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学者姓名：吴元子
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Abstract ：
Zearalenone (ZEN) is a common contaminant in crops with serious food safety implications. By leveraging the etching effect of H2O2 on gold nanorods (Au NRs) and the catalysis of Fenton reaction, we have successfully developed two enzyme-linked immunosorbent assay (ELISA) kits with distinct principles. ZEN can be accurately quantified by both methods by measuring changes in the longitudinal surface plasmon resonance (LSPR) absorption peaks of Au NRs. Direct detection kit is simpler and faster, with a linear range of 0.2-4000 ng/mL, while indirect competitive kit is more complex and has a narrower linear range of 0.02-10 ng/mL. This study is expected to provide an effective analytical strategy for rapid screening and accurate monitoring of mold contaminants in food and agricultural products.In this study, a novel dual-mode ELISA system was developed for the detection of zearalenone (ZEN) using Au NRs and their longitudinal surface plasmon resonance (LSPR) properties. By integrating H2O2-mediated nano-etching and Fenton reaction catalysis, the system enables both direct (0.2-4000 ng/mL) and indirect competitive (0.02-10 ng/mL) detection modes. The main work includes: (1) replacing the traditional enzyme colorimetric signal with an LSPR shift for accurate UV-visible quantification and visual multicolor readout; (2) optimizing the nanoenzyme catalytic system for improved reaction sensitivity; (3) eliminating the need for enzyme-labeled secondary antibodies in direct mode, thereby reducing cost and improving stability. The system was validated on grain samples with recoveries of 93.1-107.1% and detection limits of 0.2 ng/mL (direct) and 0.02 ng/mL (indirect). The modular design of the method allows it to be used for the detection of other mycotoxins (e.g., aflatoxin, ochratoxin) and offers great potential for food safety screening, agricultural monitoring, and environmental assessment. This work provides a cost-effective, sensitive and versatile platform for mycotoxin analysis.
Keyword ：
Food safety Food safety Gold nanorods Gold nanorods Visual detection Visual detection Zearalenone Zearalenone
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| GB/T 7714 | Hu, Yuanlong , Chen, Jiamin , Wu, Sifang et al. A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods [J]. | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION , 2025 , 19 (9) : 6665-6676 . |
| MLA | Hu, Yuanlong et al. "A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods" . | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION 19 . 9 (2025) : 6665-6676 . |
| APA | Hu, Yuanlong , Chen, Jiamin , Wu, Sifang , Zhong, Yi , Ye, Jialin , Ye, Lingqing et al. A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods . | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION , 2025 , 19 (9) , 6665-6676 . |
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The rise of antibiotic-resistant bacteria poses a serious global health threat, highlighting the urgent need for novel strategies beyond conventional antibiotic therapies. This study explores the potential of microbe-imprinted polymers (MIPs) as innovative, pathogen-specific affinity agents. Utilizing microbial surface-initiated polymerization, MIPs are in-situ synthesized on the surface of target microbes, creating flexible heteropolymers that precisely replicate microbial surface structures. This method exhibits high affinity (KD = 2.7x108 CFU/mL for E. coli) and selectivity at the strain level. MIPs offer significant advantages over traditional antibodies, including greater stability, cost-effectiveness, and a broader spectrum of binding capabilities, making them effective for identifying and targeting various microbial strains, including unidentified or drug-resistant variants. Moreover, their favorable biocompatibility and functional resilience in diverse environments position MIPs as promising candidates for rapid pathogen detection and therapeutic applications. This research paves the way for advanced biomimetic materials in microbe-specific diagnostics and combating infections, addressing the critical need for effective tools in antibiotic resistance surveillance.
Keyword ：
Affinity Affinity Antibiotic resistance Antibiotic resistance Antibody mimics Antibody mimics Microbe-imprinted polymers Microbe-imprinted polymers Microbial recognition Microbial recognition
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| GB/T 7714 | Wu, Yuanzi , Zhou, Kaiqiang , Li, Wenhui et al. Microbe-imprinted polymers for rapid drug-resistant bacteria recognition [J]. | CHEMICAL ENGINEERING JOURNAL , 2025 , 512 . |
| MLA | Wu, Yuanzi et al. "Microbe-imprinted polymers for rapid drug-resistant bacteria recognition" . | CHEMICAL ENGINEERING JOURNAL 512 (2025) . |
| APA | Wu, Yuanzi , Zhou, Kaiqiang , Li, Wenhui , Huan, Min , Yu, Zhichao , Yan, Fen et al. Microbe-imprinted polymers for rapid drug-resistant bacteria recognition . | CHEMICAL ENGINEERING JOURNAL , 2025 , 512 . |
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Formaldehyde (FA), a known carcinogen, is occasionally used illegally as a preservative in seafood, while traditional detection methods for FA residues often fail to meet the practical needs for nondestructive detection. In this study, a approach was developed by combining a portable Raman spectrometer with the InceptionTime deep learning model without sample pretreatment. Model were trained by FA-negative and FA-positive Raman spectral data from the shrimp surface and achieved accuracies of 84.40 % and 85.17 % at detection thresholds of 5 mg/kg (the primary safety detection threshold) and 100 mg/kg (the abuse-level contamination threshold), respectively. Metabolomic analysis and weight visualization indicated that the model particularly focused on Raman peaks associated with specific amino acids and astaxanthin-binding proteins. Two amino acid metabolites, timonacic and spinacine, were also identified as direct indicators of FA addition. Our model offers a fielddeployable and practical approach for real-time and on-site FA detection scenario.
Keyword ：
Deep learning Deep learning Formaldehyde detection Formaldehyde detection Nondestructive detection Nondestructive detection Raman spectroscopy Raman spectroscopy Shrimp Shrimp
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| GB/T 7714 | Wei, Chencheng , Zhang, Jiheng , Li, Gaozheng et al. Rapid and non-destructive detection of formaldehyde adulteration in shrimp based on deep learning-assisted portable Raman spectroscopy [J]. | FOOD CHEMISTRY , 2025 , 492 . |
| MLA | Wei, Chencheng et al. "Rapid and non-destructive detection of formaldehyde adulteration in shrimp based on deep learning-assisted portable Raman spectroscopy" . | FOOD CHEMISTRY 492 (2025) . |
| APA | Wei, Chencheng , Zhang, Jiheng , Li, Gaozheng , Zhong, Yi , Ye, Zhaoting , Wang, Handong et al. Rapid and non-destructive detection of formaldehyde adulteration in shrimp based on deep learning-assisted portable Raman spectroscopy . | FOOD CHEMISTRY , 2025 , 492 . |
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Enzymes are crucial for various technological applications, but their inherent instability and short lifespan pose challenges. This study presents facile immobilized enzyme technology with the development of thermoresponsive enzyme-polymer conjugates (EPCs), using glucose oxidase (GOx) as a model enzyme, to address these limitations. By conjugating heteropolymers to the glycan moieties of GOx through a precise in situ polymerization process, we could modulate the lower critical solution temperature of the EPCs, enhancing enzyme performance without compromising its active site. The EPCs demonstrate a switchable behavior that facilitates efficient homogeneous catalysis and easy heterogeneous separation, reducing costs and environmental impact in industrial applications. Our strategy presents a versatile platform for creating efficient biocatalysts with tunable properties, marking a step forward in sustainable and cost-effective bioprocessing.
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| GB/T 7714 | Wang, Huiru , Ma, Shanyun , Diao, Muyan et al. Engineering Thermoresponsive Enzyme-Polymer Conjugates via Glycan-Selective In Situ Polymerization for Recyclable Homogeneous Biocatalysis [J]. | BIOMACROMOLECULES , 2024 , 25 (12) : 7951-7957 . |
| MLA | Wang, Huiru et al. "Engineering Thermoresponsive Enzyme-Polymer Conjugates via Glycan-Selective In Situ Polymerization for Recyclable Homogeneous Biocatalysis" . | BIOMACROMOLECULES 25 . 12 (2024) : 7951-7957 . |
| APA | Wang, Huiru , Ma, Shanyun , Diao, Muyan , Li, Wenhui , Huan, Min , Sun, Xiaofang et al. Engineering Thermoresponsive Enzyme-Polymer Conjugates via Glycan-Selective In Situ Polymerization for Recyclable Homogeneous Biocatalysis . | BIOMACROMOLECULES , 2024 , 25 (12) , 7951-7957 . |
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Inflammation is a critical immune response to various stimuli but can lead to diseases when uncontrolled. Commonly used anti-inflammatory drugs usually associated with side effects, natural products such as curcumin (CUR) have emerged as a promising alternative but suffer from low bioavailability. This study introduces a novel nanocarrier, amphiphilic hyperbranched polyprodrug (P(ACPP-co-CURMA)-b-POEGMA, PACE), designed to enhance the anti-inflammatory activity of CUR. PACE micelles, formed from a hydrophobic hyperbranched polyprodrug core and hydrophilic POEGMA corona, demonstrated remarkable stability, even upon dilution, and efficiently released CUR in response to the reductive intracellular environment. These micelles were biocompatible and effectively internalized by macrophages. In both in vitro and in vivo studies, PACE micelles demonstrated significant anti-inflammatory activity, reducing ROS levels, down-regulating pro-inflammatory cytokines, and alleviating inflammation. This work highlights the potential of PACE micelles as a promising platform for targeted and responsive anti-inflammatory therapy, with implications for treating inflammatory disorders.
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| GB/T 7714 | Huang, Da , Tang, Qiang , Zou, Minglang et al. Anti-inflammatory unimolecular micelles of redox-responsive hyperbranched polycurcumin amphiphiles with enhanced anti-inflammatory efficacy in vitro and in vivo [J]. | POLYMER CHEMISTRY , 2023 , 14 (47) : 5208-5217 . |
| MLA | Huang, Da et al. "Anti-inflammatory unimolecular micelles of redox-responsive hyperbranched polycurcumin amphiphiles with enhanced anti-inflammatory efficacy in vitro and in vivo" . | POLYMER CHEMISTRY 14 . 47 (2023) : 5208-5217 . |
| APA | Huang, Da , Tang, Qiang , Zou, Minglang , Wang, Yongming , Luo, Fang , Mu, Jinyi et al. Anti-inflammatory unimolecular micelles of redox-responsive hyperbranched polycurcumin amphiphiles with enhanced anti-inflammatory efficacy in vitro and in vivo . | POLYMER CHEMISTRY , 2023 , 14 (47) , 5208-5217 . |
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Non-alcoholic fatty liver disease (NAFLD) has become a globally rising issue that can cause liver-related morbidity and mortality. Recent studies have revealed that a high-fat diet (HFD) highly contributes to the prevalence and progression of NAFLD via impacting gut microbiota and lipid metabolism signal pathways. Resveratrol (RSV), a natural bioactive compound, has exhibited potential for preventing and alleviating NAFLD. However, due to the poor bioavailability of RSV, its strengths and underlying mechanisms for NAFLD therapeutic potential are poorly understood. To address this, we utilized Hydroxypropyl-beta-Cyclodextrin (HBC) to encapsulate RSV to enhance its water solubility and conducted prevention and intervention experiments in HFDfed mice. The results showed that the HBC has significantly enhanced the water solubility of RSV by 250-fold and the HBC-RSV better prevented and alleviated the liver steatosis, obesity and abnormal lipid metabolism induced by HFD than RSV alone. Meanwhile, combining HFD and HBC-RSV or RSV prevented HFD mice progressing to NAFLD. Besides, further investigation indicated that RSV could resist liver injury and obesity by modulating gut microbiota, raising the levels of short-chain fatty acids (SCFAs) and activating AMP-activated protein kinase (AMPK) signaling pathway. The activated pathway down-regulated sterol receptor element binding protein 1c (SREBP1c) and acetyl-coenzyme A carboxylase (ACC) to decrease lipid synthesis and up-regulated peroxisome proliferators-activated receptor & alpha; (PPAR & alpha;) to promote the fatty acid oxidation, thus preventing NAFLD. Our findings suggested that water solubility-enhanced RSV beneficially modulated gut microbiota, altered gut microbiota-derived SCFAs, and activated lipid metabolism regulatory pathways, providing potential for NAFLD prevention and alleviation.
Keyword ：
AMPK AMPK Gut microbiota Gut microbiota Hydroxypropyl-beta-Cyclodextrin Hydroxypropyl-beta-Cyclodextrin NAFLD NAFLD resveratrol resveratrol SCFAs SCFAs
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| GB/T 7714 | Ke, Wenya , Huang, Juan , Zhong, Yi et al. Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway [J]. | FOOD BIOSCIENCE , 2023 , 54 . |
| MLA | Ke, Wenya et al. "Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway" . | FOOD BIOSCIENCE 54 (2023) . |
| APA | Ke, Wenya , Huang, Juan , Zhong, Yi , Shi, Yuhong , Yan, Fen , Huang, Da et al. Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway . | FOOD BIOSCIENCE , 2023 , 54 . |
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Bacterial infection of wounds is one of great concern to patients, and rapid and correct detection of bacterial infection is crucial to ensure accurate diagnosis and early intervention. Based on the principle that glucose can only be consumed by live bacteria, a fluorescent biosensor was constructed to detect four kinds of common infectious bacteria (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) in skin wounds, taking advantage of magnetic bead-aptamer for recognizing, sorting and enrichment, platinum nanoparticles for signal amplification. The linear detection range of AB, EC, PA and SA were 27- 8 x 106 CFU/mL, 10- 2.5 x 107 CFU/mL, 34- 2.5 x 107 CFU/mL and 10- 1.0 x 107 CFU/mL, respectively, and the limits of detection were 27 CFU/mL, 10 CFU/mL, 34 CFU/mL and 2 CFU/mL. Furthermore, all target bacteria in samples containing 8 x 108 CFU/mL of other interfering bacteria have been successfully identified and quantified. The proposed method was also successfully applied to the detection of bacterial infection in skin wounds of mouse and human, including the detection of separate bacterial infection as well as a coinfection. The recovery of this method was in the range of 90.161- 109.961%. Thus, this proposed method can be a promising candidate for rapid and convenient evaluation of infectious bacteria in point-of-care settings.
Keyword ：
Aptamer Aptamer Bacterial infection Bacterial infection Fluorescent biosensor Fluorescent biosensor Platinum nanoparticles Platinum nanoparticles Wound Wound
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| GB/T 7714 | Gao, Lanmei , Zheng, Houbing , Hu, Yuanlong et al. Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
| MLA | Gao, Lanmei et al. "Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds" . | SENSORS AND ACTUATORS B-CHEMICAL 393 (2023) . |
| APA | Gao, Lanmei , Zheng, Houbing , Hu, Yuanlong , Zhong, Yi , Jiang, Linhai , Wu, Yuanzi et al. Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds . | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
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In view of the biological significance and micro-heterogeneity of protein glycosylation for human health, specific enrichment of N-glycosylated proteins/peptides from complex biological samples is a prerequisite for the discovery of disease biomarkers and clinical diagnosis. In this work, we propose a "grafting-from" N-glycoprotein enriching method based on the in-situ growth of thermoresponsive polymer brushes from the N-glycosylated site of proteins. The initiator was first attached to the pre-oxidized glycan moieties by hydrazide chemistry, from which the thermoresponsive polymers can be grown to form giant protein-polymer conjugates (PPC). The thermosensitive PPC can be precipitated and separated by raising the temperature to above its lower critical solubility temperature (LCST). Mass spectrometry verified 210 N-glycopeptides corresponding to 136 N-glycoproteins in the rabbit serum. These results demonstrate the capability of the tandem thermoprecipitation strategy to enrich and separate N-glycoprotein/glycopeptide. Due to its simplicity and efficiency specifically, this method holds the potential for identifying biomarkers from biological samples in N-glycoproteome analysis.
Keyword ：
Glycan-selective Glycan-selective glycopeptide glycopeptide N-Glycoprotein N-Glycoprotein Thermoprecipitation Thermoprecipitation
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| GB/T 7714 | Shu, Jingjing , Xiong, Wenli , Zhang, Ran et al. Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides [J]. | TALANTA , 2023 , 253 . |
| MLA | Shu, Jingjing et al. "Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides" . | TALANTA 253 (2023) . |
| APA | Shu, Jingjing , Xiong, Wenli , Zhang, Ran , Ma, Shanyun , Zhou, Kaiqiang , Wang, Xuwei et al. Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides . | TALANTA , 2023 , 253 . |
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An abnormal microenvironment underlies poor healing in chronic diabetic chronic wounds. However, effectively modulating the microenvironment of the diabetic wound remains a great challenge due to sustained oxidative stress and chronic inflammation. Here, we present a unimolecular enzyme-polymer conjugate that demonstrates excellent multienzymatic cascade activities. The cascaded enzyme conjugates (CECs) were synthesized by grafting poly(N-acryloyl-lysine) (pLAAm) from the glycan moieties of glucose oxidase (GOx) via glycan-initiated polymerization. The resulting CECs exhibited multiple enzymatic properties of GOx, superoxide dismutase mimic, and catalase mimic activities simultaneously. The CECs facilitated the depletion of high blood glucose, ROS scavenging, bacteria-killing, anti-inflammatory effects, and sustained oxygen generation, which restored the microenvironment in diabetic wounds. In vivo results from a diabetic mouse model confirmed the capacity and efficiency of the cascade reaction for diabetic wound healing. Our findings demonstrate that the three-in-one enzyme-polymer conjugates alone can modulate the diabetic microenvironment for wound healing.
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| GB/T 7714 | Wu, Yuanzi , Lyu, Yanwei , Li, Ling et al. Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing [J]. | BIOMACROMOLECULES , 2023 , 25 (1) : 43-54 . |
| MLA | Wu, Yuanzi et al. "Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing" . | BIOMACROMOLECULES 25 . 1 (2023) : 43-54 . |
| APA | Wu, Yuanzi , Lyu, Yanwei , Li, Ling , Zhou, Kaiqiang , Cai, Jingwen , Wang, Xuwei et al. Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing . | BIOMACROMOLECULES , 2023 , 25 (1) , 43-54 . |
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本发明提供一种无泄漏封包酵母水凝胶及其制备方法。通过构建携带人源基因的酵母工程菌，所得工程酵母菌表面糖链进行原位氧化，引发，高分子的特异性交联制备获得无泄漏封包酵母水凝胶。该水凝胶可防止酵母菌的泄露，减小直接接触人体时可能产生的免疫反应并加速伤口愈合。本发明的优点：生物相容性佳，反应温和，应用前景广泛。
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| GB/T 7714 | 吴元子 , 蔡静雯 , 舒京晶 et al. 一种无泄漏封包酵母水凝胶及其制备方法 : CN202210103555.3[P]. | 2022-01-28 . |
| MLA | 吴元子 et al. "一种无泄漏封包酵母水凝胶及其制备方法" : CN202210103555.3. | 2022-01-28 . |
| APA | 吴元子 , 蔡静雯 , 舒京晶 , 李玲 , 周凯强 , 王旭伟 et al. 一种无泄漏封包酵母水凝胶及其制备方法 : CN202210103555.3. | 2022-01-28 . |
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