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学者姓名：黄彦
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Abstract ：
The associative phase separation of charged biomacromolecules plays a key role in many biophysical events that take place in crowded intracellular environments. Such natural polyelectrolyte complexation and phase separation often occur at nonstoichiometric charge ratios with the incorporation of bioactive proteins, which is not studied as extensively as those complexations at stoichiometric ratios. In this work, we investigated how the addition of a crowding agent (polyethylene glycol, PEG) affected the complexation between chitosan (CS) and hyaluronic acid (HA), especially at nonstoichiometric ratios, and the encapsulation of enzyme (catalase, CAT) by the colloidal complexes. The crowded environment promoted colloidal phase separation at low charge ratios, forming complexes with increased colloidal and dissolution stability, which resulted in a smaller size and polydispersity (PDI). The binding isotherms revealed that the addition of PEG greatly enhanced the ion-pairing strength (with increased ion-pairing equilibrium constant K-a from 4.92 x 10(4) without PEG to 1.08 x 10(6) with 200 g/L PEG) and switched the coacervation from endothermic to exothermic, which explained the promoted complexation and phase separation. At the stoichiometric charge ratio, the enhanced CS-HA interaction in crowded media generated a more solid-like coacervate phase with a denser network, slower chain relaxation, and higher modulus. Moreover, both crowding and complex encapsulation enhanced the activity and catalytic efficiency of CAT, represented by a 2-fold increase in catalytic efficiency (K-cat/K-m) under 100 g/L PEG crowding and CS-HA complex encapsulation. This is likely due to the lower polarity in the microenvironment surrounding the enzyme molecules. By a systematic investigation of both nonstoichiometric and stoichiometric charge ratios under macromolecular crowding, this work provided new insights into the complexation between natural polyelectrolytes in a scenario closer to an intracellular environment.
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| GB/T 7714 | Ou, Xiatong , Tang, Ziyao , Ye, Yanqi et al. Macromolecular Crowding Effect on Chitosan-Hyaluronic Acid Complexation and the Activity of Encapsulated Catalase [J]. | BIOMACROMOLECULES , 2024 , 25 (6) : 3840-3849 . |
| MLA | Ou, Xiatong et al. "Macromolecular Crowding Effect on Chitosan-Hyaluronic Acid Complexation and the Activity of Encapsulated Catalase" . | BIOMACROMOLECULES 25 . 6 (2024) : 3840-3849 . |
| APA | Ou, Xiatong , Tang, Ziyao , Ye, Yanqi , Chen, Xiaochao , Huang, Yan . Macromolecular Crowding Effect on Chitosan-Hyaluronic Acid Complexation and the Activity of Encapsulated Catalase . | BIOMACROMOLECULES , 2024 , 25 (6) , 3840-3849 . |
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Insight is provided into the mechanism of peptide gelation, a self-assembled process of particular interest, due to its wide existence in natural events and broad applications in food and biomaterials field, but hard to control as minor alterations in the peptide sequence. Here, we design a family of pentapeptides and control their selfassembly behaviors, from micro- and nanoparticles to nanofibers and hydrogels. Furthermore, based on the thermodynamics, the gelation kinetics and the ionization status of terminal groups, the peptide assembly goes through five stages as varying the pH value and the assembly structures are highly dependent on the charging status of the terminal groups. The gelation rules of these peptides are further investigated. Besides, we also load hydrophobic functional molecule (curcumin) into different self-assembly nanostructures with high encapsulation efficiency and remarkable thermal and light protection on curcumin. These peptides assemblies with tunable structures, encapsulation ability and photothermal stabilization could become promising candidates for hydrophobic function factor delivery in food industry.
Keyword ：
Curcumin Curcumin Hydrogel Hydrogel Nanoparticle Nanoparticle Peptide Peptide Self-assembly Self-assembly
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| GB/T 7714 | Chen, Huimin , Zhang, Tianrui , Tian, Yongqi et al. Novel self-assembling peptide hydrogel with pH-tunable assembly microstructure, gel mechanics and the entrapment of curcumin [J]. | FOOD HYDROCOLLOIDS , 2022 , 124 . |
| MLA | Chen, Huimin et al. "Novel self-assembling peptide hydrogel with pH-tunable assembly microstructure, gel mechanics and the entrapment of curcumin" . | FOOD HYDROCOLLOIDS 124 (2022) . |
| APA | Chen, Huimin , Zhang, Tianrui , Tian, Yongqi , You, Lijun , Huang, Yan , Wang, Shaoyun . Novel self-assembling peptide hydrogel with pH-tunable assembly microstructure, gel mechanics and the entrapment of curcumin . | FOOD HYDROCOLLOIDS , 2022 , 124 . |
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The transition from soluble to colloidal polyelectrolyte complex normally occurs at a critical non-stoichiometric charge ratio. Here, it is demonstrated that the conventional batch mixing produces heterogeneous binding and complexation, which can easily mask this soluble-colloidal complex transition (sol-col transition) even for weakly binding polyelectrolytes like polyacrylic acid (PAA) and poly(diallyldimethylammonium chloride) (PDADMAC). When mixed efficiently using multi-inlet vortex mixer (MIVM), the sol-col transition occurs beyond a critical charge ratio (n-/n+) and the large colloidal complexes are formed through the aggregation of small primary complexes (as revealed by atomic force microscopy). Moreover, the sol-col transition occurs at a constant charge ratio below the overlapping concentration (c*) of the long host polyelectrolyte, but at lower charge ratios above c* due to chain entanglement. When adding NaCl to the solution, the sol-col transition charge ratio first decreases, then remained stable for a period, and finally increased and vanished at high ionic strength. When replacing NaCl with chaotropic salts, the sol-col transition occurs at lower charge ratios, while kosmotropes has little impact. The solvent quality and polymer hydrophobicity effects are also discussed. With the assistance of rapid mixing, this study provides a more reliable way of studying the sol-col transition of polyelectrolyte complexes.
Keyword ：
coacervate coacervate complexation complexation flash nanocomplexation flash nanocomplexation polyelectrolytes polyelectrolytes soluble complexes soluble complexes
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| GB/T 7714 | Gao, Jun , Zhan, Qiang-wei , Tang, Ziyao et al. The Critical Transition from Soluble Complexes to Colloidal Aggregates of Polyelectrolyte Complexes at Non-Stoichiometric Charge Ratios [J]. | MACROMOLECULAR RAPID COMMUNICATIONS , 2022 , 43 (7) . |
| MLA | Gao, Jun et al. "The Critical Transition from Soluble Complexes to Colloidal Aggregates of Polyelectrolyte Complexes at Non-Stoichiometric Charge Ratios" . | MACROMOLECULAR RAPID COMMUNICATIONS 43 . 7 (2022) . |
| APA | Gao, Jun , Zhan, Qiang-wei , Tang, Ziyao , Huang, Yan . The Critical Transition from Soluble Complexes to Colloidal Aggregates of Polyelectrolyte Complexes at Non-Stoichiometric Charge Ratios . | MACROMOLECULAR RAPID COMMUNICATIONS , 2022 , 43 (7) . |
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Due to the rapid reaction kinetics, the morphology of chitosan/tripolyphosphate nanoparticles was difficult to control. We mixed chitosan and tripolyphosphate through a multi-inlet vortex mixer at different mixing efficiency and characteristic mixing time (tau(mix)). Below a critical tau(mix), the aggregation of primary chitosan/TPP particles can be ceased. The corresponding aggregation time of primary particles (tau(agg)) was dependent on the ionic strength, the degree of deacetylation and molecular weight of chitosan. Moreover, the particle compactness was estimated from the hydrodynamic diameter and the turbidity using a model based on the Mie theory. Slow and nonhomogeneous mixing led to more compact nanoparticles, while rapid and homogeneous mixing pro-duced nanoparticles with a higher swelling ratio. Besides, the ionic strength and polymer concentration could affect the internal structure of nanoparticles. This study revealed the significance of mixing on the internal structure of chitosan/TPP nanoparticles, which could guide the preparation of other ionically-crosslinked polysaccharide colloids.
Keyword ：
Chitosan/tripolyphosphate nanoparticles Chitosan/tripolyphosphate nanoparticles Compactness Compactness Flash nano-complexation Flash nano-complexation Mixing kinetics Mixing kinetics
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| GB/T 7714 | Yuan, Yu , Gao, Jun , Zhai, Yuzhou et al. Mixing efficiency affects the morphology and compactness of chitosan/tripolyphosphate nanoparticles [J]. | CARBOHYDRATE POLYMERS , 2022 , 287 . |
| MLA | Yuan, Yu et al. "Mixing efficiency affects the morphology and compactness of chitosan/tripolyphosphate nanoparticles" . | CARBOHYDRATE POLYMERS 287 (2022) . |
| APA | Yuan, Yu , Gao, Jun , Zhai, Yuzhou , Li, Dongcui , Fu, Caili , Huang, Yan . Mixing efficiency affects the morphology and compactness of chitosan/tripolyphosphate nanoparticles . | CARBOHYDRATE POLYMERS , 2022 , 287 . |
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Achieving a high encapsulation efficiency and loading capacity of proteins in lecithin-based liposomes has always been a challenge. Here, we use Flash Nano-Precipitation (FNP) to produce liposomes and investigated the encapsulation of model protein (Bovine Serum Albumin, BSA). Through rapid turbulent mixing, we obtained liposomes with small size, low polydispersity, and good batch repeatability at a high production rate. We demonstrated that the bilayer of liposomes prepared solely using lecithin was defective, which led to the fusion, and increased size and polydispersity. When cholesterol was added to reach a lecithin-to-cholesterol molar ratio of 5:3, a compact bilayer formed to effectively inhibit liposome fusion. The encapsulation efficiency and loading capacity of BSA was as high as ti 68% and - 6% in lecithin-cholesterol liposome, respectively, far exceeding the values reported in the literature. Further study by Quartz Crystal Microbalance with Dissipation (QCM-D) revealed that the highly effective encapsulation was due to the rapid mutual adsorption between BSA and defective/curved lecithin double layers during the liposome formation. Such rapid mutual adsorption leads to the layer-by-layer assembly and formation of onion-like compact liposome structure as revealed by Cryo-TEM. This simple FNP method provides a scalable manufacturing approach for liposomes with efficient protein encapsulation. The revealed adsorption mechanism between protein and lecithin bilayers could also serve as a guide for similar studies.
Keyword ：
Bovine Serum Albumin Bovine Serum Albumin Dissipation Dissipation Flash Nano-Precipitation Flash Nano-Precipitation Liposome Liposome Quartz Crystal Microbalance with Quartz Crystal Microbalance with
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| GB/T 7714 | Zhan, Qiang-Wei , Gao, Jun , Li, Dongcui et al. High throughput onion-like liposome formation with efficient protein encapsulation under flash antisolvent mixing [J]. | JOURNAL OF COLLOID AND INTERFACE SCIENCE , 2022 , 618 : 185-195 . |
| MLA | Zhan, Qiang-Wei et al. "High throughput onion-like liposome formation with efficient protein encapsulation under flash antisolvent mixing" . | JOURNAL OF COLLOID AND INTERFACE SCIENCE 618 (2022) : 185-195 . |
| APA | Zhan, Qiang-Wei , Gao, Jun , Li, Dongcui , Huang, Yan . High throughput onion-like liposome formation with efficient protein encapsulation under flash antisolvent mixing . | JOURNAL OF COLLOID AND INTERFACE SCIENCE , 2022 , 618 , 185-195 . |
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Continuous and large-scale production of phospholipids stabilized emulsions and nanoparticles with controllable particle size and stability has always been a challenge. Here, we use flash nano-precipitation (FNP) to produce lecithin stabilized alpha-tocopheryl acetate (Vitamin E-acetate) particles at a production rate much higher than conventional batch mixing and thin-film hydration. The FNP produces particles with more homogeneous lecithin coverage comparing to conventional slow mixing. As determined by dynamic/electrophoretic light scattering, transmission electron microscope (TEM) and Quartz Crystal Microbalance with Dissipation (QCM-D), particles with low percent core are covered by thick multiple lecithin layers and aggregate easily with the coalescence of lecithin shell; particles with medium percent core content are covered with a thin layer of lecithin, which provides a high enough zeta-potential to stabilize the particle; for particles with percent core higher than 95%, the large spacing between lecithin molecules result in Ostwald ripening and aggregation. With a given composition, the particle size and stability could both be precisely predicted using the Smoluchowski's diffusion limited particle growth model and the Derjaguin-Landau, Verwey-Overbeek (DLVO) theory. This work demonstrates that the compositions affect the adsorption of phospholipid molecules at the particle surface, which further control particle size and stability.
Keyword ：
Flash nano-precipitation Flash nano-precipitation Lecithin Lecithin Nanoparticle Nanoparticle Quartz crystal microbalance with dissipation (QCM-D) Quartz crystal microbalance with dissipation (QCM-D)
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| GB/T 7714 | Zhan, Qiang-Wei , Huang, Yan . Continuous and large-scale fabrication of lecithin stabilized nanoparticles with predictable size and stability using flash nano-precipitation [J]. | LWT-FOOD SCIENCE AND TECHNOLOGY , 2021 , 139 . |
| MLA | Zhan, Qiang-Wei et al. "Continuous and large-scale fabrication of lecithin stabilized nanoparticles with predictable size and stability using flash nano-precipitation" . | LWT-FOOD SCIENCE AND TECHNOLOGY 139 (2021) . |
| APA | Zhan, Qiang-Wei , Huang, Yan . Continuous and large-scale fabrication of lecithin stabilized nanoparticles with predictable size and stability using flash nano-precipitation . | LWT-FOOD SCIENCE AND TECHNOLOGY , 2021 , 139 . |
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The infiltration of ions into hydrogel matrix could significantly affect the microstructure and macroscopic mechanics of the gels. Here, the Hofmeister effect of various salts on the whey protein isolate hydrogels with fine-stranded and particulate microstructures is investigated by soaking the preformed hydrogels in the sodium salts of different anions. The infiltration of kosmotropic anions yield stiffer hydrogels, whereas the chaotropic anions soften the hydrogels. The hydrogels with fine-stranded microstructures are more sensitive to the salts comparing to the particulate ones due to the microscopic phase transitions and enhanced hydrophobic interactions between polymer chains occurred in fine-stranded hydrogels. Besides, despite the significant difference in water binding ability of different salts, the water holding capacity of the salt-treated hydrogels was mainly determined by the gel stiffness instead of the salt types. Similar mechanical responses of BSA and egg white protein hydrogels to the Hofmeister series was also demonstrated, suggesting that the results shown here could potentially be generalized for other globular protein hydrogels.
Keyword ：
Hofmeister effect Hofmeister effect Mechanics Mechanics Microstructure Microstructure Protein hydrogel Protein hydrogel Water holding capacity Water holding capacity
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| GB/T 7714 | Lin, Junyan , Huang, Yan , Wang, Shaoyun . The Hofmeister effect on protein hydrogels with stranded and particulate microstructures [J]. | COLLOIDS AND SURFACES B-BIOINTERFACES , 2020 , 196 . |
| MLA | Lin, Junyan et al. "The Hofmeister effect on protein hydrogels with stranded and particulate microstructures" . | COLLOIDS AND SURFACES B-BIOINTERFACES 196 (2020) . |
| APA | Lin, Junyan , Huang, Yan , Wang, Shaoyun . The Hofmeister effect on protein hydrogels with stranded and particulate microstructures . | COLLOIDS AND SURFACES B-BIOINTERFACES , 2020 , 196 . |
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盐离子和pH影响蛋白质凝胶化过程,会导致凝胶微观结构及表观质构性质的显著差异。然而多数研究是通过直接向蛋白质溶液中添加无机盐或其他小分子的方法,这种方法在高盐浓度下会导致蛋白发生沉淀而无法形成具有良好网络结构的凝胶。本工作通过将乳清蛋白水凝胶浸泡在各种阴离子盐溶液中,研究了霍夫梅斯特效应对蛋白水凝胶的影响。结果发现水合性(kosmotropic)阴离子的加入使水凝胶强度增大,而离液盐(chaotropic)阴离子则使水凝胶强度降低。且与颗粒状凝胶相比,具有良好链状微观结构的水凝胶对盐的响应更为明显。通过进一步对水凝胶持水力进行表征,得出凝胶硬度和交联密度是影响凝胶持水力的主要因素,而浸泡盐的种...
Keyword ：
微观结构 微观结构 持水力 持水力 机械性能 机械性能 蛋白水凝胶 蛋白水凝胶 霍夫梅斯特效应 霍夫梅斯特效应
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| GB/T 7714 | 林珺琰 , 黄彦 . 霍夫梅斯特效应对链状和颗粒状蛋白质水凝胶的影响 [C] //中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第三卷） . 2019 . |
| MLA | 林珺琰 et al. "霍夫梅斯特效应对链状和颗粒状蛋白质水凝胶的影响" 中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第三卷） . (2019) . |
| APA | 林珺琰 , 黄彦 . 霍夫梅斯特效应对链状和颗粒状蛋白质水凝胶的影响 中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第三卷） . (2019) . |
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聚电解质与多价反离子可复合形成不同形态的聚电解质复合物。由于该复合反应迅速,导致其过程难以表征,复合物尺寸与形貌难以控制,且相似反离子时常展现出截然不同的复合过程。本课题使用壳聚糖为模型体系,通过添加单价盐的方法延缓了复合过程动力学,以及通过多通道快速混合法,排除传统混合方法中的局部过饱和和聚集,深入探究壳聚糖与蛋白质、多价盐(三聚磷酸钠)等多价反离子之间的复合过程[1, 2]。实验发现,壳聚糖与蛋白质之间的复合作用以及沉降浓度几乎完全由两者所带电荷决定。而壳聚糖与小分子多价磷酸盐之间的复合在均匀混合的情况下均经历了由可溶性复合物到直径15-30纳米的初级颗粒,再聚集为数百纳米的最终颗粒的过程...
Keyword ：
单价盐 单价盐 壳聚糖 壳聚糖 混合速率 混合速率 纳米颗粒 纳米颗粒 静电复合 静电复合
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| GB/T 7714 | 黄彦 , 袁育 . 壳聚糖与多价反离子复合过程调控与机理探究 [C] //中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第三卷） . 2019 . |
| MLA | 黄彦 et al. "壳聚糖与多价反离子复合过程调控与机理探究" 中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第三卷） . (2019) . |
| APA | 黄彦 , 袁育 . 壳聚糖与多价反离子复合过程调控与机理探究 中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第三卷） . (2019) . |
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聚电解质与反离子可复合形成不同形态的聚电解质复合物。由于该复合反应迅速,以及对混合过程控制不足,导致其过程难以表征,复合物形貌难以控制1-2。本课题使用壳聚糖/三聚磷酸钠为模型体系,通过快速混合的方法混合并与传统的逐滴滴加的方法进行比较,来探究导致不同形态的复合物的形成原因。实验发现,在低电荷比下,逐滴滴加过程中的不均匀混合和复合物的快速复合是导致不溶性复合物形成的主要原因。而使用快速混合的方法时,在混合均匀的条件下壳聚糖与三聚磷酸钠可以形成可溶性的复合物。通过对不同体系的验证,我们发现由Tsuchida3提出的"all or none"聚电解质复合机制是由于不均匀的混合以及聚电解质溶液逐滴滴...
Keyword ：
可溶性复合物 可溶性复合物 壳聚糖 壳聚糖 混合速率 混合速率 纳米颗粒 纳米颗粒 静电复合 静电复合
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| GB/T 7714 | 袁育 , 黄彦 . 快速混合法探究壳聚糖/三聚磷酸钠纳米颗粒生成过程与机理 [C] //中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第二卷） . 2019 . |
| MLA | 袁育 et al. "快速混合法探究壳聚糖/三聚磷酸钠纳米颗粒生成过程与机理" 中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第二卷） . (2019) . |
| APA | 袁育 , 黄彦 . 快速混合法探究壳聚糖/三聚磷酸钠纳米颗粒生成过程与机理 中国化学会第十七届全国胶体与界面化学学术会议论文（摘要）集（第二卷） . (2019) . |
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