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学者姓名：卢巧梅
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Abstract ：
As an emerging isoxazoline insecticide, afoxolaner has a good effect in the prevention and treatment of canine parasite infections. During the crystallization process of afoxolaner, inconsistent crystal phases and thermal properties are observed in the same batch of samples. In this work, the solubility and crystallization process were studied, and the best method was found to be slow evaporation in ethanol. The results showed that the crystal phase was highly consistent. The average peak value of DSC was observed at 149.6 +/- 0.6 degrees C, with a relative mean deviation of only 0.4%. Furthermore, the isolation of needle-like single crystals allowed for the determination of the crystal structure, revealing the enantiomeric pairs of afoxolaner co-crystallized with water in a monoclinic crystal system belonging to the centrosymmetric space group P21/n. It is hoped that this study will provide important insights into the crystallographic data of afoxolaner for its practical applications.
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| GB/T 7714 | Fang, Zong-Bin , Xiao, Yue-Feng , Sun, Chong et al. Solubility, crystallization process optimization, and thermal properties of afoxolaner and the single-crystal structure of its hydrate [J]. | CRYSTENGCOMM , 2024 , 27 (6) : 795-800 . |
| MLA | Fang, Zong-Bin et al. "Solubility, crystallization process optimization, and thermal properties of afoxolaner and the single-crystal structure of its hydrate" . | CRYSTENGCOMM 27 . 6 (2024) : 795-800 . |
| APA | Fang, Zong-Bin , Xiao, Yue-Feng , Sun, Chong , Chen, Xinyao , Zhou, Lingyu , Chen, Li et al. Solubility, crystallization process optimization, and thermal properties of afoxolaner and the single-crystal structure of its hydrate . | CRYSTENGCOMM , 2024 , 27 (6) , 795-800 . |
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Analytical screening and validation systems based on a combination of cell membrane chromatography and two-dimensional chromatography-tandem mass spectrometry are incapable of providing prepared samples containing the active ingredients found in traditional Chinese medicine; therefore, these samples cannot be directly used in subsequent studies. In this study, a semi-preparative cell membrane chromatography column was developed using a hydrogel-modified carrier and human umbilical vein endothelial cells to optimize prepared conditions, such as hydrogel polymerization, cell fragmentation, and cell membrane volume. This increased the binding ratio of membrane protein and carrier to 15.79 mg/g. The column was systematically evaluated using multitarget tyrosine kinase inhibitors that displayed good specificity and reproducibility. Subsequently, using the column coupled with a semi-preparative high-performance liquid chromatography-offline-high-performance liquid chromatography-mass spectrometry system, 15 active ingredients were screened and purified from Indigo naturalis, and five main components were identified: l-lysine, oxyresveratrol, tryptanthrin, isorhamnetin, and indirubin. Furthermore, the pharmacological effects of the ingredients were confirmed using cell proliferation and apoptosis assays. Results revealed potent proliferation-inhibiting and apoptosis-promoting abilities on human chronic myelogenous leukemic cells and human promyelocytic leukemic cells (p < 0.001). Overall, the system presented screening and purification functions that could be used to prepare I. naturalis samples acting on the epidermal growth factor receptor and vascular endothelial cell growth factor.
Keyword ：
cell membrane chromatography cell membrane chromatography human umbilical vein endothelial cell human umbilical vein endothelial cell hydrogel hydrogel purification screening system purification screening system semi-preparative high-performance liquid chromatography semi-preparative high-performance liquid chromatography
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| GB/T 7714 | Huang, Hui , Dai, Yabin , Zhang, Yuefen et al. System to screen and purify active ingredients from herbal medicines using hydrogel-modified human umbilical vein endothelial cell membrane chromatography coupled with semi-preparative high-performance liquid chromatography-offline-high-performance liquid chromatography-mass spectrometry [J]. | JOURNAL OF SEPARATION SCIENCE , 2023 , 46 (14) . |
| MLA | Huang, Hui et al. "System to screen and purify active ingredients from herbal medicines using hydrogel-modified human umbilical vein endothelial cell membrane chromatography coupled with semi-preparative high-performance liquid chromatography-offline-high-performance liquid chromatography-mass spectrometry" . | JOURNAL OF SEPARATION SCIENCE 46 . 14 (2023) . |
| APA | Huang, Hui , Dai, Yabin , Zhang, Yuefen , Li, Yongning , Ye, Huazhen , Guo, Dan et al. System to screen and purify active ingredients from herbal medicines using hydrogel-modified human umbilical vein endothelial cell membrane chromatography coupled with semi-preparative high-performance liquid chromatography-offline-high-performance liquid chromatography-mass spectrometry . | JOURNAL OF SEPARATION SCIENCE , 2023 , 46 (14) . |
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Sample pretreatment is an essential step in chromatographic analysis. Solid phase extraction (SPE) is a widely used sample pretreatment method. In SPE, the quality of the adsorbent directly affects the adsorption and enrichment efficiency of the target compounds as well as the sensitivity and selectivity of the pretreatment and subsequent analysis. Therefore, the selection and development of adsorbents has become a research hotspot. Microporous organic networks (MONs) are a novel type of covalent organic materials that are synthesized by the Sonogashira reaction of aromatic alkynes and aromatic halides. These networks have the advantages of modifiable structures, large specific surface areas, high porosity, and simple synthesis methods. This paper reviews the synthesis and functional modification methods of MONs, with an emphasis on their applications in sample pretreatment. Future development trends are also prospected. In terms of synthesis, the preparation methods for MON-based materials have progressed from reflux and solvothermal synthesis to room-temperature synthesis, the conditions of which tend to be milder and more efficient. In terms of functional modification, the introduction of macromolecules and active groups (including amino, hydroxyl, and carboxylic groups) can increase the selectivity and active sites of MON-based materials. The combination of MONs with Fe3O4, SiO2, and metal organic frameworks yields core-shell-structured MONs. Furtherly, they can be calcined and etched to form porous carbon structures or hollow multilayer materials. Functionalized MONs and their composite materials have multiple interaction mechanisms (e. g., hydrogen bonding, hydrophobic, electrostatic, and pi-pi interactions) with various target compounds, thereby realizing their efficient extraction. MONs can be used as adsorbent materials in SPE, Solid phase microextraction, dispersed solid phase extraction, magnetic solid phase extraction, and other pretreatment methods. When combined with chromatography and chromatography-mass spectrometry, MONs achieve good adsorption effects and high sensitivity, demonstrating the application potential of these materials in sample pretreatment.
Keyword ：
adsorbent adsorbent microporous organic networks (MONs) microporous organic networks (MONs) review review sample pretreatment sample pretreatment solid phase extraction solid phase extraction Sonogashira reaction Sonogashira reaction
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| GB/T 7714 | Yu Tao , Chen Li , Zhang Wenmin et al. Advances in synthesis methods and applications of microporous organic networks for sample preparation [J]. | CHINESE JOURNAL OF CHROMATOGRAPHY , 2023 , 41 (12) : 1052-1061 . |
| MLA | Yu Tao et al. "Advances in synthesis methods and applications of microporous organic networks for sample preparation" . | CHINESE JOURNAL OF CHROMATOGRAPHY 41 . 12 (2023) : 1052-1061 . |
| APA | Yu Tao , Chen Li , Zhang Wenmin , Zhang Lan , Lu Qiaomei . Advances in synthesis methods and applications of microporous organic networks for sample preparation . | CHINESE JOURNAL OF CHROMATOGRAPHY , 2023 , 41 (12) , 1052-1061 . |
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Covalent organic framework composites have received great interest and regarded as new-generation porous materials in application of sample preparation. In this work, an agaric-like graphene oxide @ co-valent organic framework (AGO@COF) composite was first synthesized and used as an ideal adsorbent for enrichment of trace cytokinins (CKs) due to its large specific surface area, rich in micro-mesopore and or-ganic functional groups. The contact areas between AGO@COF composite and CKs were greatly increased due to the special agaric-like morphology, which would further improve the extraction efficiency. Hence, a rapid, simple and efficient AGO@COF-based dispersive solid-phase extraction method for detecting four CKs (N 6-(delta 2-isopentenyl)-adenine, kinetin, DL-dihydrozeatin and 6-benzylaminopurine) was success-fully established. The proposed method had low limits of detection (0.1 -2.0 pg mL -1), good reproducibil-ity (RSDs <= 3.9%, n = 5) and high recoveries (84.4 -107.7%). With this method, N 6-(delta 2-isopentenyl)-adenine and 6-benzylaminopurine in plants were successfully detected and quantified. Results showed that the developed AGO@COF composite possessed superior extraction performance and potential appli-cation in enrichment of trace targets from complex plant samples. (c) 2022 Elsevier B.V. All rights reserved.
Keyword ：
Composite Composite Covalent organic framework Covalent organic framework Cytokinin Cytokinin Dispersive solid -phase extraction Dispersive solid -phase extraction HPLC-MS HPLC-MS MS MS
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| GB/T 7714 | Lu, Qiaomei , Lin, Shuting , Ding, Qingqing et al. An agaric-like covalent organic framework composite for efficient extraction of trace cytokinins in plant samples [J]. | JOURNAL OF CHROMATOGRAPHY A , 2022 , 1683 . |
| MLA | Lu, Qiaomei et al. "An agaric-like covalent organic framework composite for efficient extraction of trace cytokinins in plant samples" . | JOURNAL OF CHROMATOGRAPHY A 1683 (2022) . |
| APA | Lu, Qiaomei , Lin, Shuting , Ding, Qingqing , Zhang, Huiling , Tong, Ping , Fang, Min et al. An agaric-like covalent organic framework composite for efficient extraction of trace cytokinins in plant samples . | JOURNAL OF CHROMATOGRAPHY A , 2022 , 1683 . |
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A novel approach is presented to identify constituents with antileukemic properties in extracts of Indigo naturalis (Qingdai in Chinese). Target compounds (A(+), BC+, and ABC(+)) that knocked out specific constituents displayed antileukemic effects in a total extract of I. naturalis and negative constituents (A(-), BC-, and ABC(-)) that knocked out target compounds were separated, identified and knocked out by semipreparative liquid chromatography (semipreparative HPLC) and quadrupole time-of-flight mass spectrometer. Quantitative methods were used to evaluate the content of each knocked-out constituent in the total extract (D). Subsequently, interactions between the antileukemic effects of knocked-out constituents and D were screened and evaluated at the cellular level. Negative constituents including A(-) (65.47% +/- 1.20%), BC- (54.61% +/- 2.43%) and ABC(-) (67.49% +/- 3.28%) displayed a greater inhibitory effect than D (47.16% +/- 0.072%), which was not knocked out after 24 h of incubation, whereas the target compounds had not superior. Target compounds may have caused an antagonistic effect on the corresponding negative constituents. After 48 h, inhibition of proliferation by D (75.48% +/- 3.78%) increased compared with that by negative constituents, whereas the antagonistic effect of target components on negative constituents was diminished. This result may reflect competitive antagonism. Comparing the reactions after 24 and 48 h, the inhibitory ratio of ABC(-) (79.29% +/- 1.22%) in these knocked-out constituents and D was always the highest. With different concentrations tested after 48 h, ABC(-) significantly increased the rate of apoptosis on K562 cells (P < 0.01), indicating that in addition to indirubin, tryptanthrin and isorhamnetin, other antileukemic constituents may be present. Our study presents an approach that is a truer reflection of the antileukemic effects of knocked-out constituents in I. naturalis supported by reference to pharmacodynamic actions and the quality of I. naturalis. The approach may be useful for the analysis of other herbal extracts found in traditional Chinese medicine.
Keyword ：
antileukemic effect antileukemic effect constituent interactions constituent interactions Indigo naturalis (Qingdai) Indigo naturalis (Qingdai) LC-Q-TOF LC-Q-TOF quantitative analysis quantitative analysis semipreparative HPLC semipreparative HPLC target constituent knock-out target constituent knock-out
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| GB/T 7714 | Huang, Hui , Li, Yongning , Dai, Yabin et al. Antileukemic effects of indigo naturalis constituents by "target constituent knock-out" coupled with semipreparative liquid chromatography and quadrupole time-of-flight mass spectrometry [J]. | BIOMEDICAL CHROMATOGRAPHY , 2021 , 35 (12) . |
| MLA | Huang, Hui et al. "Antileukemic effects of indigo naturalis constituents by "target constituent knock-out" coupled with semipreparative liquid chromatography and quadrupole time-of-flight mass spectrometry" . | BIOMEDICAL CHROMATOGRAPHY 35 . 12 (2021) . |
| APA | Huang, Hui , Li, Yongning , Dai, Yabin , Zhang, Yuefen , Lu, Qiaomei , Xu, Qiaoxin et al. Antileukemic effects of indigo naturalis constituents by "target constituent knock-out" coupled with semipreparative liquid chromatography and quadrupole time-of-flight mass spectrometry . | BIOMEDICAL CHROMATOGRAPHY , 2021 , 35 (12) . |
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Frequent oil-spill accidents not only cause serious and long-term damage to marine ecosystems, but also lead to a huge loss of valuable natural resources. To lighten the environmental pollution of oil spills as quickly as possible, an efficient and environment-friendly approach for oil-water separation is highly desirable. Herein, a facile onepot room-temperature approach was developed for large-scale fabrication of covalent organic framework-coated superhydrophobic sponges (sponges@COFs). The as-prepared sponges@COFs possessed many superior properties, including superhydrophobicity with the water contact angle of approximately 154.3?, large specific surface area (153.059 m2/g), high porosity of the network structures, as well as good mechanical and chemical stability. Taking the aformentioned advantages together, the superhydrophobic sponges showed ultra-high adsorption capacity for oil and various organic solvents. In comparision with its own weight, the adsorption amount of the sponges@COFs for silicone oil was up to 150 times and for toluene was 125 times, respectively. Furthermore, the superhydrophobic sponges also showed fast and highly efficient oil-water separation, outstanding flame retardancy and recyclability. In addition, the sponges@COFs were successfully applied to the high-efficiency removal of oil suspension from industrial waste water, firmly confirming their application prospect in industrial wastewater treatment.
Keyword ：
Covalent organic framework Covalent organic framework Oil-water separation Oil-water separation One-pot room-temperature synthesis One-pot room-temperature synthesis Superhydrophobic sponges Superhydrophobic sponges
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| GB/T 7714 | Li, Jing , Yang, Yixin , Ma, Wende et al. One-pot room-temperature synthesis of covalent organic framework-coated superhydrophobic sponges for highly efficient oil-water separation [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2021 , 411 . |
| MLA | Li, Jing et al. "One-pot room-temperature synthesis of covalent organic framework-coated superhydrophobic sponges for highly efficient oil-water separation" . | JOURNAL OF HAZARDOUS MATERIALS 411 (2021) . |
| APA | Li, Jing , Yang, Yixin , Ma, Wende , Li, Guorong , Lu, Qiaomei , Lin, Zian . One-pot room-temperature synthesis of covalent organic framework-coated superhydrophobic sponges for highly efficient oil-water separation . | JOURNAL OF HAZARDOUS MATERIALS , 2021 , 411 . |
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植物激素在植物生长过程中具有重要作用,调节植物生长、发育及抗逆的各个过程.植物激素超微精准定量分析一直是植物生理学研究的瓶颈问题.植物激素的准确、高效检测目前大多是基于液相色谱-串联质谱联用技术.样品前处理是植物激素色谱-质谱分析中必不可少的一个步骤,直接影响后续检测方法的灵敏度和准确性.在植物激素各种前处理方法中,固相萃取(SPE)技术应用非常广泛.在萃取小柱基础上发展了多种新形式(分散固相萃取、磁性固相萃取、固相微萃取等,称之为SPE相关方法).在上述SPE相关方法中,吸附材料的选择均是关键因素,决定了样品前处理过程的目标物提取、净化和富集效果.碳基材料(包括碳纳米管、石墨烯、碳氮化合物等)和有机骨架材料(包括金属有机骨架、共价有机材料)拥有结构可设计、比表面积大、稳定性良好等特性,非常适合作为吸附材料.分子印迹聚合物和超分子化合物依靠主-客体特异性分子识别作用,能显著提高样品前处理方法的选择性.本文重点针对植物激素样品前处理中的SPE技术,综述了近5年来上述几类功能化吸附材料的最新应用进展,并对其发展趋势进行展望.
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| GB/T 7714 | 林舒婷 , 丁青青 , 张文敏 et al. 固相萃取吸附材料在植物激素样品前处理中的应用新进展 [J]. | 色谱 , 2021 , 39 (12) : 1281-1290 . |
| MLA | 林舒婷 et al. "固相萃取吸附材料在植物激素样品前处理中的应用新进展" . | 色谱 39 . 12 (2021) : 1281-1290 . |
| APA | 林舒婷 , 丁青青 , 张文敏 , 张兰 , 卢巧梅 . 固相萃取吸附材料在植物激素样品前处理中的应用新进展 . | 色谱 , 2021 , 39 (12) , 1281-1290 . |
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Fast and highly efficient digestion of proteins is essential for high-throughput proteomic analysis. Herein, a facile approach was developed for self-assembly preparation of trypsin-immobilized capillary monolithic column and its application as an immobilized enzyme microreactor (IMER) for fast and highly efficient proteolysis was described. The performance of the trypsin-immobilized monolithic enzyme microreactor was evaluated by in-situ digestion of model proteins. The results showed that the trypsinimmobilized monolithic enzyme microreactor had much higher tryptic digestion efficiency than the free trypsin in solution, where the coverage of peptide sequences by mass spectrometry (MS)-based analysis could bear comparison with the free one, while the digestion time was dramatically shortened from 12 h to 16 s. Furthermore, the trypsin-immobilized monolithic enzyme microreactor also exhibited good practicability to complex human serum sample, in which the total of 45 peptides from human serum albumin (HSA) matched with sequence coverage of 75% were precisely identified. The successful application demonstrated the promising potential of the trypsin-immobilized capillary monolithic column as the IMER in high-throughput proteomic analysis. (C) 2020 Elsevier B.V. All rights reserved.
Keyword ：
Capillary monolithic column Capillary monolithic column Protein digestion Protein digestion Self-assembly Self-assembly Trypsin-immobilized enzyme microreactor Trypsin-immobilized enzyme microreactor
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| GB/T 7714 | Zhong, Chao , Yang, Baichuan , Huang, Weini et al. Self-assembly synthes is of trypsin-immobilized monolithic microreactor for fast and efficient proteolysis [J]. | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1635 . |
| MLA | Zhong, Chao et al. "Self-assembly synthes is of trypsin-immobilized monolithic microreactor for fast and efficient proteolysis" . | JOURNAL OF CHROMATOGRAPHY A 1635 (2021) . |
| APA | Zhong, Chao , Yang, Baichuan , Huang, Weini , Huang, Huan , Zhang, Shasha , Yan, Xi et al. Self-assembly synthes is of trypsin-immobilized monolithic microreactor for fast and efficient proteolysis . | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1635 . |
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Sorbents with high surface utilization and good dispersibility are of great importance for the extraction performance of magnetic solid-phase extraction (MSPE). In this study, a fish scale-like magnetic nanomaterial (Co@Co3O4/OCN) was synthesized, which can be used as a highly efficient MSPE sorbent due to its strong magnetism, special morphology, doping of N element, numerous micro-mesopore cavities and organic functional groups (hydroxyl and carboxyl). Furthermore, a Co@Co3O4/OCN-based MSPE method for monitoring the changes in the levels of three auxins (indole-3-acetic acid, indole-3-propionic acid and 3-indole butyric acid) was successfully established. Wide linear ranges (1.0-1000.0 pg mL(-1)) with good correlation coefficients (R> 0.9992), low limits of detection (LODs, 0.2-4.0 pg mL(-1)) and satisfactory repeatability (RSD <= 5.9%,n= 3) were obtained. Using the developed method, various growth parts and different growth periods of plants under Cd stress were monitored. The results showed that auxins in various parts of plants showed differential response under Cd stress, and there was a threshold for the changes in auxin levels against Cd stress. This indicates that the developed fish scale-like Co@Co3O4/OCN nanomaterial has a good application prospect for enriching small molecular targets containing hydroxyl and carboxyl groups.
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| GB/T 7714 | Ding, Qingqing , Chen, Hui , Huang, Chuanhui et al. A fish scale-like magnetic nanomaterial as a highly efficient sorbent for monitoring the changes in auxin levels under cadmium stress [J]. | ANALYST , 2020 , 145 (17) : 5925-5932 . |
| MLA | Ding, Qingqing et al. "A fish scale-like magnetic nanomaterial as a highly efficient sorbent for monitoring the changes in auxin levels under cadmium stress" . | ANALYST 145 . 17 (2020) : 5925-5932 . |
| APA | Ding, Qingqing , Chen, Hui , Huang, Chuanhui , Lu, Qiaomei , Tong, Ping , Zhang, Wenmin et al. A fish scale-like magnetic nanomaterial as a highly efficient sorbent for monitoring the changes in auxin levels under cadmium stress . | ANALYST , 2020 , 145 (17) , 5925-5932 . |
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Fast and high-efficiency protein digestion is essential for high-throughput proteome analysis. Herein, a new kind of trypsin-inorganic hybrid nanocomposites were room-temperature synthesized based on self-assembly stragety, where trypsin and nickel phosphate (Ni-3(PO4)(2)) was used as organic and inorganic ligands, respectively. The general applicability of the synthetic method was also confirmed by using a series of other metal phosphates including Mg2+, Ba2+, Zn2+, and Co2+. The as-prepared trypsin-inorganic hybrid nanocomposites with flower-like morphology showed higher enzymatic acticity (206 % enhancement) and faster catalytic kinetics than the free trypsin. Taking the merits togethers as mentioned above, the hybrid nanocomposites could be employed as an immmobilized enzyme reactor (IMER) for fast and high-efficiency protein digestion. The peptide mass fingerprint analysis of the model proteins demonstrated that the coverage rate of the sequences treated by IMER could bear comparsion with that of the free one, but the digestion time was shorten from 12 h to 1 min. In addition, the IMER showed a good applicability for complex human serum sample, showing a promising potential in proteomics research.
Keyword ：
immobilization immobilization proteins digestion proteins digestion proteomic proteomic self-assembly self-assembly trypsin-Ni-3(PO4)(2) hybrid nanocomposites trypsin-Ni-3(PO4)(2) hybrid nanocomposites
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| GB/T 7714 | Lin, Juan , Zhong, Chao , Lu, Qiaomei et al. Room-Temperature Synthesis of Trypsin-Inorganic Hybrid Nanocomposites for Fast and Efficient Protein Digestion [J]. | CHEMISTRYSELECT , 2020 , 5 (40) : 12500-12504 . |
| MLA | Lin, Juan et al. "Room-Temperature Synthesis of Trypsin-Inorganic Hybrid Nanocomposites for Fast and Efficient Protein Digestion" . | CHEMISTRYSELECT 5 . 40 (2020) : 12500-12504 . |
| APA | Lin, Juan , Zhong, Chao , Lu, Qiaomei , Lin, Zian . Room-Temperature Synthesis of Trypsin-Inorganic Hybrid Nanocomposites for Fast and Efficient Protein Digestion . | CHEMISTRYSELECT , 2020 , 5 (40) , 12500-12504 . |
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