or changing the relevant conditions, but most involve poor photogenerated carrier transfer efficiency and cumbersome experimental steps. To this end, a photoelectrochemical (PEC) biosensor by utilizing ascorbic acid (AA)-induced photocurrent-polarity-switching was proposed for the detection of carcinoembryonic antigen (CEA). Under light excitation, the electron donor AA was oxidized by the photogenerated holes of photoactive material Co-NC/CdS, resulting in the conversion of cathodic photocurrent to the anodic direction. In the presence of the target CEA, alkaline phosphatase (ALP) was introduced into the microplates by the sandwiched immunoreaction, which then catalyzed the production of AA from ascorbic acid-2-phosphate (AAP). Finally, the catalytic product AA was transferred onto Co-NC/CdS-modified screen-printed carbon electrode, thus activating photocurrent-polarity-switching platform. The anodic photocurrent values gradually increased with increasing CEA concentration in the range of 0.02-80 ng mL(-1) and reached a limit of detection (LOD) of 8.47 pg mL(-1) (S/N = 3). In addition, the results of actual sample detection prove the reliability of the constructed PEC biosensor. Importantly, this work relies on a mobile smartphone wireless Bluetooth device coupled with the PEC biosensor for immediate detection, providing another idea for detecting CEA in clinical diagnosis.
Keyword :
Carcinoembryonic antigen Carcinoembryonic antigen Photocurrent-polarity-switching Photocurrent-polarity-switching Photoelectrochemical immunoassay Photoelectrochemical immunoassay Point-of-care Point-of-care Wireless bluetooth Wireless bluetooth
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GB/T 7714 | Wang, Xin , Wang, Haiyang , Wan, Xinyu et al. Smartphone-based point-of-care photoelectrochemical immunoassay coupling with ascorbic acid-triggered photocurrent-polarity conversion switching [J]. | BIOSENSORS & BIOELECTRONICS , 2025 , 267 . |
MLA | Wang, Xin et al. "Smartphone-based point-of-care photoelectrochemical immunoassay coupling with ascorbic acid-triggered photocurrent-polarity conversion switching" . | BIOSENSORS & BIOELECTRONICS 267 (2025) . |
APA | Wang, Xin , Wang, Haiyang , Wan, Xinyu , Wei, Qiaohua , Zeng, Yongyi , Tang, Dianping . Smartphone-based point-of-care photoelectrochemical immunoassay coupling with ascorbic acid-triggered photocurrent-polarity conversion switching . | BIOSENSORS & BIOELECTRONICS , 2025 , 267 . |
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The quantification of intracellular hydrogen peroxide (H2O2) serves as a critical biomarker for characterizing cellular physiological states, providing essential insights into metabolic regulation and signaling pathways. This analytical paradigm not only advances our understanding of pathological mechanisms but also contributes to the development of novel diagnostic approaches and precision therapeutic interventions. Herein, we established an innovative electrochemical microsensing platform capable of discriminating between malignant and normal cells through their distinct inflammatory responses under external stimulation. This innovative methodology integrates three critical technical advancements: (i) optimization of a one-pot microwave synthesis protocol for fabricating high-performance PtZnCd nanoparticles anchored on multi-walled carbon nanotubes (MWCNTs), which serve as the core sensing element; (ii) systematic electrochemical characterization coupled with density functional theory (DFT) calculations demonstrating that this hybrid architecture significantly reduces interfacial charge-transfer resistance while enhancing heterogeneous electron transfer kinetics; (iii) comprehensive biocompatibility evaluations confirming the composite material's favorable cytotoxicity profile and biological safety, supporting its potential for cellular classification applications. Through real-time monitoring of dynamic metabolic fluctuations and intracellular inflammatory microenvironment changes in response to ascorbic acid (AA) and dehydroascorbic acid (DHA) stimulation, we established distinct response signatures that effectively differentiate neoplastic cells from their healthy counterparts. This study introduces an innovative electrochemical sensing paradigm that synergistically combines biocompatible nanocatalysts with inflammatory microenvironment dynamics, establishing a robust platform for dual discrimination between cancerous and normal cells, with significant implications for biomedical research and clinical diagnostics. © 2025 Elsevier B.V.
Keyword :
Multiwalled carbon nanotubes (MWCN) Multiwalled carbon nanotubes (MWCN)
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GB/T 7714 | Xu, Man , Yu, Zhichao , Wei, Qiaohua et al. Inflammatory microenvironment-responsive electrochemical biosensing for cancer cell discrimination using PtZnCd-anchored multi-walled carbon nanotubes [J]. | Talanta , 2025 , 294 . |
MLA | Xu, Man et al. "Inflammatory microenvironment-responsive electrochemical biosensing for cancer cell discrimination using PtZnCd-anchored multi-walled carbon nanotubes" . | Talanta 294 (2025) . |
APA | Xu, Man , Yu, Zhichao , Wei, Qiaohua , Tang, Dianping . Inflammatory microenvironment-responsive electrochemical biosensing for cancer cell discrimination using PtZnCd-anchored multi-walled carbon nanotubes . | Talanta , 2025 , 294 . |
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Sluggish charge transfer and rapid electron-hole recombination severely limit the analytical performance of photoelectrochemical (PEC) immunoassays. This work presented a PEC immunosensing strategy that employed a target-induced enzyme-catalyzed reaction to in-situ generate oxygen vacancy (Ov) for amplifying the photocurrent detection of carcinoembryonic antigen (CEA). Concretely, ascorbic acid-2-phosphate (AAP) was catalyzed to produce ascorbic acid (AA) by alkaline phosphatase (ALP) in the presence of CEA. The generated AA could serve as a reducing agent to introduce oxygen vacancy (Ov) into the etching tungsten trioxide (E-WO3) photoanode, resulting in an Ov-enriched E-WO3 (E-WO3-Ov) photoanode. The formation of Ov allowed efficient introduction of defect levels into the energy band structure of E-WO3-Ov photoanode, resulting in high charge transfer and electron-hole separation efficiency for photocurrent amplification. Later, it was applied to fabricate a PEC immunosensor, thus enabling a wide linear range from 0.02 to 80 ng/mL and a low detection limit of 12.9 pg/mL. Overall, this work presented a promising sensing strategy for PEC immunosensors, expanding the scope of potential applications in bioassays and clinical diagnostics.
Keyword :
Charge transfer Charge transfer Defect level Defect level Electron-hole separation Electron-hole separation Oxygen vacancy Oxygen vacancy Photoelectrochemical immunoassay Photoelectrochemical immunoassay
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GB/T 7714 | Qin, Jiao , Yu, Zhichao , Wu, Di et al. Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay [J]. | BIOSENSORS & BIOELECTRONICS , 2025 , 279 . |
MLA | Qin, Jiao et al. "Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay" . | BIOSENSORS & BIOELECTRONICS 279 (2025) . |
APA | Qin, Jiao , Yu, Zhichao , Wu, Di , Li, Meijin , Tang, Dianping . Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay . | BIOSENSORS & BIOELECTRONICS , 2025 , 279 . |
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Early detection of cancer has a profound impact on patient survival and treatment outcomes considering high treatment success rates and reduced treatment complexity. Here, we developed a portable photoelectrochemical (PEC) immune platform for sensitive testing of alpha-fetoprotein (AFP) based on Pt nanocluster (Pt NCs) loaded defective -state g-C3N4 photon -electron transducers. The broad forbidden band structure of g-C3N4 was optimized by the nitrogen doping strategy and additional homogeneous porous structure was introduced to further enhance the photon utilization. In addition, the in -situ growth of Pt NCs provided efficient electron transfer catalytic sites for sacrificial agents, which were used to further improve the sensitivity of the sensor. Efficient photoelectric conversion under a hand-held flashlight was determined by the geometry of the transducer and the energy band design, and the portable design of the PEC sensor was realized. The developed sensing platform exhibited a wide linear response range (0.1-50 ng mL-1) and low limit of detection (0.043 ng mL-1) for AFP under optimum conditions. This work provides a new idea for designing portable PEC biosensing platforms to meet the current mainstream POC testing needs.
Keyword :
Alpha-fetoprotein Alpha-fetoprotein Micro-electro-mechanical-system Micro-electro-mechanical-system Photoelectrochemical immunoassay Photoelectrochemical immunoassay Platinum nanoclusters Platinum nanoclusters
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GB/T 7714 | Lou, Fangming , Wang, Shaojie , Han, Bo et al. Portable photoelectrochemical immunoassay with micro-electro-mechanical-system for alpha-fetoprotein in hepatocellular carcinoma [J]. | ANALYTICA CHIMICA ACTA , 2024 , 1298 . |
MLA | Lou, Fangming et al. "Portable photoelectrochemical immunoassay with micro-electro-mechanical-system for alpha-fetoprotein in hepatocellular carcinoma" . | ANALYTICA CHIMICA ACTA 1298 (2024) . |
APA | Lou, Fangming , Wang, Shaojie , Han, Bo , Li, Qunfang , Tang, Dianping . Portable photoelectrochemical immunoassay with micro-electro-mechanical-system for alpha-fetoprotein in hepatocellular carcinoma . | ANALYTICA CHIMICA ACTA , 2024 , 1298 . |
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