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学者姓名：郭养浩
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Abstract ：
The purpose of the current study was to construct a -cyclodextrin drug carrier for rubropunctatin to improve its water solubility and light stability for future cytotoxicity studies. The inclusion complexation behavior of rubropunctatin with -cyclodextrin was investigated using FESEM, FT-IR and XRD. A molecular docking study was performed to elucidate the most probable inclusion structure. The inclusion complex could be completely dispersed in water and had a small size of 121.87 +/- 2.13 nm (n = 3), a good PDI (0.320 +/- 0.017), and an acceptable potential value of -27.7 +/- 0.32 mV (n = 3). Furthermore, the stability of the rubropunctatin in water under light irradiation was found to be greatly enhanced after being encapsulated in cyclodextrin, and it exhibited a retention rate of over 70% vs. 10.17%. In addition, the cytotoxicity of the inclusion complex was evaluated by MTT assay and Annexin V-FITC/PI detection using cervical adenocarcinoma HeLa cells. The results showed that the inclusion complex had comparable toxicity compared to rubropunctatin solubilized with 0.4% DMSO. More importantly, the formation of the inclusion complex contributed greatly to the intensification of the bioavailability of rubropunctatin because the use of organic solvent was avoided.
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| GB/T 7714 | Ren, Zhenzhen , Xu, Yanan , Lu, Zhenxin et al. Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier [J]. | RSC ADVANCES , 2019 , 9 (20) : 11396-11405 . |
| MLA | Ren, Zhenzhen et al. "Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier" . | RSC ADVANCES 9 . 20 (2019) : 11396-11405 . |
| APA | Ren, Zhenzhen , Xu, Yanan , Lu, Zhenxin , Wang, Zhenzhen , Chen, Chengqun , Guo, Yanghao et al. Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier . | RSC ADVANCES , 2019 , 9 (20) , 11396-11405 . |
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Phycocyanin (PC) is a natural fluorescent protein which is widely used in medical diagnosis, biology, biotechnology, foods, and medicine. However, the disadvantages in traditional purification methods, such as complicated preparation process, low yield, inadequate quantity, and high price, limit its range of application. In this study, we established a rapid purification method suitable for large-scale production to prepare PC from Arthrospira platensis. The membrane separation and two-stage ammonium sulfate precipitation yielded PC with a purity ratio (A(620nm)/A(280nm)) of 3.65. This purification method of PC proved to be efficient and cost-effective compared with previously reported methods. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that the molecular weight of alpha and beta subunits of PC was 16.6 and 21.2 kDa. The main molecular weight of intact PC determined through high-performance size exclusion chromatography (HPSEC) was around 91 kDa, indicating that the PC from A. platensis existed mainly in the form of (alpha beta)(3) at pH 7.0. The purified PC fluorescent probe was compatible for light-emitting diode (LED)-charge-coupled device (CCD) fluorescence density strip qualitative detection system. A relatively sensitive novel fluorescent test method was developed for the quantitative detection of PC showing a linear response to PC within the range of 0.07-3.64 mu M, and with a limit of detection of 0.034 mu M. This method could provide expedient quantitative information and showed great potential for rapid detection in environmental and food safety research.
Keyword ：
Fluorescent protein Fluorescent protein Large-scale purification Large-scale purification Rapid detection Rapid detection
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| GB/T 7714 | Zheng, Yunquan , Mo, Liuda , Zhang, Wenyi et al. Phycocyanin fluorescent probe from Arthrospira platensis: preparation and application in LED-CCD fluorescence density strip qualitative detection system [J]. | JOURNAL OF APPLIED PHYCOLOGY , 2019 , 31 (2) : 1107-1115 . |
| MLA | Zheng, Yunquan et al. "Phycocyanin fluorescent probe from Arthrospira platensis: preparation and application in LED-CCD fluorescence density strip qualitative detection system" . | JOURNAL OF APPLIED PHYCOLOGY 31 . 2 (2019) : 1107-1115 . |
| APA | Zheng, Yunquan , Mo, Liuda , Zhang, Wenyi , Duan, Yunjian , Huang, Jiandong , Chen, Chengqun et al. Phycocyanin fluorescent probe from Arthrospira platensis: preparation and application in LED-CCD fluorescence density strip qualitative detection system . | JOURNAL OF APPLIED PHYCOLOGY , 2019 , 31 (2) , 1107-1115 . |
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Alzheimer's disease (AD) is an extremely complex disease, characterized by several pathological features including oxidative stress and amyloid-beta (A beta) aggregation. Blockage of A beta-induced injury has emerged as a potential therapeutic approach for AD. Our previous efforts resulted in the discovery of Monascus pigment rubropunctatin derivative FZU-H with potential neuroprotective effects. This novel lead compound significantly diminishes toxicity induced by A beta(1-42) in Neuro-2A cells. Our further mechanism investigation revealed that FZU-H inhibited A beta(1-42)-induced caspase-3 protein activation and the loss of mitochondrial membrane potential. In addition, treatment of FZU-H was proven to attenuate A beta(1-42)-induced cell redox imbalance and Tau hyperphosphorylation which caused by okadaic acid in Neuro-2A cells. These results indicated that FZU-H shows promising neuroprotective effects for AD.
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| GB/T 7714 | Zheng, Yunquan , Pan, Qisheng , Mo, Liuda et al. Monascus pigment rubropunctatin derivative FZU-H reduces A beta(1-42)-induced neurotoxicity in Neuro-2A cells [J]. | RSC ADVANCES , 2018 , 8 (31) : 17389-17398 . |
| MLA | Zheng, Yunquan et al. "Monascus pigment rubropunctatin derivative FZU-H reduces A beta(1-42)-induced neurotoxicity in Neuro-2A cells" . | RSC ADVANCES 8 . 31 (2018) : 17389-17398 . |
| APA | Zheng, Yunquan , Pan, Qisheng , Mo, Liuda , Zhang, Wenyi , Duan, Yunjian , Chen, Chengqun et al. Monascus pigment rubropunctatin derivative FZU-H reduces A beta(1-42)-induced neurotoxicity in Neuro-2A cells . | RSC ADVANCES , 2018 , 8 (31) , 17389-17398 . |
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In an aqueous buffer system, serious reverse and side reactions were found in the asymmetric reduction of 2-octanone with Saccharomyces cerevisiae. However, some nonionic surfactants added to the aqueous buffer system improved the bioreduction process by decreasing the reverse and side reaction rates in addition to effectively increasing the average positive reaction rate. Further, a shorter carbon chain length of hydrophilic or hydrophobic moieties in surfactants resulted in a higher yield of (S)-2-octanol. The alkylphenol ethoxylate surfactants had a less influence than polyoxyethylenesorbitan trialiphatic surfactants on the product e.e. It suggested that the product e.e. resulting from the change of carbon chain length of the hydrophobic moieties varied markedly compared with the change of carbon chain length of the hydrophilic moiety. Emulsifier OP-10 and Tween 20 markedly enhanced the yield and product e.e. at the concentration of 0.4 mmol L-1 with a yield of 73.3 and 93.2%, and the product e.e. of 99.2 and 99.3%, respectively, at the reaction time of 96 h.
Keyword ：
2-Octanone 2-Octanone Asymmetric reduction Asymmetric reduction Nonionic surfactant Nonionic surfactant Saccharomyces cerevisiae Saccharomyces cerevisiae
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| GB/T 7714 | Zheng, Yunquan , Li, Liangbin , Shi, Xianai et al. Nonionic surfactants and their effects on asymmetric reduction of 2-octanone with Saccharomyces cerevisiae [J]. | AMB EXPRESS , 2018 , 8 . |
| MLA | Zheng, Yunquan et al. "Nonionic surfactants and their effects on asymmetric reduction of 2-octanone with Saccharomyces cerevisiae" . | AMB EXPRESS 8 (2018) . |
| APA | Zheng, Yunquan , Li, Liangbin , Shi, Xianai , Huang, Zhijian , Li, Feng , Yang, Jianmin et al. Nonionic surfactants and their effects on asymmetric reduction of 2-octanone with Saccharomyces cerevisiae . | AMB EXPRESS , 2018 , 8 . |
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从枇杷叶中提取分离制备科罗索酸,采用体外Cu~(2+)诱导低密度脂蛋白(low density lipoprotein,LDL)氧化损伤的体外化学反应模型及2,2’-偶氮二(2-甲基丙基咪)二盐酸盐(2,2’-azobis-2-methyl-propanimidamide dihydrochloride,AAPH)诱导人主动脉内皮细胞(human aortic endothelial cells,HAECs)氧化损伤的细胞模型,考察枇杷叶科罗索酸对LDL氧化过程的抑制作用及对动脉血管内皮细胞氧化损伤的保护作用。体外实验结果表明,枇杷叶科罗索酸在体外10~100μmol/L剂量范围内能有效延长L...
Keyword ：
低密度脂蛋白 低密度脂蛋白 枇杷叶 枇杷叶 科罗索酸 科罗索酸 脂质过氧化 脂质过氧化 血管内皮细胞 血管内皮细胞
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| GB/T 7714 | 李锋 , 李义嘉 , 李清仙 et al. 枇杷叶科罗索酸抑制人低密度脂蛋白氧化修饰及保护血管内皮细胞氧化损伤作用 [J]. | 食品科学 , 2017 , 38 (15) : 215-220 . |
| MLA | 李锋 et al. "枇杷叶科罗索酸抑制人低密度脂蛋白氧化修饰及保护血管内皮细胞氧化损伤作用" . | 食品科学 38 . 15 (2017) : 215-220 . |
| APA | 李锋 , 李义嘉 , 李清仙 , 郭养浩 , 石贤爱 . 枇杷叶科罗索酸抑制人低密度脂蛋白氧化修饰及保护血管内皮细胞氧化损伤作用 . | 食品科学 , 2017 , 38 (15) , 215-220 . |
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从坛紫菜中提取分离制备多酚组分,通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、抑制β-胡萝卜素褪色能力和铁还原能力分析法评价坛紫菜多酚的体外抗氧化作用;以紫外线B(ultraviolet radiation B,UVB)致人表皮成纤维细胞(human skin fibroblast cells,HSFs)损伤模型评价坛紫菜多酚抑制紫外损伤表皮作用,考察其用于防护紫外损伤领域的可行性。体外实验结果显示,坛紫菜多酚具有较强的抗氧化作用,其DPPH自由基清除能力与同等质量浓度的二丁基羟基甲苯(butylated hydrox...
Keyword ：
坛紫菜多酚 坛紫菜多酚 抗氧化 抗氧化 氧化应激 氧化应激 紫外线B 紫外线B
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| GB/T 7714 | 李锋 , 李清仙 , 程志远 et al. 坛紫菜多酚抗氧化及抑制UVB致HSF细胞氧化损伤作用 [J]. | 食品科学 , 2017 , 38 (17) : 190-197 . |
| MLA | 李锋 et al. "坛紫菜多酚抗氧化及抑制UVB致HSF细胞氧化损伤作用" . | 食品科学 38 . 17 (2017) : 190-197 . |
| APA | 李锋 , 李清仙 , 程志远 , 郭养浩 , 石贤爱 . 坛紫菜多酚抗氧化及抑制UVB致HSF细胞氧化损伤作用 . | 食品科学 , 2017 , 38 (17) , 190-197 . |
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本发明提供了一种提取螺旋藻中活性物质的方法，采用螺旋藻为原料，依次采用不同极性有机溶剂提取，分步提取β‑胡萝卜素、叶黄素、叶绿素和藻蓝蛋白。目前螺旋藻主要用于提取藻蓝蛋白，本发明能充分利用螺旋藻，延伸了螺旋藻深加工的产业链，将产生更高的经济效益。
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| GB/T 7714 | 郑允权 , 李继雨 , 莫柳达 et al. 一种提取螺旋藻中活性物质的方法 : CN201710047019.5[P]. | 2017/1/22 . |
| MLA | 郑允权 et al. "一种提取螺旋藻中活性物质的方法" : CN201710047019.5. | 2017/1/22 . |
| APA | 郑允权 , 李继雨 , 莫柳达 , 黄剑东 , 郭养浩 . 一种提取螺旋藻中活性物质的方法 : CN201710047019.5. | 2017/1/22 . |
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Impaired endothelial functions are closely associated with many chronic vascular-related diseases. Maslinic acid (MA), a natural pentacyclic triterpene compound, has received more and more attentions in recent years due to a variety of bioactivities. In the present study, we investigated the effect of MA on impaired endothelial functions in human aortic endothelial cells (HAEC) induced by high glucose treatment and discussed its possible mechanism. We showed that MA decreased the ROS level, inhibited the inflammatory cytokines expression, facilitated insulin-mediated IRS-1/PI3K/Akt/eNOS signaling and suppressed the cellular apoptosis ratio induced by high glucose. The molecular docking results showed that MA may regulate multiple targets to improve the endothelial dysfunction in HAECs exposed to high glucose. These results revealed potential application of MA in improving endothelia dysfunction.
Keyword ：
Apoptosis Apoptosis HAEC HAEC High glucose High glucose Impaired endothelial function Impaired endothelial function Maslinic acid Maslinic acid Molecular docking Molecular docking
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| GB/T 7714 | Li, Feng , Li, Qingxian , Shi, Xianai et al. Maslinic acid inhibits impairment of endothelial functions induced by high glucose in HAEC cells through improving insulin signaling and oxidative stress [J]. | BIOMEDICINE & PHARMACOTHERAPY , 2017 , 95 : 904-913 . |
| MLA | Li, Feng et al. "Maslinic acid inhibits impairment of endothelial functions induced by high glucose in HAEC cells through improving insulin signaling and oxidative stress" . | BIOMEDICINE & PHARMACOTHERAPY 95 (2017) : 904-913 . |
| APA | Li, Feng , Li, Qingxian , Shi, Xianai , Guo, Yanghao . Maslinic acid inhibits impairment of endothelial functions induced by high glucose in HAEC cells through improving insulin signaling and oxidative stress . | BIOMEDICINE & PHARMACOTHERAPY , 2017 , 95 , 904-913 . |
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The interest focusing on Eriobotrya japonica leaf triterpene acid (ELTA) has increased recently because of its beneficial effects on health. However, there has been a lack of experimental data on its toxicity. The present study therefore was conducted to evaluate its toxicity in ICR mice. The results showed that ELTA produced neither mortality nor toxicity of the main organs in ICR male and female mice in both acute (0.30, 0.65, 1.39, and 3.00 g center dot kg(-1) body weight) and subacute (150, 300, and 600mg center dot kg(-1) BW) 28-day toxicity studies. Because of lacking apparently adverse effects found in the hematology, clinical biochemistry, and histopathology evaluation, results of the present study together with the beneficial effects make ELTA as a promising dietary supplement and indicated that ELTA administered orally might have a large safety margin for human.
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| GB/T 7714 | Li, Feng , Li, Yijia , Li, Qingxian et al. Acute and Subacute Oral Toxicity Evaluation of Eriobotrya japonica Leaf Triterpene Acids in ICR Mice [J]. | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE , 2017 , 2017 . |
| MLA | Li, Feng et al. "Acute and Subacute Oral Toxicity Evaluation of Eriobotrya japonica Leaf Triterpene Acids in ICR Mice" . | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2017 (2017) . |
| APA | Li, Feng , Li, Yijia , Li, Qingxian , Shi, Xianai , Guo, Yanghao . Acute and Subacute Oral Toxicity Evaluation of Eriobotrya japonica Leaf Triterpene Acids in ICR Mice . | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE , 2017 , 2017 . |
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In the present study, corosolic acid (CA) was extracted and separated from Folium Eriobotryae. A Cu2+-induced low density lipoprotein (LDL) oxidation model was employed to evaluate the inhibitory effect of CA on LDL oxidation in vitro, and a human aortic endothelial cell (HAEC) model of oxidative damage induced by (2,2'-azobis-2-methylpropanimidamide dihydrochloride, AAPH) was employed to evaluate the protective effect of CA on oxidative damage. In the dose range of 10-100 μmol/L, CA effectively extended the lag time of the Cu2+-induced LDL oxidation process, reduced the area under the oxidation kinetic curve (AUC) and inhibited the generation of lipid peroxide indicating that CA could effectively inhibit LDL oxidation induced by Cu2+. In the cellular experiment at the dose range of 2-10 μmol/L, CA effectively reduced LDH leakage induced by AAPH, maintained the integrity of cell structure, enhanced the activities of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx) and therefore oxidative stress resistance, reduced the proportion of sub-G1/G0 cells and necrosis or apoptosis induced by AAPH. These results demonstrated that CA could elevate cellular antioxidant capacity, maintain the integrity of cellular structure, and ultimatelyp rotect HAECs against oxidative stress damage induced byA APH. © 2017, China Food Publishing Company. All right reserved.
Keyword ：
Antioxidants Antioxidants Cell death Cell death Curve fitting Curve fitting Endothelial cells Endothelial cells Enzymes Enzymes Lipoproteins Lipoproteins Oxidation Oxidation Oxidative stress Oxidative stress
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| GB/T 7714 | Li, Feng , Li, Yijia , Li, Qingxian et al. Corosolic Acid from Folium Eriobotryae Inhibits LDL Oxidation and Protects HAECs against Oxidative Damage [J]. | Food Science , 2017 , 38 (15) : 215-220 . |
| MLA | Li, Feng et al. "Corosolic Acid from Folium Eriobotryae Inhibits LDL Oxidation and Protects HAECs against Oxidative Damage" . | Food Science 38 . 15 (2017) : 215-220 . |
| APA | Li, Feng , Li, Yijia , Li, Qingxian , Guo, Yanghao , Shi, Xian'ai . Corosolic Acid from Folium Eriobotryae Inhibits LDL Oxidation and Protects HAECs against Oxidative Damage . | Food Science , 2017 , 38 (15) , 215-220 . |
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