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学者姓名：李锋
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Abstract ：
Biocatalytic technology has emerged as a powerful tool for synthesizing functional sugars with a wide range of physiological functions. D-lyxose isomerase is an important aldose-ketose isomerase used for synthesizing functional sugars such as L-ribose and D-mannose; however, at present, only a few D-lyxose isomerases have been studied. In this study, a metagenomic approach was employed to mine novel D-lyxose isomerases from the goat rumen microbiome. Eleven full-length D-lyxose isomerase genes were identified using sequence alignment and phylogenetic analysis. All 11 genes were classified as Group II D-lyxose isomerases and display low similarity to previously characterized D-lyxose isomerases. Five of the genes were selected for heterologous expression in Escherichia coli, and all were expressed successfully and had detectable enzyme activity. Further characterization revealed that optimum temperatures for the five enzymes range from 45 degrees C to 60 degrees C, and the optimum pH range is 7.0-8.0, with high relative residual activities under weakly acidic conditions. The recombinant enzymes have a broad substrate spectrum and are active against L-ribose, L-ribulose, D-fructose, and D-mannose, in addition to exhibiting the highest activity against D-lyxose. Among the five enzymes, GR-LI2 displays the highest activity toward L-ribulose, and GR-LI5 displays the highest activity against fructose, suggesting that these two enzymes have significant potential for L-ribose and D-mannose synthesis, respectively.
Keyword ：
D-lyxose isomerase D-lyxose isomerase D-mannose D-mannose Functional sugar Functional sugar L -ribose L -ribose Metagenomics Metagenomics Rare sugar Rare sugar Rumen Rumen
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| GB/T 7714 | Xu, Xin , Chen, Mingyi , Su, Yali et al. Identification and characterization of novel D-lyxose isomerases from the goat rumen metagenome [J]. | PROCESS BIOCHEMISTRY , 2025 , 149 : 213-221 . |
| MLA | Xu, Xin et al. "Identification and characterization of novel D-lyxose isomerases from the goat rumen metagenome" . | PROCESS BIOCHEMISTRY 149 (2025) : 213-221 . |
| APA | Xu, Xin , Chen, Mingyi , Su, Yali , Ding, Jiayun , Xie, Xiuye , Zhang, Xinyi et al. Identification and characterization of novel D-lyxose isomerases from the goat rumen metagenome . | PROCESS BIOCHEMISTRY , 2025 , 149 , 213-221 . |
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Traditional methods for aflatoxin B1 (AFB1) detection are complex, time-consuming, labor-intensive, and high cost. Moreover, they require sophisticated large-scale instrumentation, which limits their on-site rapid detection. Herein, phycocyanin fluorescent nanospheres based on fluorescence immunochromatographic assay were developed for quantitative detection of AFB1 at parts-per-billion (ppb) levels in foodstuffs. Phycocyanin and antiAFB1 monoclonal antibodies were coupled on the surface of latex nanospheres to amplify the fluorescence signal and improve the sensitivity. The fluorescence intensity was measured by a self-developed smartphone-based reading system. Under the optimal conditions, this approach achieved quantitative point-of-care detection of AFB1 within 25 min. The calibration curve for AFB1 was linear in the range of 0.2-48 ppb, and the limit of detection was 0.16 ppb. The practical applicability of the proposed approach was demonstrated by the determination of AFB1 in naturally contaminated samples, and the results were consistent with HPLC detection.
Keyword ：
Aflatoxin B1 Aflatoxin B1 Immunoassay Immunoassay On-site determination On-site determination Phycocyanin Phycocyanin Smartphone Smartphone
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| GB/T 7714 | Li, Shiqi , Zhong, Xiaoying , Xu, Yanan et al. Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs [J]. | FOOD CHEMISTRY , 2021 , 360 . |
| MLA | Li, Shiqi et al. "Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs" . | FOOD CHEMISTRY 360 (2021) . |
| APA | Li, Shiqi , Zhong, Xiaoying , Xu, Yanan , Zheng, Yunquan , Shi, Xianai , Li, Feng et al. Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs . | FOOD CHEMISTRY , 2021 , 360 . |
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In this work, the acute and subchronic toxicities of desaminotyrosine (DAT) by oral administration in SD rats and its effects on the intestinal microflora were investigated. The acute toxicity test showed that DAT is a low-toxic substance with a LD50 of 3129 mg/kg. The subchronic toxicity test showed that DAT has no toxicity at a low dose (125 mg/kg/day). However, DAT exhibited obvious toxicities to food intake, liver, kidney, and lung at higher dose (250 mg/kg/day and 500 mg/kg/day). DAT inhibited the food intake of rats in a dose-dependent manner. Serum biochemical analysis showed that DAT can increase the serum glucose level of rats. Fecal microbiota analysis showed that DAT treatment can significantly change the intestinal microflora of rats, the dose of 125 mg/kg/day has the most significant effect on the diversity of intestinal microbiota. In daily application, the side effects caused by DAT might be gastrointestinal irritation, weight loss, liver or kidney injury, and blood sugar elevation. Based on our study, the no-observed-adverse-effect level (NOAEL) of DAT is 125 mg/kg BW/day for rats.
Keyword ：
Acute toxicity Acute toxicity Desaminotyrosine Desaminotyrosine Intestinal microflora Intestinal microflora Metabolites of flavonoid Metabolites of flavonoid Subachronic toxicity Subachronic toxicity
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| GB/T 7714 | Li, Feng , Wang, Liping , Cai, Yilei et al. Safety assessment of desaminotyrosine: Acute, subchronic oral toxicity, and its effects on intestinal microbiota in rats [J]. | TOXICOLOGY AND APPLIED PHARMACOLOGY , 2021 , 417 . |
| MLA | Li, Feng et al. "Safety assessment of desaminotyrosine: Acute, subchronic oral toxicity, and its effects on intestinal microbiota in rats" . | TOXICOLOGY AND APPLIED PHARMACOLOGY 417 (2021) . |
| APA | Li, Feng , Wang, Liping , Cai, Yilei , Luo, Yihuo , Shi, Xianai . Safety assessment of desaminotyrosine: Acute, subchronic oral toxicity, and its effects on intestinal microbiota in rats . | TOXICOLOGY AND APPLIED PHARMACOLOGY , 2021 , 417 . |
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将源自鲨鱼软骨的高纯度硫酸软骨素和胶原蛋白以质量比2∶1配伍,得到鲨鱼硫酸软骨素胶原蛋白复方(SCC),并考察了该复方对骨关节炎(OA)的改善作用。在实验的第0、2、4、6天向小鼠膝关节腔定量注射Ⅱ型胶原蛋白酶,构建骨关节炎小鼠实验模型。同时每天分别以300、150 mg·kg~(-1)剂量灌胃小鼠给予SCC干预,持续4周。之后,取关节组织,测定肿胀程度,病理切片染色分析软骨损伤情况;通过酶联免疫吸附法(ELISA)检测血清IL-1β和IL-4表达水平。关节病理切片H&E染色结果显示,SCC 300 mg·kg~(-1)剂量对软骨细胞的修复优于150 mg·kg~(-1)剂量;Masson和天...
Keyword ：
硫酸软骨素 硫酸软骨素 胶原蛋白 胶原蛋白 骨关节炎 骨关节炎 鲨鱼软骨 鲨鱼软骨
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| GB/T 7714 | 颜晨燕 , 王梨萍 , 屈鑫 et al. 鲨鱼硫酸软骨素及胶原蛋白复方改善小鼠骨关节炎作用研究 [J]. | 海洋渔业 , 2020 , 42 (06) : 740-750 . |
| MLA | 颜晨燕 et al. "鲨鱼硫酸软骨素及胶原蛋白复方改善小鼠骨关节炎作用研究" . | 海洋渔业 42 . 06 (2020) : 740-750 . |
| APA | 颜晨燕 , 王梨萍 , 屈鑫 , 李锋 , 石贤爱 . 鲨鱼硫酸软骨素及胶原蛋白复方改善小鼠骨关节炎作用研究 . | 海洋渔业 , 2020 , 42 (06) , 740-750 . |
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Background: It has been demonstrated in some studies that triterpenoid acid extract fromEriobotrya japonica leaf is beneficial to prevent hyperlipidemia or insulin resistance. However, the effect of triterpenoid acids in Eriobotrya japonica leaf on a series of typical symptoms of metabolic syndrome (MetS) has been rarely studied systemati-cally. Therefore, the present study aims to systematically evaluate the effect of Eriobotrya japonica leaf triterpenoid acids (ELTA) on MetS and explore its potential mechanism. Methods: ELTA (HPLC purity 95.2 %) was prepared and administered orally (200 mg/kg) to C57BL/6 J mice fed with a high-fat diet (HFD) for 12 weeks. Pioglitazone (30 mg/kg) was used as a positive control drug. Food intake, body weight, total lipid in feces, lipid profiles, inflammatory factors in serum, hepatic glutathione, and lipid peroxide were measured. Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were performed to evaluate insulin sensitivity. RT-qPCR and molecular docking were performed to explore the potential mechanism. Results: ELTA administration reduced body weight gain, relative liver weight, and relative visceral adipose weight. The levels of serum total cholesterol, triglycerides, low-density lipoprotein cholesterol, hepatic total cholesterol, and hepatic triglycerides were also reduced. ELTA reduced the area under curve (AUC) of blood glucose curves in OGTT and ITT. Relative mRNA level analysis of genes related to MetS showed that ELTA can effectively increase the transcriptional levels of Nrf2, HO-1, PPAR-gamma, GluT2, GK, FXR, while effectively decrease those of PTP1B, p65, TNF-alpha, IL-6, SREBP, 11 beta HSD-1. Molecular docking showed that the ligands in ELTA can bind to 11 beta HSD-1, GK, PPAR-gamma, and JNK, the important targets involved in MetS. Conclusions: ELTA can effectively alleviate visceral central obesity, insulin resistance, dyslipidemia, oxidative stress, and inflammation of HFD-induced MetS in C57BL/6 J mice. This is possibly achieved by acting on 11 beta HSD-1, GK, PPAR-gamma, and JNK.
Keyword ：
Dyslipidemia Dyslipidemia Eriobotrya japonica leaf triterpenoid acids Eriobotrya japonica leaf triterpenoid acids High-fat diet High-fat diet Insulin resistance Insulin resistance Metabolic syndrome Metabolic syndrome Molecular docking Molecular docking
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| GB/T 7714 | Li, Feng , Li, Yijia , Li, Qingxian et al. Eriobotrya japonica leaf triterpenoid acids ameliorate metabolic syndrome in C57BL/6J mice fed with high-fat diet [J]. | BIOMEDICINE & PHARMACOTHERAPY , 2020 , 132 . |
| MLA | Li, Feng et al. "Eriobotrya japonica leaf triterpenoid acids ameliorate metabolic syndrome in C57BL/6J mice fed with high-fat diet" . | BIOMEDICINE & PHARMACOTHERAPY 132 (2020) . |
| APA | Li, Feng , Li, Yijia , Li, Qingxian , Shi, Xianai . Eriobotrya japonica leaf triterpenoid acids ameliorate metabolic syndrome in C57BL/6J mice fed with high-fat diet . | BIOMEDICINE & PHARMACOTHERAPY , 2020 , 132 . |
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Acute full-thickness wounds require amore extended healing period, thus increasing the risk of infection. Severe infection frequently resulted in wound ulceration, necrosis, and even life-threatening complications. Here, a hybrid hydrogel comprising aminated collagen (AC), oxidized sodium alginate (OSA), and antimicrobial peptides (polymyxin B sulfate and bacitracin) was developed to enhance full-thickness wound healing. The AC with low immunogenicity and high biocompatibility was made from marine fish scales, which are eco-friendly, low-cost, and sustainable. The cross-linked hydrogel was formed by a Schiff base reaction without any catalysts and additional procedures. As expected, the presented hybrid hydrogel can effectively against E. coli and S. aureus, as well as promote cell growth and angiogenesis in vitro. In addition, the hydrogel can promote full-thickness wound healing in a rat model through accelerating reepithelialization, collagen deposition, and angiogenesis. Our work demonstrated that the hybrid hydrogel has promising applications in the field of wound healing, which would prompt the utilization of marine fish resources during food processing. (C) 2020 Elsevier B.V. All rights reserved.
Keyword ：
Antimicrobial peptide Antimicrobial peptide Fish scale Fish scale Hydrogel Hydrogel Sodium alginate Sodium alginate Wound dressing Wound dressing
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| GB/T 7714 | Feng, Xiaolian , Zhang, Xiaofang , Li, Shiqi et al. Preparation of aminated fish scale collagen and oxidized sodium alginate hybrid hydrogel for enhanced full-thickness wound healing [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2020 , 164 : 626-637 . |
| MLA | Feng, Xiaolian et al. "Preparation of aminated fish scale collagen and oxidized sodium alginate hybrid hydrogel for enhanced full-thickness wound healing" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 164 (2020) : 626-637 . |
| APA | Feng, Xiaolian , Zhang, Xiaofang , Li, Shiqi , Zheng, Yunquan , Shi, Xianai , Li, Feng et al. Preparation of aminated fish scale collagen and oxidized sodium alginate hybrid hydrogel for enhanced full-thickness wound healing . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2020 , 164 , 626-637 . |
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In the human genome, single-nucleotide polymorphisms (SNPs) can often lead to the activation of oncogenes and have a close association with tumor development or progression. Specific identification of SNPs and quantitative analysis of oncogenes could provide insight into the basis for early diagnosis and risk assessment of malignancy, as well as personalized medicine. Herein, we proposed a new biosensing platform to screen SNPs through the ligation/polymerization-mediated signaling of a split molecular beacon (SMB) where target-recycling benefits can be achieved. The SMB consists of two fragments, one of which contains a fluorophore group and the other was modified with a quencher group. In the absence of target DNA, the two fragments hybridize with each other and the fluorescence is quenched. After exposure to a wild-type target, they can be opened and ligated together by the ligase using the target as the template. Moreover, the primer is able to bind to target hybridization-released binding sites and initiate strand-displacement amplification (SDA), generating an enhanced signal. By combining the specificity of nick ligation with the signal amplification of target recycling, the human K-ras oncogene as a model target can be detected down to 8 pM. Moreover, a wide dynamic range (more than 3 orders of magnitude) and excellent ability to discriminate single-base mutations are achieved. Due to the homogeneous reaction and simple experimental steps, the target assay is greatly cost-efficient and readily automated. The desirable features and advantages make the proposed genotyping strategy a promising platform for genetic analysis and molecular diagnostics.
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| GB/T 7714 | Li, Min , Jiang, Min , Yang, Shu-Lin et al. A split molecular beacon for specific identification of cancer-related single nucleotide polymorphism [J]. | ANALYTICAL METHODS , 2019 , 11 (25) : 3252-3259 . |
| MLA | Li, Min et al. "A split molecular beacon for specific identification of cancer-related single nucleotide polymorphism" . | ANALYTICAL METHODS 11 . 25 (2019) : 3252-3259 . |
| APA | Li, Min , Jiang, Min , Yang, Shu-Lin , Guo, Fei-Xia , Wu, Bi-Ting , Zhang, Meng-Yi et al. A split molecular beacon for specific identification of cancer-related single nucleotide polymorphism . | ANALYTICAL METHODS , 2019 , 11 (25) , 3252-3259 . |
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Wound dressings with long-term antimicrobial activity are highly desired for treatment of chronic wound infections. Herein, the sustained antimicrobial wound dressings were developed by using antibiotic agents, ciprofloxacin HCL (CIP) and gentamicin sulfate (GS), covalent bonding to natural polymer matrix composites, carboxymethyl chitosan (CMC) and collagen (COL). By amide bond formation between antibiotic agents and polymer chains, two antimicrobial wound dressings CMC-COL-CIP and CMC-COL-GS were prepared. The presented wound dressings exhibited high water absorption capacity, excellent water vapor transmission rate (WVTR), appropriate mechanical properties, and impressive stability. Cytocompatibility of the dressings was demonstrated by in vitro human skin fibroblast (HSF) cells culture study. The results of in vitro and in vivo studies indicated that the two antimicrobial wound dressings have effective antimicrobial activity and prolonged antimicrobial period. Furthermore, the antimicrobial dressings could promote the wound healing, reepithelialization, collagen deposition, and angiogenesis. It also displays superiority wound healing effects compared to commercially available silver-based dressings (Aguacel Ag). This work indicates that the prepared antimicrobial wound dressings have great potential application in chronic wound healing, such as severe wound cure and diabetic foot ulcers. (C) 2018 Elsevier B.V. All rights reserved.
Keyword ：
Antimicrobial activity Antimicrobial activity Carboxymethyl chitosan Carboxymethyl chitosan Collagen Collagen Wound dressings Wound dressings Wound infections Wound infections
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| GB/T 7714 | Hu, Shengxue , Cai, Xianqun , Qu, Xin et al. Preparation of biocompatible wound dressings with long-term antimicrobial activity through covalent bonding of antibiotic agents to natural polymers [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2019 , 123 : 1320-1330 . |
| MLA | Hu, Shengxue et al. "Preparation of biocompatible wound dressings with long-term antimicrobial activity through covalent bonding of antibiotic agents to natural polymers" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 123 (2019) : 1320-1330 . |
| APA | Hu, Shengxue , Cai, Xianqun , Qu, Xin , Yu, Bangrui , Yan, Chenyan , Yang, Jianmin et al. Preparation of biocompatible wound dressings with long-term antimicrobial activity through covalent bonding of antibiotic agents to natural polymers . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2019 , 123 , 1320-1330 . |
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Herein, an autonomous table-tennis-motion-type DNA machine (ATT DNA machine) was proposed to integrate target recycling amplification (TRA) and triple strand-displacement amplification (T-SDA) into one signaling system for the efficient amplification detection of p53 gene. Firstly, X-shaped DNA duplex probe (XSP) was formed via the simple annealing of Probe A and Probe B. Upon addition of p53, the hybridization of p53 with Probe A results in the peeling of Probe B from XSP. Then, the primer binds to the Probe B to trigger a circular strand-displacement amplification, during which new primers are generated. Moreover, the newly-produced primers can in turn activate the target recycling amplification and triple strand-displacement amplification via bouncing back and forth between different Probe As and Probe Bs in a manner analogous to the table tennis motion. This newly-developed DNA machine can be used to detect p53 down to 0.5 pM with an excellent specificity, offering a 100-fold enhanced sensitivity compared to a non-amplification one-to-one hybridization based system. Moreover, this DNA machine is able to be applied for target analysis in complex environments. Additionally, the potential for practical applications is also verified, demonstrating a promising alternative tool for early diagnosis of cancers.
Keyword ：
ATT DNA machine ATT DNA machine p53 gene p53 gene Target recycling amplification (TRA) Target recycling amplification (TRA) Triple strand-displacement amplification (TSDA) Triple strand-displacement amplification (TSDA) X-shaped DNA duplex probe (XSP) X-shaped DNA duplex probe (XSP)
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| GB/T 7714 | Xu, Huo , Zhang, Yafeng , Zhang, Shuxin et al. Autonomous table-tennis-motion-type DNA machine for the efficient amplification detection of cancer related gene [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2019 , 281 : 1016-1022 . |
| MLA | Xu, Huo et al. "Autonomous table-tennis-motion-type DNA machine for the efficient amplification detection of cancer related gene" . | SENSORS AND ACTUATORS B-CHEMICAL 281 (2019) : 1016-1022 . |
| APA | Xu, Huo , Zhang, Yafeng , Zhang, Shuxin , Ouyang, Changhe , Li, Feng , Yu, Suhong et al. Autonomous table-tennis-motion-type DNA machine for the efficient amplification detection of cancer related gene . | SENSORS AND ACTUATORS B-CHEMICAL , 2019 , 281 , 1016-1022 . |
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The purpose of the current study was to construct a -cyclodextrin drug carrier for rubropunctatin to improve its water solubility and light stability for future cytotoxicity studies. The inclusion complexation behavior of rubropunctatin with -cyclodextrin was investigated using FESEM, FT-IR and XRD. A molecular docking study was performed to elucidate the most probable inclusion structure. The inclusion complex could be completely dispersed in water and had a small size of 121.87 +/- 2.13 nm (n = 3), a good PDI (0.320 +/- 0.017), and an acceptable potential value of -27.7 +/- 0.32 mV (n = 3). Furthermore, the stability of the rubropunctatin in water under light irradiation was found to be greatly enhanced after being encapsulated in cyclodextrin, and it exhibited a retention rate of over 70% vs. 10.17%. In addition, the cytotoxicity of the inclusion complex was evaluated by MTT assay and Annexin V-FITC/PI detection using cervical adenocarcinoma HeLa cells. The results showed that the inclusion complex had comparable toxicity compared to rubropunctatin solubilized with 0.4% DMSO. More importantly, the formation of the inclusion complex contributed greatly to the intensification of the bioavailability of rubropunctatin because the use of organic solvent was avoided.
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| GB/T 7714 | Ren, Zhenzhen , Xu, Yanan , Lu, Zhenxin et al. Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier [J]. | RSC ADVANCES , 2019 , 9 (20) : 11396-11405 . |
| MLA | Ren, Zhenzhen et al. "Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier" . | RSC ADVANCES 9 . 20 (2019) : 11396-11405 . |
| APA | Ren, Zhenzhen , Xu, Yanan , Lu, Zhenxin , Wang, Zhenzhen , Chen, Chengqun , Guo, Yanghao et al. Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier . | RSC ADVANCES , 2019 , 9 (20) , 11396-11405 . |
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