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学者姓名：洪晶
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Abstract ：
为探索烟草废弃物多用途应用的潜力及提取方法对多糖结构和功能的影响,通过直接提取和酶制剂辅助提取工艺对烟草废弃物中的多糖进行提取,采用响应面法优化了酶制剂辅助提取的工艺条件,并分析了多糖的结构和抗氧化活性.结果表明:①在料液比为1∶32、每克烟草废弃物中纤维素酶添加量为0.455 g(酶活为5 000 U)、酶解温度为62 ℃及酶解时间为2.0 h条件下,多糖得率最高,为23.56％.②直接提取和纤维素酶辅助提取的多糖主要为含有β-构型吡喃环的酸性多糖,且单糖组成相似,纤维素酶辅助提取的多糖呈光滑小颗粒状,聚合度和交联度较低.③直接提取和纤维素酶辅助提取的多糖对DPPH和ABTS自由基均具有一定的清除能力,表现出一定的抗氧化活性.该研究为烟草废弃物中多糖的提取、结构表征及其生物活性分析提供了理论支撑,也为烟草废弃物的高值化利用开辟了新途径.
Keyword ：
多糖 多糖 工艺优化 工艺优化 烟草废弃物 烟草废弃物 结构表征 结构表征 酶制剂 酶制剂
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| GB/T 7714 | 操晓亮 , 张泽晓 , 马鹏飞 et al. 烟草废弃物中多糖的提取及结构功能分析 [J]. | 烟草科技 , 2025 , 58 (4) : 63-72 . |
| MLA | 操晓亮 et al. "烟草废弃物中多糖的提取及结构功能分析" . | 烟草科技 58 . 4 (2025) : 63-72 . |
| APA | 操晓亮 , 张泽晓 , 马鹏飞 , 罗金妍 , 张峰 , 李斌 et al. 烟草废弃物中多糖的提取及结构功能分析 . | 烟草科技 , 2025 , 58 (4) , 63-72 . |
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Abstract ：
Wuyi rock tea residue is rich in protein, making them a valuable protein source for producing angiotensin Iconverting enzyme (ACE) inhibitory peptides. In the present research, five novel ACE inhibitory peptides were isolated and identified from protein hydrolysate of Wuyi rock tea residue using Sephadex G-15, reversed phase high-performance liquid chromatography (RP-HPLC), and quadrupole time-of-flight mass spectrometry. The peptide sequences were identified as FPFPRPP, PPPRGP, PFPRPPH, LGHPW and LKFPDF, with IC50 values ranged from 0.271 mg/mL to 1.018 mg/mL, with FPFPRPP demonstrating the strongest activity. As indicated by the Lineweaver-Burk plot, FPFPRPP was a mixed-type inhibitor against ACE. Molecular docking studies further confirmed that FPFPRPP forms five hydrogen bonds with residues near the active sites of ACE, with a binding energy of -10.5 kcal/mol. Functional assay in human umbilical vein endothelial cells (HUVECs) showed that FPFPRPP significantly upregulated nitric oxide production and downregulated reactive oxygen species and endothelin-1 levels induced by angiotensin II, indicating its potential antihypertensive effect. These findings highlight Wuyi rock tea residue as a promising source of ACE inhibitory peptides and suggest FPFPRPP could be developed as a functional food ingredient with possible applications in hypertension management.
Keyword ：
Angiotensin I-Converting enzyme Angiotensin I-Converting enzyme Molecular mechanism Molecular mechanism Peptides Peptides Residue Residue Wuyi rock tea Wuyi rock tea
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| GB/T 7714 | Cao, Xiaoliang , Zhou, Huiyuan , Xie, Jindong et al. A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from Wuyi rock tea residue: Preparation, identification, and its potential molecular mechanism [J]. | LWT-FOOD SCIENCE AND TECHNOLOGY , 2025 , 216 . |
| MLA | Cao, Xiaoliang et al. "A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from Wuyi rock tea residue: Preparation, identification, and its potential molecular mechanism" . | LWT-FOOD SCIENCE AND TECHNOLOGY 216 (2025) . |
| APA | Cao, Xiaoliang , Zhou, Huiyuan , Xie, Jindong , Zhang, Zexiao , Guo, Songbin , Luo, Jinyan et al. A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from Wuyi rock tea residue: Preparation, identification, and its potential molecular mechanism . | LWT-FOOD SCIENCE AND TECHNOLOGY , 2025 , 216 . |
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Abstract ：
Wuyi rock tea residues, generated after brewing or aroma extraction, are rich in nutrients. This study aimed to prepare ACE inhibitory peptides from the protein hydrolysate of Wuyi rock tea residues and investigate their stability. The optimal proteolysis conditions for neutral protease were optimized as: a substrate concentration of 2% (w/v), pH 7.0, hydrolysis temperature of 45 degrees C, enzymatic digestion time of 3 h, and enzyme dosage of 6800 U/g. Under these conditions, the ACE inhibition rate of protein hydrolysate of Wuyi rock tea residues reached 70.47 +/- 0.29% at a concentration of 0.5 mg/mL. Additionally, the prepared ACE inhibitory peptides demonstrated excellent stability against acid, base, heat, and certain metal ions. These findings suggest that the proteins extracted from Wuyi rock tea residues can serve as a promising source to produce highly active ACE inhibitory peptides. These peptides could be utilized in the development of nutraceuticals, functional foods, and pharmaceuticals.
Keyword ：
ACE inhibitory peptides ACE inhibitory peptides response surface methodology (RSM) response surface methodology (RSM) stability stability Wuyi rock tea residues Wuyi rock tea residues
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| GB/T 7714 | Cao, Xiaoliang , Zhou, Huiyuan , Xie, Jindong et al. Preparation and stability of angiotensin-I-converting enzyme (ACE) inhibitory peptides from protein hydrolysate of Wuyi rock tea residues [J]. | CYTA-JOURNAL OF FOOD , 2024 , 22 (1) . |
| MLA | Cao, Xiaoliang et al. "Preparation and stability of angiotensin-I-converting enzyme (ACE) inhibitory peptides from protein hydrolysate of Wuyi rock tea residues" . | CYTA-JOURNAL OF FOOD 22 . 1 (2024) . |
| APA | Cao, Xiaoliang , Zhou, Huiyuan , Xie, Jindong , Huang, Linxia , Guo, Songbin , Zhang, Zexiao et al. Preparation and stability of angiotensin-I-converting enzyme (ACE) inhibitory peptides from protein hydrolysate of Wuyi rock tea residues . | CYTA-JOURNAL OF FOOD , 2024 , 22 (1) . |
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为研究外源酶对陈年武夷岩茶挥发性成分的影响,运用气相色谱-质谱联用技术(gas chromatography-mass spectrometry,GC-MS)、感官评价和香气活性值(odor activity value,OAV)方法对酶处理后的武夷岩茶进行分析.GC-MS分析结果表明,纤维素酶、果胶酶、漆酶和β-葡萄糖苷酶质量比为2:1:2:3时有利于香气物质的释放,尤其对醇类、酯类及酮类香气物质释放贡献较大.感官评价和OAV值的结果表明,酶的添加能明显提高武夷岩茶的花香、甜香和木香,复合酶的处理效果最佳,其次是β-葡萄糖苷酶.经复合酶处理后己醛、2-己烯醛和(E,E)-2,4-己二烯醛等物质含量降低,OAV减小.上述研究表明,纤维素酶、果胶酶、漆酶、β-葡萄糖苷酶及其复合酶的添加均可明显改善陈年武夷岩茶香气品质.
Keyword ：
β-葡萄糖苷酶 β-葡萄糖苷酶 果胶酶 果胶酶 武夷岩茶 武夷岩茶 气相色谱-质谱法 气相色谱-质谱法 漆酶 漆酶 纤维素酶 纤维素酶 香气活性值 香气活性值
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| GB/T 7714 | 谢李玲 , 薛婉茹 , 李丹阳 et al. 外源酶对陈年武夷岩茶香气品质的改善作用 [J]. | 食品研究与开发 , 2023 , 44 (5) : 155-164 . |
| MLA | 谢李玲 et al. "外源酶对陈年武夷岩茶香气品质的改善作用" . | 食品研究与开发 44 . 5 (2023) : 155-164 . |
| APA | 谢李玲 , 薛婉茹 , 李丹阳 , 周惠媛 , 孟春 , 洪晶 . 外源酶对陈年武夷岩茶香气品质的改善作用 . | 食品研究与开发 , 2023 , 44 (5) , 155-164 . |
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In this work, four novel angiotensin I-converting enzyme inhibitory (ACEI) peptides were isolated and identified from peony (Paeonia suffruticosa Andr.) seed hydrolysate (PSH) by consecutive chromatography and UPLC-ESIQTOF-MS/MS. The IC50 values of HWS (0.640 +/- 0.016 mg/mL) and VLSGF (0.328 +/- 0.040 mg/mL) were significantly (p < 0.05) lower than those of the other two peptides. The Lineweaver-Burk plots showed that VLSGF is competitive inhibition against ACE and HWS acted as a mixed-type inhibitor. Meanwhile, the results of molecular docking showed hydrogen interactions between VLSGF/HWS and ACE. In human endothelial cells, the pretreatments of VLSGF and HWS could increase the production of nitric oxide (NO) and the expression of endothelial nitric oxide synthase (eNOS). In addition, they also decreased the reactive oxygen species (ROS) production. These results indicated that it may be possible to use peony seed protein hydrolysate to create ingredients for functional foods.
Keyword ：
ACEI peptides ACEI peptides Human umbilical vein endothelial cells Human umbilical vein endothelial cells inhibitory mechanism inhibitory mechanism molecular docking molecular docking purification purification
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| GB/T 7714 | Ye, Shufang , Chen, Qiuluan , Li, Danyang et al. Isolation and identification of novel angiotensin I-converting enzyme (ACE) inhibitory peptides from Pony Seed and evaluation of the inhibitory mechanisms [J]. | JOURNAL OF FUNCTIONAL FOODS , 2022 , 95 . |
| MLA | Ye, Shufang et al. "Isolation and identification of novel angiotensin I-converting enzyme (ACE) inhibitory peptides from Pony Seed and evaluation of the inhibitory mechanisms" . | JOURNAL OF FUNCTIONAL FOODS 95 (2022) . |
| APA | Ye, Shufang , Chen, Qiuluan , Li, Danyang , Zhou, Huiyuan , Chen, Yanbin , Meng, Chun et al. Isolation and identification of novel angiotensin I-converting enzyme (ACE) inhibitory peptides from Pony Seed and evaluation of the inhibitory mechanisms . | JOURNAL OF FUNCTIONAL FOODS , 2022 , 95 . |
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In this study, we attempted to prepare novel angiotensin I-converting enzyme inhibitory (ACEI) peptides from Tie Guanyin tea residue protein (TTRP). Different proteases were used to hydrolyze TTRP to prepare ACEI peptides, neutral protease and alkaline protease hydrolysates exhibited higher ACEI activity and was chosen in further hydrolysis treatment. Response surface methodology (RSM) was used to optimize the enzymatic condition, and the results indicated that the optimal conditions of enzymatic hydrolysis were: 4% (w/v) of TTRP in water was hydrolyzed with 3500 U/g neutral protease at 50 degrees C and pH 6.5 for 2.5 h in the first step and then with 5000 U/g alkaline protease at 50 degrees C and pH 10.0 for 3 h in the second step. Under the optimum condition, the ACE inhibitory rate of the final products was 88.45% +/- 0.45% at the concentration of 1.0 mg/mL. Moreover, ACEI peptides derived from TTRP showed good stability under different temperatures, pHs, metal ions and gastrointestinal digestion. These results indicated that the TTRP has potential to be used for preparing ACEI peptides.
Keyword ：
ACEI peptides ACEI peptides response surface methodology (RSM) response surface methodology (RSM) stability stability Tie Guanyin tea residue protein Tie Guanyin tea residue protein two-step enzymatic hydrolysis two-step enzymatic hydrolysis
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| GB/T 7714 | Ye, Shufang , Luo, Jinyan , Lin, Jiarong et al. Preparation of angiotensin I-converting enzyme (ACE) inhibitory peptides from Tie Guanyin tea residue protein using two-step enzymatic hydrolysis [J]. | FOOD SCIENCE AND TECHNOLOGY , 2022 , 42 . |
| MLA | Ye, Shufang et al. "Preparation of angiotensin I-converting enzyme (ACE) inhibitory peptides from Tie Guanyin tea residue protein using two-step enzymatic hydrolysis" . | FOOD SCIENCE AND TECHNOLOGY 42 (2022) . |
| APA | Ye, Shufang , Luo, Jinyan , Lin, Jiarong , Meng, Chun , Hong, Jing . Preparation of angiotensin I-converting enzyme (ACE) inhibitory peptides from Tie Guanyin tea residue protein using two-step enzymatic hydrolysis . | FOOD SCIENCE AND TECHNOLOGY , 2022 , 42 . |
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本发明公开了一种来源于紫苏籽粕的ACE抑制肽及其制备方法与应用。所述ACE抑制肽的氨基酸序列为Val‑Gly‑Ala‑Tyr。通过体外ACE抑制活性测定发现，该紫苏籽粕ACE抑制肽的IC50为0.861 mg/mL，其浓度为0.05 mg/mL时，能有效提高人脐静脉内皮细胞NO的释放量，并且对人脐静脉内皮细胞无毒副作用。本发明提供的紫苏籽粕ACE抑制肽具有结构简单、安全、活性强的特点，且易于进行生产，有望为制备高血压预防药物提供有效成分，或作为功能食品添加剂供高血压患者食用。
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| GB/T 7714 | 洪晶 , 陈雪芹 , 叶淑芳 et al. 一种来源于紫苏籽粕的ACE抑制肽及其制备方法与应用 : CN202110862237.0[P]. | 2021-07-29 . |
| MLA | 洪晶 et al. "一种来源于紫苏籽粕的ACE抑制肽及其制备方法与应用" : CN202110862237.0. | 2021-07-29 . |
| APA | 洪晶 , 陈雪芹 , 叶淑芳 , 陈彦彬 . 一种来源于紫苏籽粕的ACE抑制肽及其制备方法与应用 : CN202110862237.0. | 2021-07-29 . |
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以牡丹籽粕为原料,用酶解法制备ACE抑制肽及其稳定性研究.以血管紧张素转化酶(angiotension converting enzyme,ACE)抑制率为指标,从中性蛋白酶、碱性蛋白酶、胃蛋白酶、胰蛋白酶和风味蛋白酶中筛选出最佳ACE抑制肽制备酶为中性蛋白酶.以单因素实验为基础,进行酶解条件的响应面优化,结果显示牡丹籽ACE抑制肽酶法制备的最优条件为:底物浓度2％,pH7.5,加酶量7200 U/g,酶解温度43℃,酶解时间2h,此时酶解液ACE抑制率可达到86.93％±2.38％.此外,稳定性分析显示该ACE抑制肽具有良好的温度和酸碱稳定性,在温度20 ～ 100℃与pH2～10的环境下,ACE抑制活性没有显著变化(P＞0.05),并且经过体外胃肠模拟消化后,ACE抑制活性变化不显著(P＞0.05),仍能保持良好的抑制活性.
Keyword ：
ACE抑制肽 ACE抑制肽 中性蛋白酶 中性蛋白酶 响应面 响应面 牡丹籽粕 牡丹籽粕 稳定性 稳定性
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| GB/T 7714 | 陈秋銮 , 陈雪芹 , 马倩 et al. 酶解法制备牡丹籽ACE抑制肽及其稳定性 [J]. | 食品工业科技 , 2020 , 41 (19) : 149-156 . |
| MLA | 陈秋銮 et al. "酶解法制备牡丹籽ACE抑制肽及其稳定性" . | 食品工业科技 41 . 19 (2020) : 149-156 . |
| APA | 陈秋銮 , 陈雪芹 , 马倩 , 谢李玲 , 薛婉茹 , 孟春 et al. 酶解法制备牡丹籽ACE抑制肽及其稳定性 . | 食品工业科技 , 2020 , 41 (19) , 149-156 . |
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Mycobacterium tuberculosis (Mtb) is the key devastating bacterial pathogen responsible for tuberculosis. Increasing emergence of multi-drug-resistant, extensively drug-resistant, and rifampicin/isoniazid-resistant strains of Mtb makes the discovery of validated drug targets an urgent priority. As a vital translational component of the protein biosynthesis system, elongation factor Tu (EF-Tu) is an important molecular switch responsible for selection and binding of the cognate aminoacyl-tRNA to the acceptor site on the ribosome. In addition, EF-Tu from Mtb (MtbEF-Tu) is involved in the initial step of trans-translation which is an effective system for rescuing the stalled ribosomes from non-stop translation complexes under stress conditions. Given its crucial role in protein biosynthesis, EF-Tu is identified as an excellent molecular target for drug design. Here, we reported the recombinant expression, purification, biophysical characterization, and structural modeling of the MtbEF-Tu protein. Our results demonstrated that prokaryotic expression plasmids of pET28a-MtbEF-Tu could be expressed efficiently in Escherichia coli. We successfully purified the 6x His-tagged proteins with a yield of 16.8 mg from 1 l of Luria Bertani medium. Dynamic light scattering experiments showed that MtbEF-Tu existed in a monomeric form, and circular dichroism experiments indicated that MtbEF-Tu was well structured. Moreover, isothermal titration calorimetry experiments displayed that the purified MtbEF-Tu protein possessed intermediate binding affinities for guanosine-5-triphosphate (GTP) and GDP. The GTP/GDP-binding sites were predicted by flexible molecular docking approach which reveals that GTP/GDP binds to MtbEF-Tu mainly through hydrogen bonds. Our work lays the essential basis for further structural and functional studies of MtbEF-Tu as well as MtbEF-Tu-related novel drug developments.
Keyword ：
MtbEF-Tu MtbEF-Tu protein biosynthesis protein biosynthesis protein expression and purification protein expression and purification protein-guanine nucleotide interaction protein-guanine nucleotide interaction tuberculosis tuberculosis
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| GB/T 7714 | Yang, Juanjuan , Hong, Jing , Luo, Ling et al. Biophysical characterization and ligand-binding properties of the elongation factor Tu from Mycobacterium tuberculosis [J]. | ACTA BIOCHIMICA ET BIOPHYSICA SINICA , 2019 , 51 (2) : 139-149 . |
| MLA | Yang, Juanjuan et al. "Biophysical characterization and ligand-binding properties of the elongation factor Tu from Mycobacterium tuberculosis" . | ACTA BIOCHIMICA ET BIOPHYSICA SINICA 51 . 2 (2019) : 139-149 . |
| APA | Yang, Juanjuan , Hong, Jing , Luo, Ling , Liu, Ke , Meng, Chun , Ji, Zhi-liang et al. Biophysical characterization and ligand-binding properties of the elongation factor Tu from Mycobacterium tuberculosis . | ACTA BIOCHIMICA ET BIOPHYSICA SINICA , 2019 , 51 (2) , 139-149 . |
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为从胡萝卜籽中纯化具有较高活性的抗氧化肽.采用凝胶过滤色谱和反向高效液相色谱法对胡萝卜籽蛋白酶解液进行分离纯化,以DPPH自由基清除率为指标,最终获得2个活性较强的抗氧化肽组分F3-a和F3-b.通过液质联用LC-ESI-MS/M分析得到其氨基酸序列分别为Lys-Asp-Asn-Phe-Leu-Phe(KDNFLF)和Leu-Phe(LF).此外,对2个多肽的ABTS自由基清除率、超氧阴离子自由基清除率和脂质过氧化抑制能力进行了测定,KDNFLF和LF均表现出较强的自由基清除活性.说明纯化的胡萝卜籽抗氧化肽具有良好的活性,可以作为潜在的抗氧化剂应用于食品工业及医药行业中.
Keyword ：
分离纯化 分离纯化 抗氧化肽 抗氧化肽 胡萝卜籽 胡萝卜籽
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| GB/T 7714 | 王惠敏 , 户佩 , 蔡甜甜 et al. 抗氧化胡萝卜籽肽的分离鉴定及活性表征 [J]. | 食品与发酵工业 , 2019 , 45 (2) : 95-100 . |
| MLA | 王惠敏 et al. "抗氧化胡萝卜籽肽的分离鉴定及活性表征" . | 食品与发酵工业 45 . 2 (2019) : 95-100 . |
| APA | 王惠敏 , 户佩 , 蔡甜甜 , 徐淑丽 , 洪晶 , 汪少芸 . 抗氧化胡萝卜籽肽的分离鉴定及活性表征 . | 食品与发酵工业 , 2019 , 45 (2) , 95-100 . |
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