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学者姓名：何小镇
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Abstract ：
Background The outer membrane vesicles (OMVs) derived from Porphyromonas gingivalis (P. gingivalis) have long been acknowledged for their crucial role in the initiation of periodontitis. However, the implications of P. gingivalis OMVs in the context of cardiovascular disease (CVD) remain incompletely understood. This study aimed to clarify both the impact and the underlying mechanisms through which P. gingivalis OMVs contribute to the propagation of distal cardiovascular inflammation and trauma.Methods In this study, various concentrations (0, 1.25, 2.5, and 4.5 mu g/mu L) of P. gingivalis OMVs were microinjected into the common cardinal vein of zebrafish larvae at 48 h post-fertilization (hpf) to assess changes in cardiovascular injury and inflammatory response. Zebrafish larvae from both the PBS and the 2.5 mu g/mu L injection cohorts were harvested at 30 h post-injection (hpi) for transcriptional analysis. Real-time quantitative PCR (RT-qPCR) was employed to evaluate relative gene expression.Results These findings demonstrated that P. gingivalis OMVs induced pericardial enlargement in zebrafish larvae, caused vascular damage, increased neutrophil counts, and activated inflammatory pathways. Transcriptomic analysis further revealed the involvement of the immune response and the extracellular matrix (ECM)-receptor interaction signaling pathway in this process.Conclusion This study illuminated potential mechanisms through which P. gingivalis OMVs contribute to CVD. It accentuated their involvement in distal cardiovascular inflammation and emphasizes the need for further research to comprehensively grasp the connection between periodontitis and CVD.
Keyword ：
Cardiovascular disease Cardiovascular disease Outer membrane vesicles Outer membrane vesicles Periodontitis Periodontitis Porphyromonas gingivalis Porphyromonas gingivalis Transcriptome Transcriptome
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| GB/T 7714 | Guo, Jianbin , Lin, Kaijin , Wang, Siyi et al. Effects and mechanisms of Porphyromonas gingivalis outer membrane vesicles induced cardiovascular injury [J]. | BMC ORAL HEALTH , 2024 , 24 (1) . |
| MLA | Guo, Jianbin et al. "Effects and mechanisms of Porphyromonas gingivalis outer membrane vesicles induced cardiovascular injury" . | BMC ORAL HEALTH 24 . 1 (2024) . |
| APA | Guo, Jianbin , Lin, Kaijin , Wang, Siyi , He, Xiaozhen , Huang, Zhen , Zheng, Minqian . Effects and mechanisms of Porphyromonas gingivalis outer membrane vesicles induced cardiovascular injury . | BMC ORAL HEALTH , 2024 , 24 (1) . |
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目的 建立一种高特异性人乳头瘤病毒(HPV)分型检测的荧光定量聚合酶链式反应方法.方法 设计针对23种HPV分型的特异性引物和小沟凹槽(MGB)探针,优化引物、探针序列及反应体系,利用中国食品药品检定研究院公布的第二代HPV全基因组分型国家参考品验证其有效性、灵敏度、精密度和交叉特异性,并将其应用于已确定亚型的患者宫颈脱落细胞筛查.结果 23种引物和探针均通过有效性、灵敏度、交叉特异性及精密度的检验,最低检出限达100拷贝/μL,变异系数≤3.18％.在114份临床样品的筛查中,100份为阳性,14份为阴性,均与已知结果相符.结论 本研究建立的分型检测体系能特异性检测23种HPV并对其进行基因分型,可作为一个候选的理想检测方法,为临床HPV感染诊断提供有效参考.
Keyword ：
人乳头瘤病毒 人乳头瘤病毒 小沟凹槽探针 小沟凹槽探针 荧光定量聚合酶链式反应 荧光定量聚合酶链式反应
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| GB/T 7714 | 黄妍婷 , 刘继来 , 高廷芳 et al. 基于小沟凹槽探针特异性检测23种人乳头瘤病毒分型的研究 [J]. | 疾病监测 , 2024 , 39 (11) : 1477-1483 . |
| MLA | 黄妍婷 et al. "基于小沟凹槽探针特异性检测23种人乳头瘤病毒分型的研究" . | 疾病监测 39 . 11 (2024) : 1477-1483 . |
| APA | 黄妍婷 , 刘继来 , 高廷芳 , 许万里 , 柯文雅 , 何小镇 et al. 基于小沟凹槽探针特异性检测23种人乳头瘤病毒分型的研究 . | 疾病监测 , 2024 , 39 (11) , 1477-1483 . |
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Human lymphocytes as well as zebrafish embryos were used to study the toxicity of acrylamide, and the intervention effects of the dietary antioxidants (-)-epigallocatechin gallate and curcumin have been examined. The alkaline comet assay revealed that acrylamide (1, 5, 10 mmol/L) had dose-response effects on basic DNA damage and oxidative DNA damage in human lymphocytes. Exposure of zebrafish embryos to acrylamide resulted in decrease of hatching rate, and in increases of mortality and teratogenicity. (-)-Epigallocatechin gallate and curcumin reduced DNA damage and protected zebrafish embryos in the growth and development.
Keyword ：
Acrylamide Acrylamide curcumin curcumin developmental toxicity developmental toxicity (-)- epigallocatechin gallate (-)- epigallocatechin gallate genotoxicity genotoxicity human lymphocytes human lymphocytes zebrafish embryos zebrafish embryos
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| GB/T 7714 | Wang, Shanshan , Wang, Hufei , Chen, Yanqiu et al. Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity [J]. | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY , 2021 , 103 (2) : 199-218 . |
| MLA | Wang, Shanshan et al. "Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity" . | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY 103 . 2 (2021) : 199-218 . |
| APA | Wang, Shanshan , Wang, Hufei , Chen, Yanqiu , Liu, Jilai , He, Xiaozhen , Huang, Da et al. Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity . | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY , 2021 , 103 (2) , 199-218 . |
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As a vertebrate model, zebrafish (Danio rerio) plays a vital role in the field of life sciences. Recently, gene-editing technology has become increasingly innovative, significantly promoting scientific research on zebrafish. However, the implementation of these methods in a reasonable and accurate manner to achieve efficient gene-editing remains challenging. In this review, we systematically summarize the development and latest progress in zebrafish gene-editing technology. Specifically, we outline trends in double-strand break-free genome modification and the prospective applications of fixed-point orientation transformation of any base at any location through a multi-method approach.
Keyword ：
Danio rerio Danio rerio double-stranded break double-stranded break fixed-point orientation transformation fixed-point orientation transformation gene editing gene editing genome modification genome modification nick nick vertebrate model vertebrate model zebrafish zebrafish
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| GB/T 7714 | Li, Yanling , Jia, Zhipeng , Zhang, Shuchao et al. Progress in Gene-Editing Technology of Zebrafish [J]. | BIOMOLECULES , 2021 , 11 (9) . |
| MLA | Li, Yanling et al. "Progress in Gene-Editing Technology of Zebrafish" . | BIOMOLECULES 11 . 9 (2021) . |
| APA | Li, Yanling , Jia, Zhipeng , Zhang, Shuchao , He, Xiaozhen . Progress in Gene-Editing Technology of Zebrafish . | BIOMOLECULES , 2021 , 11 (9) . |
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提供了一种在鱼胚胎中精确剪切位点特异性RNA的技术， 采用CaSrx介导的RNA编辑技术，只需提供CaSrx mRNA和特异性靶向引导RNA，即可实现对鱼胚胎中位点特异性RNA的精确敲除，具有高效，特异性强，方便，成本低等诸多优点。 本发明克服了现有siRNA技术效率低，吗啉代介导的基因敲除技术成本高，效果不理想的问题。
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| GB/T 7714 | He Xiaozhen , Zhong Lili . 一种精确剪切鱼胚胎中位点特异性RNA的技术 : ZA202110568[P]. | 2021-12-17 . |
| MLA | He Xiaozhen et al. "一种精确剪切鱼胚胎中位点特异性RNA的技术" : ZA202110568. | 2021-12-17 . |
| APA | He Xiaozhen , Zhong Lili . 一种精确剪切鱼胚胎中位点特异性RNA的技术 : ZA202110568. | 2021-12-17 . |
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Site-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome editing strategy we named as NEO, which used either site-specific trans or cis double-nicking facilitated by four bacterial recombination factors (RecOFAR). In comparison to currently available approaches, NEO achieved higher knock-in (KI) germline transmission frequency (improving from zero to up to 10% efficiency with an average of 5-fold improvement for 8 loci) and 'cleaner' knock-in of long DNA fragments (up to 5.5 kb) into a variety of genome regions in zebrafish, mice and rats. Furthermore, NEO yielded up to 50% knock-in in monkey embryos and 20% relative integration efficiency in non-dividing primary human peripheral blood lymphocytes (hPBLCs). Remarkably, both on-target and off-target indels were effectively suppressed by NEO. NEO may also be used to introduce low-risk unrestricted point mutations effectively and precisely. Therefore, by balancing efficiency with safety and quality, the NEO method reported here shows substantial potential and improves the in vivo gene-editing strategies that have recently been developed.
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| GB/T 7714 | He, Xiaozhen , Chen, Wenfeng , Liu, Zhen et al. Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species [J]. | NUCLEIC ACIDS RESEARCH , 2020 , 48 (10) . |
| MLA | He, Xiaozhen et al. "Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species" . | NUCLEIC ACIDS RESEARCH 48 . 10 (2020) . |
| APA | He, Xiaozhen , Chen, Wenfeng , Liu, Zhen , Yu, Guirong , Chen, Youbang , Cai, Yi-Jun et al. Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species . | NUCLEIC ACIDS RESEARCH , 2020 , 48 (10) . |
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